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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Dag1tm1Kcam
targeted mutation 1, Kevin P Campbell
MGI:1934902
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Dag1tm1Kcam/Dag1tm1Kcam involves: 129S1/Sv * 129X1/SvJ * C57BL/6J MGI:3656076
cn2
Dag1tm1Kcam/Dag1tm2.1Kcam
Edil3Tg(Sox2-cre)1Amc/Edil3+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:5470527
cn3
Dag1tm1Kcam/Dag1tm2Kcam
Tg(GFAP-cre)25Mes/0
involves: 129S1/Sv * 129X1/SvJ * FVB/N MGI:2684282


Genotype
MGI:3656076
cn1
Allelic
Composition
Dag1tm1Kcam/Dag1tm1Kcam
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dag1tm1Kcam mutation (1 available); any Dag1 mutation (109 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• most homozygotes are substantially resorbed by E10.5

embryo
• homozygotes fail to gastrulate
• homozygotes fail to progress beyond the early egg cylinder stage of development
• however, early differentiation of extraembryonic lineages and implantation appear unaffected
• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos
• starting at E6.5, homozygotes typically have a significantly smaller embryonic region
• only a small mass of embryonic tissue is detected at E10.5
• at E7.5, homozygotes lack any identifiable mesodermal tissue
• at E6.5, homozygotes lack any significant development of extraembryonic ectoderm
• in contrast, visceral endoderm and a well-formed pro-amniotic cavity are clearly evident
• in contrast, the basement membrane between the visceral endoderm and ectoderm appears normal
• only occasional "patches" of laminin and collagen IV, two major structural elements of Reichert's membrane, are detected
• at E6.5, homozygotes lack a continuous Reichert's membrane; maternal red blood cells are thus found in the yolk sac cavity as early as E5.5
• Reichert's membrane defects are not due to a lack of parietal endoderm

growth/size/body
• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos




Genotype
MGI:5470527
cn2
Allelic
Composition
Dag1tm1Kcam/Dag1tm2.1Kcam
Edil3Tg(Sox2-cre)1Amc/Edil3+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dag1tm1Kcam mutation (1 available); any Dag1 mutation (109 available)
Dag1tm2.1Kcam mutation (1 available); any Dag1 mutation (109 available)
Edil3Tg(Sox2-cre)1Amc mutation (5 available); any Edil3 mutation (43 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice
• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice
• endfoot detachment
• at E11.5, commissural axons exhibit robust postcrossing trajectory defects with failure to project to the lateral portion of the funiculus and altered lateral and ventral funiculi ratio compared with wild-type mice
• at E13, a large number of commissural axons project abnormally within the floor plate unlike in wild-type mice
• at E13.5, mice exhibit extensive disruptions in more lateral aspect of the ventrolateral funiculus compared with wild-type mice
• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

cellular
• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice
• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice
• endfoot detachment
• at E11.5, mice exhibit progressive fragmentation of the basement membrane surrounding the spinal cord which is accompanied by detachment of radial neuroepithelial endfeet from the basal surface unlike wild-type mice




Genotype
MGI:2684282
cn3
Allelic
Composition
Dag1tm1Kcam/Dag1tm2Kcam
Tg(GFAP-cre)25Mes/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dag1tm1Kcam mutation (1 available); any Dag1 mutation (109 available)
Dag1tm2Kcam mutation (2 available); any Dag1 mutation (109 available)
Tg(GFAP-cre)25Mes mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• cerebral, cerebellar and brain stem neuronal migration abnormalities

cellular
• cerebral, cerebellar and brain stem neuronal migration abnormalities

growth/size/body

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
lissencephaly DOID:0050453 OMIM:PS607432
J:86901





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory