Phenotypes associated with this allele

• Allele Symbol: Uba3^tm1Tchi
• Allele Name: targeted mutation 1, Tomoki Chiba
• Allele ID: MGI:2154503
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Uba3^tm1Tchi/Uba3^tm1Tchi	involves: C57BL/6 * CBA	MGI:2654497

Genotype
MGI:2654497

hm1

• Allelic Composition: Uba3^tm1Tchi/Uba3^tm1Tchi
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, incomplete penetrance ( J:72808 )

• ~50% of homozygous mutant embryos die before implantation

embryonic lethality between implantation and somite formation, complete penetrance ( J:72808 )

• no homozygous mutant embryos are detected at E7.5; instead, only completely absorbed embryonic tissues are identified in the decidua

embryo

abnormal inner cell mass apoptosis ( J:72808 )

• at E5.5, mutant but not wild-type embryos undergo selective apoptosis of the inner cell mass

• notably, mutant trophoblastic cells escape apoptosis and remain viable as late as E6.5

abnormal blastocyst formation ( J:72808 )

• ~50% of homozygous mutant embryos show defective blastocyst formation

abnormal blastocyst hatching ( J:72808 )

• ~50% of homozygous mutant embryos display defects in hatching

abnormal embryonic tissue morphology ( J:72808 )

• at E6.0, most mutant embryos consist of extraembryonic tissue

absent embryonic epiblast ( J:72808 )

• at E6.0, most mutant embryos lack an embryonic ectoderm

disorganized embryonic tissue ( J:72808 )

• at E6.0-E6.5, remaining mutant embryos appear disorganized and lack the cylinder-like two-layered cellular structure observed in wild-type embryos

abnormal inner cell mass morphology ( J:72808 )

• an atrophic inner cell mass is observed

cellular

abnormal cell cycle ( J:72808 )

• mutant trophoblastic cells cannot enter the S phase of the endoreduplication cycle

• this cell cycle arrest is accompanied with elevated expression of cyclin E and p57Kip2, suggesting that both mitotic and the endoreduplicative cell cycle progression are affected

abnormal inner cell mass apoptosis ( J:72808 )

• at E5.5, mutant but not wild-type embryos undergo selective apoptosis of the inner cell mass

• notably, mutant trophoblastic cells escape apoptosis and remain viable as late as E6.5