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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gt(ROSA)26Sortm2Sho
targeted mutation 2, Stuart Orkin
MGI:2136519
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Lef1tm1Hhx/Lef1tm1Hhx
Tcf7tm1Cle/Tcf7tm1Cle
Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Tg(GZMB-cre)1Jcb/0 		involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * FVB/N MGI:5447982
cn2
 		Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Leprtm1.1Chua/Leprtm1.1Chua
Ntstm1(cre)Mgmj/Nts+ 		involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6 * FVB/N MGI:5297946
cn3
 		Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Sp8tm1Smb/Sp8tm1Smb
H2az2Tg(Wnt1-cre)11Rth/H2az2+ 		involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J * CBA/J MGI:7355998
cn4
 		Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Ntstm1(cre)Mgmj/Nts+ 		involves: 129S4/SvJaeSor * C57BL/6 * FVB MGI:5297948
cn5
 		Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Lef1tm1Hhx/Lef1tm1Hhx
Tg(GZMB-cre)1Jcb/0 		involves: 129S4/SvJaeSor * C57BL/6 * FVB/N MGI:5447981
cn6
 		Gt(ROSA)26Sortm2Sho/?
Tg(Lck-cre)548Jxm/0 		involves: 129S4/SvJaeSor * C57BL/6 * SJL MGI:3696481


Genotype
MGI:5447982
cn1
Allelic
Composition		 Lef1tm1Hhx/Lef1tm1Hhx
Tcf7tm1Cle/Tcf7tm1Cle
Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Tg(GZMB-cre)1Jcb/0
Genetic
Background		 involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
Lef1tm1Hhx mutation (2 available); any Lef1 mutation (43 available)
Tcf7tm1Cle mutation (0 available); any Tcf7 mutation (26 available)
Tg(GZMB-cre)1Jcb mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased CD8-positive, alpha-beta T cell number ( J:189838 )
• decreased CD8+ effector T cells in the peripheral blood lymphocytes compared with control mice and more so than in Tcf7tm1Cle homozygotes following infection with actA- L. monocytogenes-expressing ovalbumin
• decreased memory precursors and memory CD8+ T cells compared with control mice and more so than in Tcf7tm1Cle homozygotes following infection with actA- L. monocytogenes-expressing ovalbumin
abnormal memory T cell physiology ( J:189838 )
• following infection with actA- L. monocytogenes-expressing ovalbumin, memory CD8+ T cells exhibit impaired maturation, function and recall response compared with control mice
decreased tumor necrosis factor secretion ( J:189838 )
• from CD8+ effector T cells following infection with actA- L. monocytogenes-expressing ovalbumin

hematopoietic system
decreased CD8-positive, alpha-beta T cell number ( J:189838 )
• decreased CD8+ effector T cells in the peripheral blood lymphocytes compared with control mice and more so than in Tcf7tm1Cle homozygotes following infection with actA- L. monocytogenes-expressing ovalbumin
• decreased memory precursors and memory CD8+ T cells compared with control mice and more so than in Tcf7tm1Cle homozygotes following infection with actA- L. monocytogenes-expressing ovalbumin
abnormal memory T cell physiology ( J:189838 )
• following infection with actA- L. monocytogenes-expressing ovalbumin, memory CD8+ T cells exhibit impaired maturation, function and recall response compared with control mice




Genotype
MGI:5297946
cn2
Allelic
Composition		 Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Leprtm1.1Chua/Leprtm1.1Chua
Ntstm1(cre)Mgmj/Nts+
Genetic
Background		 involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
Leprtm1.1Chua mutation (1 available); any Lepr mutation (122 available)
Ntstm1(cre)Mgmj mutation (1 available); any Nts mutation (18 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal nervous system electrophysiology ( J:176657 )
• all lateral hypothalamic area (LHA) Nts +ve neurons treated with leptin become hyperpolarized; in controls, about 20% of wild-type LHA Nts +ve neurons treated with leptin while about 60% depolarize upon leptin treatment
• this indicates that deletion of Lepr from Lepr, Nts +ve neurons abrogates the direct depolarization response




Genotype
MGI:7355998
cn3
Allelic
Composition		 Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Sp8tm1Smb/Sp8tm1Smb
H2az2Tg(Wnt1-cre)11Rth/H2az2+
Genetic
Background		 involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J * CBA/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
H2az2Tg(Wnt1-cre)11Rth mutation (2 available); any H2az2 mutation (26 available)
Sp8tm1Smb mutation (0 available); any Sp8 mutation (29 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
embryo
increased cranial neural crest cell apoptosis ( J:200761 )
• at E9.5, a 4-fold increase in apoptosis is detected in the neural crest cells (NCCs) of the facial mesenchyme
• at E10.5, apoptosis is significantly increased in both the medial nasal prominences (MNP) and lateral nasal prominences (LNP) and the olfactory pit
increased head mesenchyme apoptosis ( J:200761 )
• at E9.5, a 4-fold increase in apoptosis is detected in the neural crest cells (NCCs) of the facial mesenchyme
• at E10.5, apoptosis is significantly increased in both the medial nasal prominences (MNP) and lateral nasal prominences (LNP) and the olfactory pit
decreased cranial neural crest cell proliferation ( J:200761 )
• at E9.5, but not at E8.5, NCC proliferation is significantly reduced in the facial mesenchyme
• at E10.5, cell proliferation is significantly reduced in the olfactory pit and the lateral nasal prominences (LNP), but not in the medial nasal prominences (MNP)

nervous system
increased embryonic neuroepithelium apoptosis ( J:200761 )
• at E8.5, a 3-fold increase in apoptosis is detected in the anterior neuroepithelium of the cranial neural folds, including the anterior neural ridge (ANR); however, no elevated apoptosis is observed in the neural crest cells (NCCs) at this age

cellular
increased cranial neural crest cell apoptosis ( J:200761 )
• at E9.5, a 4-fold increase in apoptosis is detected in the neural crest cells (NCCs) of the facial mesenchyme
• at E10.5, apoptosis is significantly increased in both the medial nasal prominences (MNP) and lateral nasal prominences (LNP) and the olfactory pit
increased head mesenchyme apoptosis ( J:200761 )
• at E9.5, a 4-fold increase in apoptosis is detected in the neural crest cells (NCCs) of the facial mesenchyme
• at E10.5, apoptosis is significantly increased in both the medial nasal prominences (MNP) and lateral nasal prominences (LNP) and the olfactory pit
increased embryonic neuroepithelium apoptosis ( J:200761 )
• at E8.5, a 3-fold increase in apoptosis is detected in the anterior neuroepithelium of the cranial neural folds, including the anterior neural ridge (ANR); however, no elevated apoptosis is observed in the neural crest cells (NCCs) at this age
decreased cranial neural crest cell proliferation ( J:200761 )
• at E9.5, but not at E8.5, NCC proliferation is significantly reduced in the facial mesenchyme
• at E10.5, cell proliferation is significantly reduced in the olfactory pit and the lateral nasal prominences (LNP), but not in the medial nasal prominences (MNP)

craniofacial
abnormal nasal pit morphology ( J:200761 )
• at E10.5, apoptosis is significantly increased whereas cell proliferation is significantly reduced in the olfactory pit

growth/size/body
increased head mesenchyme apoptosis ( J:200761 )
• at E9.5, a 4-fold increase in apoptosis is detected in the neural crest cells (NCCs) of the facial mesenchyme
• at E10.5, apoptosis is significantly increased in both the medial nasal prominences (MNP) and lateral nasal prominences (LNP) and the olfactory pit

respiratory system
abnormal nasal pit morphology ( J:200761 )
• at E10.5, apoptosis is significantly increased whereas cell proliferation is significantly reduced in the olfactory pit




Genotype
MGI:5297948
cn4
Allelic
Composition		 Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Ntstm1(cre)Mgmj/Nts+
Genetic
Background		 involves: 129S4/SvJaeSor * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
Ntstm1(cre)Mgmj mutation (1 available); any Nts mutation (18 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
nervous system phenotype ( J:176657 )
N
• 62% of brain slice lateral hypothalamic area (LHA) Nts +ve neurons depolarize when treated with leptin while about 20% of Nts +ve neurons respond with slow hyperpolarization




Genotype
MGI:5447981
cn5
Allelic
Composition		 Gt(ROSA)26Sortm2Sho/Gt(ROSA)26Sor+
Lef1tm1Hhx/Lef1tm1Hhx
Tg(GZMB-cre)1Jcb/0
Genetic
Background		 involves: 129S4/SvJaeSor * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
Lef1tm1Hhx mutation (2 available); any Lef1 mutation (43 available)
Tg(GZMB-cre)1Jcb mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased CD8-positive, alpha-beta T cell number ( J:189838 )
• decreased CD8+ effector T cells in the peripheral blood lymphocytes compared with control mice following infection with actA- L. monocytogenes-expressing ovalbumin
• decreased CD8+ memory precursors and memory T cells compared with control mice following infection with actA- L. monocytogenes-expressing ovalbumin
abnormal memory T cell physiology ( J:189838 )
• following infection with actA- L. monocytogenes-expressing ovalbumin, memory CD8+ T cells exhibit impaired maturation, function and recall response compared with control mice

hematopoietic system
decreased CD8-positive, alpha-beta T cell number ( J:189838 )
• decreased CD8+ effector T cells in the peripheral blood lymphocytes compared with control mice following infection with actA- L. monocytogenes-expressing ovalbumin
• decreased CD8+ memory precursors and memory T cells compared with control mice following infection with actA- L. monocytogenes-expressing ovalbumin
abnormal memory T cell physiology ( J:189838 )
• following infection with actA- L. monocytogenes-expressing ovalbumin, memory CD8+ T cells exhibit impaired maturation, function and recall response compared with control mice




Genotype
MGI:3696481
cn6
Allelic
Composition		 Gt(ROSA)26Sortm2Sho/?
Tg(Lck-cre)548Jxm/0
Genetic
Background		 involves: 129S4/SvJaeSor * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2Sho mutation (2 available); any Gt(ROSA)26Sor mutation (993 available)
Tg(Lck-cre)548Jxm mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:117041 )
• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory