Phenotypes associated with this allele

• Allele Symbol: Hoxa9^tm1Mrc
• Allele Name: targeted mutation 1, Mario R Capecchi
• Allele ID: MGI:2150427
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc	B6.129S7-Hoxa9^tm1Mrc	MGI:3762009
cx2	Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl Hoxa9^tm1Mrc/Hoxa9^tm1Mrc	involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6	MGI:3762008
cx3	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc Hoxb9^tm1Mrc/Hoxb9^tm1Mrc	involves: 129S7/SvEvBrd	MGI:2178666
cx4	Hoxa9^tm1Mrc/Hoxa9^+ Hoxb9^tm1Mrc/Hoxb9^tm1Mrc	involves: 129S7/SvEvBrd	MGI:3612546
cx5	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc Hoxb9^tm1Mrc/Hoxb9^tm1Mrc Hoxc9^tm1Mrc/Hoxc9^tm1Mrc Hoxd9^tm1Mrc/Hoxd9^tm1Mrc	involves: 129S7/SvEvBrd	MGI:4358350
cx6	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc Hoxb9^tm1Mrc/Hoxb9^tm1Mrc Hoxc9^tm1Mrc/Hoxc9^+ Hoxd9^tm1Mrc/Hoxd9^tm1Mrc	involves: 129S7/SvEvBrd	MGI:4358353
cx7	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc Hoxb9^tm1Mrc/Hoxb9^tm1Mrc Hoxd9^tm1Mrc/Hoxd9^tm1Mrc	Not Specified	MGI:3526127
cx8	Hoxa9^tm1Mrc/Hoxa9^+ Hoxb9^tm1Mrc/Hoxb9^tm1Mrc Hoxd9^tm1Mrc/Hoxd9^tm1Mrc	Not Specified	MGI:3526128
cx9	Hoxa9^tm1Mrc/Hoxa9^tm1Mrc Hoxb9^tm1Mrc/Hoxb9^+ Hoxd9^tm1Mrc/Hoxd9^tm1Mrc	Not Specified	MGI:3526129

Genotype
MGI:3762009

hm1

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc
• Genetic Background: B6.129S7-Hoxa9^tm1Mrc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:126371 )

• while the number of colonies formed from cultured bone marrow cells is normal, the size of colonies formed is smaller than in wild-type mice

decreased bone marrow cell number ( J:126371 )

• 4.7+/-0.3x10⁶ cells in 2 femurs compared to 6.0+/-0.4x10⁶ cells in 2 wild-type femurs

increased hematopoietic stem cell number ( J:126371 )

• the number of hematopoietic stem cells (Lin-Sca-1+c-Kit+CD150+ and Lin-Sca-1+c-Kit+CD34-) in bone marrow is increased 2-fold compared to in wild-type mice

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mg in Del(11HoxB3-HoxB4)1Karl Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 15.5+/-0.8x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 8.9+/-0.3x10⁷ cells in Del(11HoxB3-HoxB4)1Karl Hoxa9^tm1Mrc homozygotes

growth/size/body

decreased body weight ( J:126371 )

• 27.6+/-1.0 g compared to 31.5+/-1.2 g for wild-type mice and 22.7+/-0.9 g for Del(11HoxB3-HoxB4)1Karl Hoxa9^tm1Mrc homozygotes

immune system

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mg in Del(11HoxB3-HoxB4)1Karl Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 15.5+/-0.8x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 8.9+/-0.3x10⁷ cells in Del(11HoxB3-HoxB4)1Karl Hoxa9^tm1Mrc homozygotes

Genotype
MGI:3762008

cx2

• Allelic Composition: Del(11Hoxb3-Hoxb4)1Karl/Del(11Hoxb3-Hoxb4)1Karl; Hoxa9^tm1Mrc/Hoxa9^tm1Mrc
• Genetic Background: involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * C57BL/6

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:126371 )

• while the number of colonies formed from cultured bone marrow cells is normal, the size of colonies formed is smaller than in wild-type mice

decreased bone marrow cell number ( J:126371 )

• 4.3+/-0.3x10⁶ cells in 2 femurs compared to 6.0+/-0.4x10⁶ cells in 2 wild-type femurs

• however, red and white blood cell and hematocrit levels are normal

increased hematopoietic stem cell number ( J:126371 )

• the number of hematopoietic stem cells (Lin-Sca-1+c-Kit+CD150+ and Lin-Sca-1+c-Kit+CD34-) in bone marrow is increased 3-fold compared to in wild-type mice

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mgin Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 8.9+/-0.3x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 15.5+/-0.8x10⁷ cells in Hoxa9^tm1Mrc homozygotes

growth/size/body

decreased body weight ( J:126371 )

• 22.7+/-0.9 g compared to 31.5+/-1.2 g for wild-type mice and 27.6+/-1.0 g for Hoxa9^tm1Mrc homozygotes

immune system

decreased spleen weight ( J:126371 )

• 46.3+/-2.3 mg compared to 96.3+/-4.4 g in wild-type mice and 81.4+/-3.2 mgin Hoxa9^tm1Mrc homozygotes

spleen hypoplasia ( J:126371 )

• 8.9+/-0.3x10⁷ cells compared to 22.0+/-2.1x10⁷ cells in wild-type mice and 15.5+/-0.8x10⁷ cells in Hoxa9^tm1Mrc homozygotes

Genotype
MGI:2178666

cx3

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc
• Genetic Background: involves: 129S7/SvEvBrd

skeleton

sternum hypoplasia ( J:37847 )

• sternum is hypoplastic and the length of all the intercostal segments is reduced and malformed

increased rib number ( J:37847 )

• 56% exhibit an extra articulating eighth rib

• 100% exhibit an extra pair of fully-grown ribs on the 21st vertebra

rib fusion ( J:37847 )

• 100 % exhibit bilateral fusions of first and second ribs that are more severe than in single homozygous mutant mice

• third rib fused to first and second rib fusion, incomplete penetrance

Genotype
MGI:3612546

cx4

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9⁺; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc
• Genetic Background: involves: 129S7/SvEvBrd

skeleton

increased rib number ( J:37847 )

• 53% exhibit an extra articulating eighth rib

• 53% exhibit an extra pair of fully-grown ribs on the 21st vertebra

rib fusion ( J:37847 )

• 93% exhibit fusions of first and second ribs

Genotype
MGI:4358350

cx5

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc; Hoxc9^tm1Mrc/Hoxc9^tm1Mrc; Hoxd9^tm1Mrc/Hoxd9^tm1Mrc
• Genetic Background: involves: 129S7/SvEvBrd

limbs/digits/tail

decreased caudal vertebrae number ( J:124112 )

• two fewer than in wild-type mice

skeleton

decreased caudal vertebrae number ( J:124112 )

• two fewer than in wild-type mice

abnormal sternebra morphology ( J:124112 )

• mice lack distinct sternebrae unlike in wild-type mice

abnormal rib joint morphology ( J:124112 )

• the first rib does not always attach to the sternum unlike in wild-type mice

• rib attachment to the sternum continues past T7 to T13 and the transformed 'T14'

abnormal rib development ( J:124112 )

• posterior rib growth is abnormal with 4 vertebrae posterior to T13 forming ribs

rib fusion ( J:124112 )

• distal fusion of anterior-most ribs

small thoracic cage ( J:124112 )

abnormal sacral vertebrae morphology ( J:124112 )

• shifted posteriorly with displacement of pelvic attachment

vertebral transformation ( J:124112 )

• mice exhibit anterior homeotic transformations through the posterior thoracic skeleton and into the lumbar region

• the axial skeleton posterior to the transformations are shifted caudally by two vertebral segments

thoracic vertebral transformation ( J:124112 )

• T8 through L2 exhibit a T7-like phenotype

lumbar vertebral transformation ( J:124112 )

• T8 through L2 exhibit a T7-like phenotype

• L5 has an L3-like phenotype

abnormal sternum ossification ( J:124112 )

• the sternum is ossified and mispatterened along its entire anterior posterior length

Genotype
MGI:4358353

cx6

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc; Hoxc9^tm1Mrc/Hoxc9⁺; Hoxd9^tm1Mrc/Hoxd9^tm1Mrc
• Genetic Background: involves: 129S7/SvEvBrd

skeleton

abnormal rib joint morphology ( J:124112 )

• mice exhibit two extra ribbed vertebrae on T8 and T9 that attach to the sternum

abnormal thoracic vertebrae morphology ( J:124112 )

• mice exhibit two extra ribbed vertebrae on T8 and T9 that attach to the sternum

Genotype
MGI:3526127

cx7

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc; Hoxd9^tm1Mrc/Hoxd9^tm1Mrc
• Genetic Background: Not Specified

endocrine/exocrine glands

abnormal branching of the mammary ductal tree ( J:52279 )

♀ • at day 12.5 of pregnancy branching of the epithelial ductal system is significantly reduced

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration

integument

abnormal branching of the mammary ductal tree ( J:52279 )

♀ • at day 12.5 of pregnancy branching of the epithelial ductal system is significantly reduced

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration

Genotype
MGI:3526128

cx8

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9⁺; Hoxb9^tm1Mrc/Hoxb9^tm1Mrc; Hoxd9^tm1Mrc/Hoxd9^tm1Mrc
• Genetic Background: Not Specified

endocrine/exocrine glands

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration

integument

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration

Genotype
MGI:3526129

cx9

• Allelic Composition: Hoxa9^tm1Mrc/Hoxa9^tm1Mrc; Hoxb9^tm1Mrc/Hoxb9⁺; Hoxd9^tm1Mrc/Hoxd9^tm1Mrc
• Genetic Background: Not Specified

endocrine/exocrine glands

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration

integument

mammary gland hypoplasia ( J:52279 )

♀ • mammary glands in virgin females appear normal, however during and after pregnancy mammary gland hypoplasia as a result of a delay in growth and differentiation of the mammary epithelium is seen

♀ • after parturition reduced numbers of lobuloalveolar structures and large amounts of intracellular fat droplets are seen

abnormal lactation ( J:52279 )

♀ • mutant females have difficulty nursing pups with most pups dying shortly after birth of dehydration