Analysis Tools
mortality/aging
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• homozygous null embryos died by midgestation; no viable embryos were found after E10.5
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cellular
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• mutant cells showed reduced caspase-3 activation; caspase-3 activating activity could be rescued by exogenous cytochrome c
• mutants cells were resistant to the proapoptotic effects of UV irradiation, serum withdrawal, or staurosporine
• mutants cells showed increased sensitivity to cell death signals triggered by TNF, both in the presence or absence of cycloheximide
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• cells derived from E8.5 mutant embryos showed respiratory insufficiency and enhanced anaerobic glycolysis
• cell viability and proliferation improved by supplementing the medium with uridine and pyruvate
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embryo
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• up to E9.5, development appeared to progress in a relatively normal but delayed manner
• at E9.5, mutant embryos displayed a primitive heart tube, somites and an allantois, features consistent with ~E8 in wild-type
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• by E8.5, homozygous null embryos had a strikingly reduced size
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• at E9.5, mutant embryos were enclosed in a ball-like structure formed by the yolk sac and the amnion
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growth/size/body
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• up to E9.5, development appeared to progress in a relatively normal but delayed manner
• at E9.5, mutant embryos displayed a primitive heart tube, somites and an allantois, features consistent with ~E8 in wild-type
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• by E8.5, homozygous null embryos had a strikingly reduced size
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