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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Dock2tm1Tsas
targeted mutation 1, Takehiko Sasazuki
MGI:2152031
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Dock2tm1Tsas/Dock2tm1Tsas B6.Cg-Dock2tm1Tsas MGI:3772946
hm2
 		Dock2tm1Tsas/Dock2tm1Tsas involves: C57BL/6 MGI:3690239
cx3
 		Dock2tm1Tsas/Dock2tm1Tsas
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/? 		involves: C57BL/6 MGI:3690240


Genotype
MGI:3772946
hm1
Allelic
Composition		 Dock2tm1Tsas/Dock2tm1Tsas
Genetic
Background		 B6.Cg-Dock2tm1Tsas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dock2tm1Tsas mutation (1 available); any Dock2 mutation (143 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased susceptibility to xenobiotic induced morbidity/mortality ( J:130919 )
• survival following exposure to 150 mg/kg 5-fluorouracil (5-FU) is lower than for wild-type mice
• however, survival following exposure to 250 mg/kg 5-FU mice survive as long as wild-type mice (7 months)

hematopoietic system
impaired neutrophil chemotaxis ( J:113266 )
• when stimulated with fMLP or compliment factor 5a in a transwell chemotaxis assay, less than 2% of neutrophils migrate to the lower chamber compared to 8% to 11% of wild-type cells
• when stimulated with a gradient of fMLP in a Zigmond chamber, neutrophils exhibit a 55% reduction in average speed compared to wild-type cells and cells display indecisive wandering behavior rather than traveling in a straight line
• during migration, direction change and straightness of neutrophil travel are abnormal
abnormal hematopoietic system morphology/development ( J:130919 )
• while bone marrow transplants are capable of hematopoeitic stem cell engraftment and self-renewal, repopulation at the pro-T and B cell level is defective
decreased lymphocyte cell number ( J:130919 )
• the number of mature CD3+ T cells and B220+IgM+ B cells is slightly decreased compared to in wild-type mice
• however, the number of immature B cells, hematopoietic stem cells and common lymphoid progenitors is normal
decreased T cell number ( J:130919 )
• the number of mature CD3+ T cells is slightly decreased compared to in wild-type mice

cellular
abnormal cell migration ( J:130919 )
• when stimulated with CXCL2, lineage-negative colony-forming units and LSK cells exhibit reduced migration compared to wild-type cells
impaired neutrophil chemotaxis ( J:113266 )
• when stimulated with fMLP or compliment factor 5a in a transwell chemotaxis assay, less than 2% of neutrophils migrate to the lower chamber compared to 8% to 11% of wild-type cells
• when stimulated with a gradient of fMLP in a Zigmond chamber, neutrophils exhibit a 55% reduction in average speed compared to wild-type cells and cells display indecisive wandering behavior rather than traveling in a straight line
• during migration, direction change and straightness of neutrophil travel are abnormal

immune system
impaired neutrophil chemotaxis ( J:113266 )
• when stimulated with fMLP or compliment factor 5a in a transwell chemotaxis assay, less than 2% of neutrophils migrate to the lower chamber compared to 8% to 11% of wild-type cells
• when stimulated with a gradient of fMLP in a Zigmond chamber, neutrophils exhibit a 55% reduction in average speed compared to wild-type cells and cells display indecisive wandering behavior rather than traveling in a straight line
• during migration, direction change and straightness of neutrophil travel are abnormal
decreased lymphocyte cell number ( J:130919 )
• the number of mature CD3+ T cells and B220+IgM+ B cells is slightly decreased compared to in wild-type mice
• however, the number of immature B cells, hematopoietic stem cells and common lymphoid progenitors is normal
decreased T cell number ( J:130919 )
• the number of mature CD3+ T cells is slightly decreased compared to in wild-type mice

homeostasis/metabolism
increased susceptibility to xenobiotic induced morbidity/mortality ( J:130919 )
• survival following exposure to 150 mg/kg 5-fluorouracil (5-FU) is lower than for wild-type mice
• however, survival following exposure to 250 mg/kg 5-FU mice survive as long as wild-type mice (7 months)




Genotype
MGI:3690239
hm2
Allelic
Composition		 Dock2tm1Tsas/Dock2tm1Tsas
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dock2tm1Tsas mutation (1 available); any Dock2 mutation (143 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal leukocyte migration ( J:71005 )
• migration to lymph nodes reduced
• migration to spleen reduced somewhat as well
spleen hypoplasia ( J:71005 )
• total number of spleen cells about 50% normal
• proportions of CD4+, CD8+, and B cells are normal
• number of monocytes is significantly increased
abnormal spleen white pulp morphology ( J:71005 )
• lymphoid follicles are atrophied and T and B cells are scattered in the red pulp
decreased marginal zone B cell number ( J:71005 )
• marginal zone B cells are markedly reduced
lymph node hypoplasia ( J:71005 )
• overall reduced numbers of T and B cells
• CD4+ T cell number is significantly reduced

hematopoietic system
abnormal leukocyte migration ( J:71005 )
• migration to lymph nodes reduced
• migration to spleen reduced somewhat as well
spleen hypoplasia ( J:71005 )
• total number of spleen cells about 50% normal
• proportions of CD4+, CD8+, and B cells are normal
• number of monocytes is significantly increased
abnormal spleen white pulp morphology ( J:71005 )
• lymphoid follicles are atrophied and T and B cells are scattered in the red pulp
decreased marginal zone B cell number ( J:71005 )
• marginal zone B cells are markedly reduced

cellular
abnormal leukocyte migration ( J:71005 )
• migration to lymph nodes reduced
• migration to spleen reduced somewhat as well




Genotype
MGI:3690240
cx3
Allelic
Composition		 Dock2tm1Tsas/Dock2tm1Tsas
Tg(Tcra2B4)1Mmd/?
Tg(Tcrb2B4)1Mmd/?
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dock2tm1Tsas mutation (1 available); any Dock2 mutation (143 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased double-positive T cell number ( J:84581 )
• numbers reduced to about 3% of what is seen in controls
• more susceptible to apoptosis
abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )
• reduced in number and ratio in relation to CD8+ T cells is also reduced
abnormal T cell physiology ( J:84581 )
• proliferative responses are reduced

hematopoietic system
decreased double-positive T cell number ( J:84581 )
• numbers reduced to about 3% of what is seen in controls
• more susceptible to apoptosis
abnormal CD4-positive, alpha beta T cell morphology ( J:84581 )
• reduced in number and ratio in relation to CD8+ T cells is also reduced
abnormal T cell physiology ( J:84581 )
• proliferative responses are reduced




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory