Phenotypes associated with this allele

• Allele Symbol: Hoxa13^tm2Ipc
• Allele Name: targeted mutation 2, I Pierre Chambon
• Allele ID: MGI:2150507
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hoxa13^tm2Ipc/Hoxa13^tm2Ipc	either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721889
ht2	Hoxa13^tm2Ipc/Hoxa13^+	either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721891
cx3	Hoxa13^tm2Ipc/Hoxa13^tm2Ipc Hoxd13^tm1Ddu/Hoxd13^+	either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721898
cx4	Hoxa13^tm2Ipc/Hoxa13^+ Hoxd13^tm1Ddu/Hoxd13^+	either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721893
cx5	Hoxa13^tm2Ipc/Hoxa13^+ Hoxd13^tm1Ddu/Hoxd13^tm1Ddu	either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721896
cx6	Hoxa13^tm2Ipc/Hoxa13^tm2Ipc Hoxd13^tm1Ddu/Hoxd13^tm1Ddu	either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)	MGI:3721901

Genotype
MGI:3721889

hm1

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13^tm2Ipc
• Genetic Background: either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:36214 )

• pups die between E11.5 and E15.5

limbs/digits/tail

abnormal carpal bone morphology ( J:36214 )

• at E13.5, no distinct carpal condensation, with the exception of that for distal carpal 4, is detected

• at E15.5, the pyramidal is rudimentary, the central and distal carpals are poorly developed and there is no discernible scapholunatum cartilage

abnormal digit morphology ( J:36214 )

• at E13.5, the fibula remains connected to digit V in the hindlimbs mice lack digit I

• at E14.5, remaining digits are not properly separated

• at E15.5, digits II and V in the forelimb and all digits in the hindlimb lack the second phalanges cartilages

abnormal phalanx morphology ( J:36214 )

• at E15.5, digits II and V in the forelimb and all digits in the hindlimb lack the second phalanges cartilages

brachydactyly ( J:36214 )

• digits II, III and V of the forelimbs and hindlimbs are reduced in size

clinodactyly ( J:36214 )

• at E15.5, digits bend towards the anterior side

oligodactyly ( J:36214 )

• at E14.5 and E15.5, digit 1 is absent from the forelimbs and hindlimbs

interdigital webbing ( J:36214 )

• at E15.5, webbing between digits II and III is present digit 1 is absent from the forelimbs and hindlimbs

abnormal tarsal bone morphology ( J:36214 )

• at E13.5, no tarsus elements are individualized and the fibula remains connected to digit V

• at E15.5, all tarsal elements are poorly modeled

abnormal fibula morphology ( J:36214 )

• at E13.5, the fibula remains connected to digit V in the hindlimbs

abnormal limb development ( J:36214 )

abnormal digit development ( J:36214 )

• at E13.5, mice have only developed 4 digit anlagen

• at E13.5, no protrusion corresponding to digit 1 is evident and condensation for digit I is absent in the forelimbs and hindlimbs

• until E15.5, digit V in the hindlimb is a small and thin primary condensation but eventually develops normally

abnormal embryonic autopod plate morphology ( J:36214 )

• at E12.5, the foot plates are narrower than in wild-type mice and abnormally flat along the anterior margin

• at E13.5, the foot plates are narrower than in wild-type mice and have only developed 4 digit anlagen

skeleton

abnormal carpal bone morphology ( J:36214 )

• at E13.5, no distinct carpal condensation, with the exception of that for distal carpal 4, is detected

• at E15.5, the pyramidal is rudimentary, the central and distal carpals are poorly developed and there is no discernible scapholunatum cartilage

abnormal phalanx morphology ( J:36214 )

• at E15.5, digits II and V in the forelimb and all digits in the hindlimb lack the second phalanges cartilages

abnormal tarsal bone morphology ( J:36214 )

• at E13.5, no tarsus elements are individualized and the fibula remains connected to digit V

• at E15.5, all tarsal elements are poorly modeled

abnormal fibula morphology ( J:36214 )

• at E13.5, the fibula remains connected to digit V in the hindlimbs

Genotype
MGI:3721891

ht2

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13⁺
• Genetic Background: either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

limbs/digits/tail

abnormal digit morphology ( J:36214 )

• digit I shows an incomplete segmentation between the two phalangeal cartilages but no truncation

• in digit I of the hindlimb, the terminal phalanx has an altered bell-shaped morphology

abnormal phalanx morphology ( J:36214 )

• digit I shows an incomplete segmentation between the two phalangeal cartilages but no truncation

• in digit I of the hindlimb, the terminal phalanx has an altered bell-shaped morphology

interdigital webbing ( J:36214 )

• some mice have an interdigital fusion in digits II and III

skeleton

abnormal phalanx morphology ( J:36214 )

• digit I shows an incomplete segmentation between the two phalangeal cartilages but no truncation

• in digit I of the hindlimb, the terminal phalanx has an altered bell-shaped morphology

Genotype
MGI:3721898

cx3

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13^tm2Ipc; Hoxd13^tm1Ddu/Hoxd13⁺
• Genetic Background: either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:36214 )

• mice are not recovered after E14.5

limbs/digits/tail

abnormal carpal bone morphology ( J:36214 )

• with the exception of an altered pisiform cartilage, there is no individualization of carpal condensation

abnormal digit morphology ( J:36214 )

• digits lack separation and segmentation and tend to fuse terminally

• mice lack digit I and some mice have a minute posterior extra digit cartilage

abnormal tarsal bone morphology ( J:36214 )

• the tarsus is altered more than in Hoxa13^tm2Ipc/ Hoxa13⁺ Hoxd13^tm1Ddu/Hoxd13^tm1Ddu mice

• none of the tarsus blastemas are properly individualized and there is no separation between proximal and distal rows

abnormal digit development ( J:36214 )

• in the hindlimbs condensations for digits I through V are apparent but are irregular and remain in a primitive state

• in the hindlimbs, there is no chondrification in digit rays II and V

abnormal embryonic autopod plate morphology ( J:36214 )

• foot plates are narrower, especially towards the distal extremities, when compared with Hoxa13^tm2Ipc/ Hoxa13⁺ Hoxd13^tm1Ddu/Hoxd13^tm1Ddu mice

skeleton

abnormal carpal bone morphology ( J:36214 )

• with the exception of an altered pisiform cartilage, there is no individualization of carpal condensation

abnormal tarsal bone morphology ( J:36214 )

• the tarsus is altered more than in Hoxa13^tm2Ipc/ Hoxa13⁺ Hoxd13^tm1Ddu/Hoxd13^tm1Ddu mice

• none of the tarsus blastemas are properly individualized and there is no separation between proximal and distal rows

Genotype
MGI:3721893

cx4

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13⁺; Hoxd13^tm1Ddu/Hoxd13⁺
• Genetic Background: either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

limbs/digits/tail

abnormal carpal bone morphology ( J:36214 )

• in 50% of mice, the fourth posterior distal carpal bone is partly or fully split into two distinct bone

abnormal digit morphology ( J:36214 )

• digits I and V are deformed in the hinblimb

• digit I is more truncated than normal and no first phalanx is visible

• digit I in the hindlimb has its first phalanx truncated and the terminal phalanx has an altered bell-shaped morphology

• the second phalanges of digits II and mostly in digit V are truncated in the forelimbs and hindlimbs

• digits II and III are fused and alterations in the claw of digit I are observeddigits II and III are fused and alterations in the claw of digit I are observed

• newborn mice show an incomplete segmentation between phalangeal cartilage in digit I

abnormal phalanx morphology ( J:36214 )

• digit I lacks a first phalanx and newborn mice show an incomplete segmentation between the two phalangeal cartilages in forelimbs and in some hindlimbs

• digit I in the hindlimb has its first phalanx truncated and the terminal phalanx has an altered bell-shaped morphology

• the second phalanges of digits II and mostly in digit V are truncated in the forelimbs and hindlimbs

brachydactyly ( J:36214 )

• in adults, digits I, II and V are truncated

polydactyly ( J:36214 )

• 23 of 40 mice have an extra posterior digit in the forelimbs consisting of either a small floating bone distal to the post minimus or an entire small digit

interdigital webbing ( J:36214 )

• in adults, digits III and IV are partially fused in the forelimbs and digits II, III and IV are completely fused in the hindlimbs

elongated metatarsal bones ( J:36214 )

• the metatarsal of digit I is elongated

skeleton

abnormal carpal bone morphology ( J:36214 )

• in 50% of mice, the fourth posterior distal carpal bone is partly or fully split into two distinct bone

abnormal phalanx morphology ( J:36214 )

• digit I lacks a first phalanx and newborn mice show an incomplete segmentation between the two phalangeal cartilages in forelimbs and in some hindlimbs

• digit I in the hindlimb has its first phalanx truncated and the terminal phalanx has an altered bell-shaped morphology

• the second phalanges of digits II and mostly in digit V are truncated in the forelimbs and hindlimbs

elongated metatarsal bones ( J:36214 )

• the metatarsal of digit I is elongated

Genotype
MGI:3721896

cx5

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13⁺; Hoxd13^tm1Ddu/Hoxd13^tm1Ddu
• Genetic Background: either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

limbs/digits/tail

abnormal autopod morphology ( J:36214 )

• digits of the forelimb autopods consists of short metacarpal/phalangeal bones fused together and terminal phalangeal-like structures are fused to form a common terminal arch

decreased autopod size ( J:36214 )

• the forelimb autopod is drastically truncated

abnormal carpal bone morphology ( J:36214 )

• at one week of age, the cartilage has abnormally large central blastema and extra distal cartilage related to the presence of extra digits

fused carpal bones ( J:36214 )

• the distal carpals are fused

abnormal digit morphology ( J:36214 )

• terminal phalanges are partly fused in digits II through V in the hindlimbs

• at E18.5, forelimbs show selective truncation and delay in the ossification of digits II and V and a rudimentary posterior extra digit cartilage

abnormal phalanx morphology ( J:36214 )

• no individual metatarsal and phalangeal bones are observed in the hindlimbs

• terminal phalanges are partly fused in digits II through V in the hindlimbs of some mice

• at E18.5, forelimbs lack segmentation between certain metacarpals and first phalangeal cartilage

brachydactyly ( J:36214 )

• digits of the forelimb autopods consists of short metacarpal/phalangeal bones fused together and terminal phalangeal-like structures are fused to form a common terminal arch

monodactyly ( J:36214 )

• no individual digits are observed and the extremities of the forepaws are a continuous ridge

fused tarsal bones ( J:36214 )

• the distal tarsals are fused

abnormal metacarpal bone morphology ( J:36214 )

• no individual metatarsal and phalangeal bones are observed in the hindlimbs

• at E18.5, forelimbs lack segmentation between certain metacarpals and first phalangeal cartilage

abnormal digit development ( J:36214 )

• at E13.5, an extra digit condensation is observed

• at E13.5, a central condensation is fused distally to one of its neighbors

• an abnormal condensation occurs between digit IV and V condensations

• at E14.5, a seventh posterior digit condensation is observed and a third digit condensation is prematurely truncated or joined distally to the second cartilage

• at E18.5, none of the digits become properly separated half of the mice have central digits fused with one or both of its neighbors

• the size of the digit cartilages decreases towards the anterior and posterior margins and the entire forefoot has a symmetrical round shape

• at E18.5, hindlimb digit cartilages are severely truncated and almost not segmented with terminal fusions

• at E18.5, forelimbs have seven non-ossified digits

skeleton

abnormal carpal bone morphology ( J:36214 )

• at one week of age, the cartilage has abnormally large central blastema and extra distal cartilage related to the presence of extra digits

fused carpal bones ( J:36214 )

• the distal carpals are fused

abnormal phalanx morphology ( J:36214 )

• no individual metatarsal and phalangeal bones are observed in the hindlimbs

• terminal phalanges are partly fused in digits II through V in the hindlimbs of some mice

• at E18.5, forelimbs lack segmentation between certain metacarpals and first phalangeal cartilage

fused tarsal bones ( J:36214 )

• the distal tarsals are fused

abnormal metacarpal bone morphology ( J:36214 )

• no individual metatarsal and phalangeal bones are observed in the hindlimbs

• at E18.5, forelimbs lack segmentation between certain metacarpals and first phalangeal cartilage

Genotype
MGI:3721901

cx6

• Allelic Composition: Hoxa13^tm2Ipc/Hoxa13^tm2Ipc; Hoxd13^tm1Ddu/Hoxd13^tm1Ddu
• Genetic Background: either: (involves: 129/Sv * 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6)

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:36214 )

• pups die between E11.5 and E15.5 similar to in Hoxa13^tm2Ipc homozygotes

limbs/digits/tail

abnormal autopod morphology ( J:36214 )

• at E12.5 to E14.5, autopods are round with no external sign of digit formation and are more truncated than in any Hoxa13^tm2Ipc and/or Hoxd13^tm1Ddu mice

• at E14.5, chondrogenic activity is diffuse throughout the hindlimb autopods and in two ill-defined areas in the forelimb autopods

• in one mouse that survived to E16.5, in the hindlimb there is a cartilage connection between the autopod and fibula

short radius ( J:36214 )

• in three mouse that survived to E16.5

short ulna ( J:36214 )

• in three mouse that survived to E16.5

abnormal fibula morphology ( J:36214 )

• in three mouse that survived to E16.5, in the hindlimb there is a cartilage connection between the autopod and fibula

short limbs ( J:36214 )

growth/size/body

decreased embryo size ( J:36214 )

skeleton

short radius ( J:36214 )

• in three mouse that survived to E16.5

short ulna ( J:36214 )

• in three mouse that survived to E16.5

abnormal fibula morphology ( J:36214 )

• in three mouse that survived to E16.5, in the hindlimb there is a cartilage connection between the autopod and fibula

embryo

decreased embryo size ( J:36214 )