Phenotypes associated with this allele

• Allele Symbol: Cul1^tm1Rsjo
• Allele Name: targeted mutation 1, Randall S Johnson
• Allele ID: MGI:2152042
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cul1^tm1Rsjo/Cul1^tm1Rsjo	involves: 129S1/Sv * 129X1/SvJ	MGI:3044891

Genotype
MGI:3044891

hm1

• Allelic Composition: Cul1^tm1Rsjo/Cul1^tm1Rsjo
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:57767 )

• mutant embryos implant in the uterine wall, but fail to develop past E5.5

• as a result, mutant embryos are almost completely resorbed by E7.5

embryo

abnormal ectoderm development ( J:57767 )

• despite their developmental arrest past E5.5, cells of the embryonic ectoderm actively enter S phase and undergo DNA replication as late as E6.5

• at E6.5, p53 and apoptosis are increased in the mutant embryonic ectoderm

decreased embryo size ( J:57767 )

• at E7.5, mutant embryos are much smaller than wild-type, and show no change in morphology from E5.5

growth/size/body

decreased embryo size ( J:57767 )

• at E7.5, mutant embryos are much smaller than wild-type, and show no change in morphology from E5.5

cellular

abnormal cell physiology ( J:57767 )

• at E5.5, mutant embryos exhibit a global increase in cyclin E1 expression

• abnormal accumulation of cyclin E1 is noted in every embryonic tissue layer and is primarily nuclear

• at E5.5, in situ analysis shows no detectable increase in Ccne1 mRNA levels, suggesting a post-transcriptional defect