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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Vcam1tm1Dmil
targeted mutation 1, David Milstone
MGI:2153831
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Vcam1tm1Dmil/Vcam1tm1Dmil C.129S4-Vcam1tm1Dmil MGI:3620569
hm2
 		Vcam1tm1Dmil/Vcam1tm1Dmil either: B6.129S4-Vcam1tm1Dmil or (involves: 129S4/SvJae) MGI:3620571
hm3
 		Vcam1tm1Dmil/Vcam1tm1Dmil either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6) MGI:3620568
hm4
 		Vcam1tm1Dmil/Vcam1tm1Dmil involves: 129S4/SvJae MGI:5701416
cx5
 		Icam1tm1Jcgr/Icam1tm1Jcgr
Ldlrtm1Her/Ldlrtm1Her
Vcam1tm1Dmil/Vcam1tm1Dmil 		involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6 MGI:3620574
cx6
 		Ldlrtm1Her/Ldlrtm1Her
Vcam1tm1Dmil/Vcam1tm1Dmil 		involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6 MGI:3620572
cx7
 		Icam1tm1Jcgr/Icam1tm1Jcgr
Vcam1tm1Dmil/Vcam1tm1Dmil 		involves: 129S4/SvJae * C57BL/6 MGI:5701417
cx8
 		Icam1tm1Jcgr/Icam1tm1Jcgr
Vcam1tm1Dmil/Vcam1+ 		involves: 129S4/SvJae * C57BL/6 MGI:5701418


Genotype
MGI:3620569
hm1
Allelic
Composition		 Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 C.129S4-Vcam1tm1Dmil
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, incomplete penetrance ( J:69597 )
• Background Sensitivity: 24-25% embryonic survival; embryonic lethality increases on a 129 or C57BL/6 congenic background

cellular
decreased cell chemotaxis ( J:227109 )
• reduced recruitment of leukocytes into peritoneal cavity as compared to wild-type following IP challenge with ovalbumin
• numbers of monocytes, macrophages and lymphocytes recovered from the peritoneal cavity are reduced, but not neutrophils or eosinophils
• total cell numbers are not reduced

embryo
abnormal chorioallantoic fusion ( J:69597 )
• more than 50% of homozygotes at E9.5 and E12.5 display chorioallantoic fusion defects

immune system
increased leukocyte cell number ( J:69597 )
• blood leukocytes are elevated 1.6 and 1.4 fold at 8 months and 15 months of age, respectively
increased B cell number ( J:69597 )
• absolute numbers of B lymphocytes are elevated at 8 months of age
increased T cell number ( J:69597 )
• absolute numbers of T lymphocytes are elevated at 8 months of age
increased monocyte cell number ( J:69597 )
• absolute numbers of monocytes are elevated at 8 months of age

hematopoietic system
increased leukocyte cell number ( J:69597 )
• blood leukocytes are elevated 1.6 and 1.4 fold at 8 months and 15 months of age, respectively
increased B cell number ( J:69597 )
• absolute numbers of B lymphocytes are elevated at 8 months of age
increased T cell number ( J:69597 )
• absolute numbers of T lymphocytes are elevated at 8 months of age
increased monocyte cell number ( J:69597 )
• absolute numbers of monocytes are elevated at 8 months of age




Genotype
MGI:3620571
hm2
Allelic
Composition		 Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 either: B6.129S4-Vcam1tm1Dmil or (involves: 129S4/SvJae)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, incomplete penetrance ( J:69597 )
• Background Sensitivity: 6% embryonic survival, which is lower than in mixed 129S4-C57BL/6 or 129S4-BALB/c backgrounds or on a BALB/c congenic background

embryo
abnormal chorioallantoic fusion ( J:69597 )
• more than 50% of homozygotes at E9.5 and E12.5 display chorioallantoic fusion defects




Genotype
MGI:3620568
hm3
Allelic
Composition		 Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, incomplete penetrance ( J:69597 )
• Background Sensitivity: 29% embryonic survival; embryonic lethality increases on a 129 or C57BL/6 congenic background

embryo
abnormal chorioallantoic fusion ( J:69597 )
• more than 50% of homozygotes at E9.5 and E12.5 display chorioallantoic fusion defects

hematopoietic system
increased monocyte cell number ( J:69597 )
• small yet significant elevation in monocytes at 2-4 months of age

immune system
increased monocyte cell number ( J:69597 )
• small yet significant elevation in monocytes at 2-4 months of age




Genotype
MGI:5701416
hm4
Allelic
Composition		 Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased cell chemotaxis ( J:227109 )
• reduced recruitment of lymphocytes into peritoneal cavity as compared to wild-type following IP challenge with ovalbumi
• however, total cell numbers are not reduced




Genotype
MGI:3620574
cx5
Allelic
Composition		 Icam1tm1Jcgr/Icam1tm1Jcgr
Ldlrtm1Her/Ldlrtm1Her
Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Icam1tm1Jcgr mutation (3 available); any Icam1 mutation (25 available)
Ldlrtm1Her mutation (21 available); any Ldlr mutation (83 available)
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
decreased susceptibility to atherosclerosis ( J:69597 )
• after an 8-week cholesterol-enriched diet, whole aorta and arch lesion area is reduced by 31% and 45%, respectively, compared to double homozygous Icam1 and Ldlr mice, and by 48% and 38%, respectively, compared to single homozygous Ldlr mutant mice

homeostasis/metabolism
decreased circulating cholesterol level ( J:69597 )
• after an 8-week cholesterol-enriched diet, develop a less severe hypercholesterolemia than single homozygous Ldlr mutant mice

immune system
increased leukocyte cell number ( J:69597 )
• total leukocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased neutrophil cell number ( J:69597 )
• neutrophil counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased lymphocyte cell number ( J:69597 )
• lymphocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased monocyte cell number ( J:69597 )
• monocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice

hematopoietic system
increased leukocyte cell number ( J:69597 )
• total leukocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased neutrophil cell number ( J:69597 )
• neutrophil counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased lymphocyte cell number ( J:69597 )
• lymphocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice
increased monocyte cell number ( J:69597 )
• monocyte counts are increased when mice have been on an 8-week cholesterol-enriched diet compared to homozygous Ldlr mutant mice




Genotype
MGI:3620572
cx6
Allelic
Composition		 Ldlrtm1Her/Ldlrtm1Her
Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ldlrtm1Her mutation (21 available); any Ldlr mutation (83 available)
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
decreased susceptibility to atherosclerosis ( J:69597 )
• develop fewer early foam cell lesions in the aorta than homozygous Ldlr mice after an 8 week cholesterol-enriched diet, however cholesterol levels, lipoprotein profiles and leukocyte numbers are similar to wild-type




Genotype
MGI:5701417
cx7
Allelic
Composition		 Icam1tm1Jcgr/Icam1tm1Jcgr
Vcam1tm1Dmil/Vcam1tm1Dmil
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Icam1tm1Jcgr mutation (3 available); any Icam1 mutation (25 available)
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
impaired macrophage chemotaxis ( J:227109 )
• reduced recruitment of monocytes/macrophages into peritoneal cavity as compared to wild-type following IP challenge with thioglycollate
decreased cell chemotaxis ( J:227109 )
• reduced recruitment of leukocytes into peritoneal cavity as compared to wild-type and heterozygous Vcam1tmDmil following IP challenge with ovalbumin
• numbers of monocytes, macrophages and lymphocytes recovered from the peritoneal cavity are reduced, but not neutrophils or eosinophils

hematopoietic system
impaired macrophage chemotaxis ( J:227109 )
• reduced recruitment of monocytes/macrophages into peritoneal cavity as compared to wild-type following IP challenge with thioglycollate

immune system
impaired macrophage chemotaxis ( J:227109 )
• reduced recruitment of monocytes/macrophages into peritoneal cavity as compared to wild-type following IP challenge with thioglycollate




Genotype
MGI:5701418
cx8
Allelic
Composition		 Icam1tm1Jcgr/Icam1tm1Jcgr
Vcam1tm1Dmil/Vcam1+
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Icam1tm1Jcgr mutation (3 available); any Icam1 mutation (25 available)
Vcam1tm1Dmil mutation (0 available); any Vcam1 mutation (44 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased cell chemotaxis ( J:227109 )
• reduced recruitment of leukocytes into peritoneal cavity as compared to wild-type, but not homozygote, following IP challenge with ovalbumin
• numbers of monocytes, macrophages and lymphocytes recovered from the peritoneal cavity are reduced, but not neutrophils or eosinophils




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory