Phenotypes associated with this allele

• Allele Symbol: Plcd4^tm1Kfu
• Allele Name: targeted mutation 1, Kiyoko Fukami
• Allele ID: MGI:2155167
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcd4^tm1Kfu/Plcd4^tm1Kfu	involves: 129X1/SvJ * C57BL/6J	MGI:3038375

Genotype
MGI:3038375

hm1

• Allelic Composition: Plcd4^tm1Kfu/Plcd4^tm1Kfu
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:69105 )

N • spermatogenesis proceeded normally, as indicated by the presence and production of mature sperm

N • microscopy revealed that epididymal sperm from mutant males were similar in number, viability, morphology, and motility to sperm from wild-type males

male infertility ( J:69105 , J:82910 )

♂ • homozygous mutant male mice either produced few small litters or were sterile; in contrast, homozygous mutant females appeared to be fertile (J:69105)

♂ • in vitro fertilization experiments revealed that insemination with mutant sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed (J:69105)

♂ (J:82910)

abnormal sperm physiology ( J:82910 )

♂ • mutant sperm exhibited normal capacitation events, but failed to exhibit the expected intracellular [Ca2+]i mobilization in response to ZP

♂ • progesterone, another physiological inducer of the acrosome reaction, failed to induce sustained [Ca2+]i increases in mutant sperm, also resulting in partial inhibition of the acrosome reaction

♂ • finally, assessment of store-operated channel (SOC) function using thapsigargin indicated that capacitative Ca2+ entry through SOC channels was severely impaired in mutant sperm

impaired acrosome reaction ( J:69105 , J:82910 )

♂ • mutant sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the zona pellucida (ZP) (J:69105)

♂ • progesterone, another physiological inducer of the acrosome reaction, failed to induce sustained [Ca2+]i increases in mutant sperm, also resulting in partial inhibition of the acrosome reaction (J:82910)