Analysis Tools
hematopoietic system
immune system
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• normal in vitro survival assays with several cytotoxic stimuli on sorted thymocyte and B cell populations
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• increased spleen weight (>2.5 fold)
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• irradiated mice reconstituted with mutant fetal liver cells have a 2- to 4- fold increase in lymphocyte and myeloid cell numbers compared to controls
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• T1 transitional B cells are insignificantly elevated
• increased T1 transitional B cells in inguinal lymph nodes
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• increased white blood cells numbers (>3 fold)
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• at 6 months
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• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells
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• mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to wild-type cells
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• about 10-fold more T cells cultured without cytokines are alive after six days compared to controls
(J:73316)
• double positive T cells with increased resistance to the apoptotic effects of dexamethasone
(J:133084)
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• following infection with mouse cytomegalovirus infection, more NK cell survive at day 10 and 14 than in wild-type mice
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• NK cells are more resistant to LY294002-induced apoptosis
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• NK cells are more resistant to LY294002-induced apoptosis
• sympathetic neurons are partially protected from apoptosis induced by the withdrawal of nerve growth factor
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• about 10-fold more T cells cultured without cytokines are alive after six days compared to controls
(J:73316)
• double positive T cells with increased resistance to the apoptotic effects of dexamethasone
(J:133084)
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• sympathetic neurons are partially protected from apoptosis induced by the withdrawal of nerve growth factor
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• increased spleen weight (>2.5 fold)
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• irradiated mice reconstituted with mutant fetal liver cells have a 2- to 4- fold increase in lymphocyte and myeloid cell numbers compared to controls
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• increased white blood cells numbers (>3 fold)
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• at 6 months
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• T1 transitional B cells are insignificantly elevated
• increased T1 transitional B cells in inguinal lymph nodes
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• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells
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• mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to wild-type cells
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• about 10-fold more T cells cultured without cytokines are alive after six days compared to controls
(J:73316)
• double positive T cells with increased resistance to the apoptotic effects of dexamethasone
(J:133084)
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• increased spleen weight (>2.5 fold)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• increased number of splenocytes (>3 fold)
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• increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• increased number of splenocytes (>3 fold)
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• increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• ex vivo upon cytokine depletion compared with wild-type
• in response to cis-diammine-dichloroplatinum II (cisplatin), phorbol 12- myristate 13-acetate (PMA), dexamethasone (Dex), anti-Fas, and UV irradiation
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type
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• 2- to 4- fold increase in B220+ positive cells
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• 2- to 4- fold increase
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• increase in the number of HSV-1 specific T cells in the spleen but not lymph nodes after infection
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• HSV-1 antigen specific CD8+ T cells show nearly complete resistance to cytokine withdrawal induced apoptosis in culture
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• delay in postlactational remodeling of the mammary gland
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• ex vivo upon cytokine depletion compared with wild-type
• in response to cis-diammine-dichloroplatinum II (cisplatin), phorbol 12- myristate 13-acetate (PMA), dexamethasone (Dex), anti-Fas, and UV irradiation
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type
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• ex vivo upon cytokine depletion compared with wild-type
• in response to cis-diammine-dichloroplatinum II (cisplatin), phorbol 12- myristate 13-acetate (PMA), dexamethasone (Dex), anti-Fas, and UV irradiation
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• delay in postlactational remodeling of the mammary gland
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| N |
• unlike in Bnip3ltm1Ney homozygotes, reticulocytes clearance of mitochondria is normal
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• ex vivo upon cytokine depletion compared with wild-type
• in response to cis-diammine-dichloroplatinum II (cisplatin), phorbol 12- myristate 13-acetate (PMA), dexamethasone (Dex), anti-Fas, and UV irradiation
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type
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• 2- to 4- fold increase in B220+ positive cells
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• 2- to 4- fold increase
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• increase in the number of HSV-1 specific T cells in the spleen but not lymph nodes after infection
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• HSV-1 antigen specific CD8+ T cells show nearly complete resistance to cytokine withdrawal induced apoptosis in culture
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by 1 year of age 55% of homozygotes are terminally ill with 85% of these developing kidney disease (immune complex glomerulonephritis)
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• Background Sensitivity: about half die before E10; however, this percentage is highly variable and background dependent
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• in adult mice, thymic pre-T cells are reduced by about 50% compared to wild-type; however in newborns thymocyte composition is similar to wild-type
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• blood, spleen, and lymph node leukocyte numbers are elevated several fold mostly as a result of increased B and T cell counts
• these cells are mostly small, noncycling cells suggesting an increase in cell survival rather than proliferation
• however, the number of red cells is normal and the number of hematopoietic progenitor cells in the bone marrow is similar to wild-type
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• 2- to 4-fold increase
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• 2- to 4-fold increase
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• plasmablast numbers in spleen are increased relative to wild-type
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• higher numbers of T1 and T2 B cells in spleen relative to wild-type
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• higher in spleen relative to wild-type and Faslpr homozygotes
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• higher in spleen relative to wild-type and Faslpr homozygotes
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• 2- to 4-fold increase involving mostly mature T cells is seen in adult mice
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• 2- to 3-fold increase is seen in adult mice
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• 2- to 3-fold increase in the number of CD4+ T cells is seen in adult mice
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• 2- to 3-fold increase in the number of CD8+ T cells is seen in adult mice
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• 2- to 4-fold increase
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• at 6 to 12 months of age the number of IgM and IgG is increased by about 4-fold and 30- to 200-fold, respectively
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• in older homozygotes spleen size is increased 5- to 10-fold
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• seen in older homozygotes with plasma cells most severely affected
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• T1:follicular B cell ratio is higher than wild-type
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• total number of CD19+ splenocytes is higher than wild-type
• total number of splenocytes is increased relative to wild-type
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• pre-T cells cultured without cytokines or with ionomycin or taxol survive 10 to 30 times better than wild-type cells
• pre-T cells are resistant to dexamethasone or gamma-irradiation induced cell death but not to phorbol 12-myristate 13-acetate (PMA) or Fas ligand induced cell death
• pre-B cells are also resistant to cell death induced by cytokine withdrawal, ionomycin, taxol, and dexamethasone but display sensitivity to cell death similar to wild-type following gamma irradiation or exposure to PMA or Fas ligand
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• IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice
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• anti-CD40 B cell proliferation is increased 3- to 5- fold in the presence of IL-21, which does not occur in wild-type controls
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• elevated about 3-fold at 6 to 12 months of age
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• elevated about 10-fold at 6 to 12 months of age
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• elevated about 3-fold at 6 to 12 months of age
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• older homozygotes develop lymphadenopathy
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• total anti-IgM antibody levels are increased compared to wild-type
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• anti-nuclear antibodies are increased compared to wild-type
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• anti-SsDNA IgM and IgG antibodies are increased compared to wild-type
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• increased compared to wild-type
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• anti-ssDNA IgM and IgG antibodies are increased compared to wild-type
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• by 1 year of about 85% of terminally ill homozygotes have immune complex glomerulonephritis
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• higher numbers of apoptotic cells are detected in glomeruli compared to wild-type
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• number of macrophages surrounding glomeruli is increased compared to wild-type and Faslpr homozygotes
• number of macrophages surrounding glomeruli is increased compared to wild-type, and is similar to that in double mutants
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• IgG deposits mainly localized to glomerular basement membrane are increased relative to wild-type
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• hypercellularity is seen in homozygotes with kidney disease
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• by 1 year of about 85% of terminally ill homozygotes have immune complex glomerulonephritis
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• proliferation of capsular epithelial cells is seen in homozygotes with kidney disease
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• eosinophilic deposits are seen in dilated renal tubules in homozygotes with kidney disease
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• dilated renal tubules contain eosinophilic casts
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| N |
• unlike mice homozygous for Bbc3tm1Ast neuron sensitivity to oxidative stressor induced apoptosis is similar to controls
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• in adult mice, thymic pre-T cells are reduced by about 50% compared to wild-type; however in newborns thymocyte composition is similar to wild-type
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• platelet count is reduced to about 1/2 of wild-type; however, megakaryocyte numbers are normal
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• blood, spleen, and lymph node leukocyte numbers are elevated several fold mostly as a result of increased B and T cell counts
• these cells are mostly small, noncycling cells suggesting an increase in cell survival rather than proliferation
• however, the number of red cells is normal and the number of hematopoietic progenitor cells in the bone marrow is similar to wild-type
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• 2- to 4-fold increase
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• 2- to 4-fold increase
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• plasmablast numbers in spleen are increased relative to wild-type
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• higher numbers of T1 and T2 B cells in spleen relative to wild-type
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• higher in spleen relative to wild-type and Faslpr homozygotes
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• higher in spleen relative to wild-type and Faslpr homozygotes
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• 2- to 4-fold increase involving mostly mature T cells is seen in adult mice
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• 2- to 3-fold increase is seen in adult mice
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• 2- to 3-fold increase in the number of CD4+ T cells is seen in adult mice
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• 2- to 3-fold increase in the number of CD8+ T cells is seen in adult mice
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• 2- to 4-fold increase
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• at 6 to 12 months of age the number of IgM and IgG is increased by about 4-fold and 30- to 200-fold, respectively
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• in older homozygotes spleen size is increased 5- to 10-fold
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• seen in older homozygotes with plasma cells most severely affected
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• T1:follicular B cell ratio is higher than wild-type
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• total number of CD19+ splenocytes is higher than wild-type
• total number of splenocytes is increased relative to wild-type
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• pre-T cells cultured without cytokines or with ionomycin or taxol survive 10 to 30 times better than wild-type cells
• pre-T cells are resistant to dexamethasone or gamma-irradiation induced cell death but not to phorbol 12-myristate 13-acetate (PMA) or Fas ligand induced cell death
• pre-B cells are also resistant to cell death induced by cytokine withdrawal, ionomycin, taxol, and dexamethasone but display sensitivity to cell death similar to wild-type following gamma irradiation or exposure to PMA or Fas ligand
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• IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice
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• anti-CD40 B cell proliferation is increased 3- to 5- fold in the presence of IL-21, which does not occur in wild-type controls
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• elevated about 3-fold at 6 to 12 months of age
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• elevated about 10-fold at 6 to 12 months of age
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• elevated about 3-fold at 6 to 12 months of age
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• about 20% of terminally ill homozygotes show signs of vasculitis or cardiac infarction
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• IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice
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• higher numbers of apoptotic cells are detected in glomeruli compared to wild-type
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• anti-CD40 B cell proliferation is increased 3- to 5- fold in the presence of IL-21, which does not occur in wild-type controls
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• in older homozygotes spleen size is increased 5- to 10-fold
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• seen in older homozygotes with plasma cells most severely affected
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increase in spleen weight (<2 fold)
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• increased white blood cells numbers (<1.5 fold)
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| N |
• normal in vitro survival assays with several cytotoxic stimuli on sorted thymocyte and B cell populations
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• increase in spleen weight (<2 fold)
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• increased white blood cells numbers (<1.5 fold)
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• increase in spleen weight (<2 fold)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by 1 year of age 35% of heterozygotes are terminally ill
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• pre-T cells cultured without cytokines or with ionomycin or taxol survive better than wild-type cells but not as well as homozygous cells
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• increased at 6 to 12 months of age but less than in homozygous mice
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• increased at 6 to 12 months of age but less than in homozygous mice
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• increased at 6 to 12 months of age but less than in homozygous mice
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• older heterozygotes develop lymphadenopathy
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• by 1 year of about 85% of terminally ill heterozygotes have immune complex glomerulonephritis
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• by 1 year of about 85% of terminally ill heterozygotes have immune complex glomerulonephritis
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• about 20% of terminally ill heterozygotes show signs of vasculitis or cardiac infarction
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• pre-T cells cultured without cytokines or with ionomycin or taxol survive better than wild-type cells but not as well as homozygous cells
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• increased at 6 to 12 months of age but less than in homozygous mice
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• increased at 6 to 12 months of age but less than in homozygous mice
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• increased at 6 to 12 months of age but less than in homozygous mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 20 to 28 weeks of age, mice exhibit increased germinal center B cell number compared to controls but not as much as in Gt(ROSA)26Sortm3Rsky/Gt(ROSA)26Sortm3Rsky/ Tg(CD2-cre)1Kio mice
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• at 20 to 28 weeks of age, mice accumulate more antigen-experienced T cells than in control mice but not as much as in Gt(ROSA)26Sortm3Rsky/Gt(ROSA)26Sortm3Rsky/ Tg(CD2-cre)1Kio mice
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• at 20 to 28 weeks of age, mice exhibit increased germinal center B cell number compared to controls but not as much as in Gt(ROSA)26Sortm3Rsky/Gt(ROSA)26Sortm3Rsky/ Tg(CD2-cre)1Kio mice
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• at 20 to 28 weeks of age, mice accumulate more antigen-experienced T cells than in control mice but not as much as in Gt(ROSA)26Sortm3Rsky/Gt(ROSA)26Sortm3Rsky/ Tg(CD2-cre)1Kio mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Bcl2l11tm1.1Ast/Bcl2l11+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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• while B cell numbers are increased relative to in Cd79atm1(cre)Reth/Cd79a+ Bcl2l11tm1.1Ast/Bcl2l11+ Dicer1tm1Mmk/Dicer1tm1Mmk mice, numbers are still lower than in wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• B cell development is rescued compared with Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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• compared with control and Atmintm1.2Jhh/Atmintm1.2Jhh Cd79atm1(cre)Reth/Cd79a+ mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• arrests before the completion of meiosis in the preleptotene spermatocyte stage identical to Baxtm1Sjk homozygotes
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• the epithelium is grossly dysmorphic
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• tubule diameter is decreased and no spermatids are seen
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• males but not females are sterile
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• the epithelium is grossly dysmorphic
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• tubule diameter is decreased and no spermatids are seen
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• arrests before the completion of meiosis in the preleptotene spermatocyte stage identical to Baxtm1Sjk homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice are healthy through at least 6 months of age
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• coat color becomes gray at 5 to 7 weeks of age during completion of the second hair follicle cycle
• from the second follicle cycle on all newly grown hairs lack melanin and when an area is shaved the coat appears white suggesting the gray color is a result of unshed hairs from the first cycle
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• by 5 to 7 weeks of age melanocytes are lost from the hair follicles
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• absent from hair starting at the second hair follicle cycle
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• coat colors become grey after two hair follicle cycles
• in areas where skin is shaved, hair comes back completely white
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| N |
• ear length is normal
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to cells from mice homozygous for Bcl2tm1Dlo alone
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• culturing with IL-7 only partially rescues T cells from apoptosis
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| N |
• mice do not develop polycystic kidney disease
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• coat color becomes gray at 5 to 7 weeks of age during completion of the second hair follicle cycle
• from the second follicle cycle on all newly grown hairs lack melanin and when an area is shaved the coat appears white suggesting the gray color is a result of unshed hairs from the first cycle
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• by 5 to 7 weeks of age melanocytes are lost from the hair follicles
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• absent from hair starting at the second hair follicle cycle
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• coat colors become grey after two hair follicle cycles
• in areas where skin is shaved, hair comes back completely white
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• culturing with IL-7 only partially rescues T cells from apoptosis
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to cells from mice homozygous for Bcl2tm1Dlo alone
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• culturing with IL-7 only partially rescues T cells from apoptosis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice are healthy through at least 6 months of age
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| N |
• ear length is normal
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• irradiated mice reconstituted with mutant fetal liver cells have an increase in lymphocyte and myeloid cell numbers compared to controls
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to cells from mice homozygous for Bcl2tm1Dlo alone and cells from wild-type mice
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• culturing with IL-7 only partially rescues T cells from apoptosis over a 6 day period
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| N |
• mice do not develop polycystic kidney disease
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• irradiated mice reconstituted with mutant fetal liver cells have an increase in lymphocyte and myeloid cell numbers compared to controls
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• survival of mature resting T and B cells in the absence of cytokines is improved compared to cells from mice homozygous for Bcl2tm1Dlo alone and cells from wild-type mice
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• culturing with IL-7 only partially rescues T cells from apoptosis over a 6 day period
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| N |
• coat color is normal
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• about 10-fold more B cells cultured without cytokines are alive after six days compared to controls
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• culturing with IL-7 only partially rescues T cells from apoptosis over a 6 day period
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• reduced number of homozygous mice generated from intercross of the double heterozygous compared with the expected Mendelian inheritance
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• decreased body weight at 6 weeks old
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• persistence of interdigital tissue on both the front and rear paws
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• imperforate vagina in female mice
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• hydrometrocolpos in female mice
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• increased viability of CD4-positive and CD8-positive T cells during in vitro culture of T cells purified from spleen and lymph nodes
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• increased numbers of CD4-positive, CD8-positive, and CD4/CD8 double-positive thymocytes
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• a larger increased number of splenocytes (>5 fold)
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• increased viability of CD4-positive and CD8-positive T cells during in vitro culture of T cells purified from spleen and lymph nodes
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• increased numbers of CD4-positive, CD8-positive, and CD4/CD8 double-positive thymocytes
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• a larger increased number of splenocytes (>5 fold)
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• increased viability of CD4-positive and CD8-positive T cells during in vitro culture of T cells purified from spleen and lymph nodes
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• increased numbers of CD4-positive, CD8-positive, and CD4/CD8 double-positive thymocytes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• increased numbers of splenocytes (about 2.5 fold) compared with the wild type
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• a large increase in the number of B cells in the spleen
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• a large increase in the number of CD4-positive T cells in the spleen
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• a large increase in the number of CD8-positive T cells in the spleen
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• increased numbers of splenocytes (about 2.5 fold) compared with the wild type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• seen in about 50% of mice
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• increase in size is similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• variable abnormalities are seen including vacuolation, multi-nucleate syncytia, accumulation of spermatocytes, and apparent reduction in tubular cellularity, diameter, and lumen size
• the spermatogonia layer is thicker than in wild-type being up to 3 cell layers thick
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• testes weight is reduced at P21 and in adults testes weigh about 60% that of wild-type; however serum concentrations of inhibin and follicle stimulating hormone are normal
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• at P15 the number of germ cells in the testis is increased and these are mostly c-Kit+ early germ cells, but in adult males the number of germ cells is decreased by up to 10-fold compared to wild-type
• in adult males the proportion of early germ cells is increased up to 10-fold compared to controls; however the absolute number is similar to controls
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• arrest is later than in Baxtm1Sjk homozygotes as some round spermatids are present but no elongating spermatids are seen
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• males but not females are sterile
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• increase in size is similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in the bone marrow the pro/pre-B and immature B cell populations are reduced and the population of mature b cells is increased similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• in bone marrow
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• abnormalities in T cell development in the thymus (decrease in pre-T cells and increase in pro- and mature T cells) are identical to those in mice homozygous for Bcl2l11tm1.1Ast alone
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• similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• increase in mature B cells in the spleen and bone marrow similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• increase in mature T cells in the spleen similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• survival of mature T cells is increased following cytokine withdrawal or treatment with ionomycin similar to responses in mice homozygous for Bcl2l11tm1.1Ast alone
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• arrest is later than in Baxtm1Sjk homozygotes as some round spermatids are present but no elongating spermatids are seen
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• increase in size is similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in the bone marrow the pro/pre-B and immature B cell populations are reduced and the population of mature b cells is increased similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• in bone marrow
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• abnormalities in T cell development in the thymus (decrease in pre-T cells and increase in pro- and mature T cells) are identical to those in mice homozygous for Bcl2l11tm1.1Ast alone
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• similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• increase in mature B cells in the spleen and bone marrow similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• in bone marrow
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• increase in mature T cells in the spleen similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• similar to mice homozygous for Bcl2l11tm1.1Ast alone
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• survival of mature T cells is increased following cytokine withdrawal or treatment with ionomycin similar to responses in mice homozygous for Bcl2l11tm1.1Ast alone
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• variable abnormalities are seen including vacuolation, multi-nucleate syncytia, accumulation of spermatocytes, and apparent reduction in tubular cellularity, diameter, and lumen size
• the spermatogonia layer is thicker than in wild-type being up to 3 cell layers thick
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• testes weight is reduced at P21 and in adults testes weigh about 60% that of wild-type; however serum concentrations of inhibin and follicle stimulating hormone are normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• severely enlarged by 16 weeks
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• increased in spleen relative to Faslpr homozygotes
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• lower in spleen and lymph node compared to wild-type or single mutants
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• mice show reduced amount of nae T cells compared to wild-type or Bcl2l11-deficient mice
• no difference in number of regulatory T cells is observed
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• T cell subpopulations in the spleen and lymph nodes including single-positive, central memory and effector memory T cells are increased compared to single-deficient mice
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• increased memory T cell numbers in spleen and lymph nodes relative to single mutant animals
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• number of CD45+ cells is increased 4.6-fold vs wild-type, 3.2-fold vs Faslpr homozygotes, and 2.8-fold vs Bcl2l11-deficient mice
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• plasmablast numbers in spleen are increased 30-fold relative to wild-type, 2-fold relative to Bcl2l11-deficient and 4-fold relative to Faslpr homozygous mice
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• number of T1 and T2 B cells is increased relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• higher in spleen relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• higher in spleen relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• increased numbers in spleen and lymph nodes are observed
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• increased effector memory T cell numbers in spleen and lymph nodes relative to single mutant animals
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• enlargement of spleen is accompanied by alteration of spleen architecture, characterized by decreased red pulp amount
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• enlargement of spleen is accompanied by alteration of spleen architecture, characterized by increased white pulp amount
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• T1:follicular B cell ratio is disturbed; ratio is higher significantly higher than wild-type, Bcl2l11-deficient mice or Faslpr homozygotes
• increased B cell number; CD19+ B cells are increased by 4.3-fold over wild-type, 1.8-fold over Bcl2l11-null mice and by 3.6-fold over Faslpr mice
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• CD19+ B cells are increased 2.2-fold over wild-type and Bcl2l11-deficient mice
• no difference from B cell number in Faslpr mice
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• severely enlarged by 16 weeks
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• on a normally resistant background, mice develop extreme lymphadenopathy and SLE
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• total anti-IgM and total anti-IgG antibody levels are increased compared to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice; anti-nuclear, anti-cytoplasmic and anti-glomerular antibodies are increased relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• anti-nuclear antibodies are increased compared to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• anti-dsDNA IgM and IgG antibodies are increased compared to wild-type and Bcl2l11-deficient mice; anti-dsDNA IgM antibody levels are increased over levels in Faslpr homozygotes
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• increased compared to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• anti-ssDNA IgM and IgG antibodies are increased compared to wild-type and Bcl2l11-deficient mice; anti-ssDNA IgM antibody levels are increased over levels in Faslpr homozygotes
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• severely enlarged by 16 weeks
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• increased in spleen relative to Faslpr homozygotes
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• lower in spleen and lymph node compared to wild-type or single mutants
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|
• mice show reduced amount of nae T cells compared to wild-type or Bcl2l11-deficient mice
• no difference in number of regulatory T cells is observed
|
|
• T cell subpopulations in the spleen and lymph nodes including single-positive, central memory and effector memory T cells are increased compared to single-deficient mice
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|
• increased memory T cell numbers in spleen and lymph nodes relative to single mutant animals
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|
• number of CD45+ cells is increased 4.6-fold vs wild-type, 3.2-fold vs Faslpr homozygotes, and 2.8-fold vs Bcl2l11-deficient mice
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|
• plasmablast numbers in spleen are increased 30-fold relative to wild-type, 2-fold relative to Bcl2l11-deficient and 4-fold relative to Faslpr homozygous mice
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|
• number of T1 and T2 B cells is increased relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• higher in spleen relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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|
• higher in spleen relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
|
|
• increased numbers in spleen and lymph nodes are observed
|
|
• increased effector memory T cell numbers in spleen and lymph nodes relative to single mutant animals
|
|
• enlargement of spleen is accompanied by alteration of spleen architecture, characterized by decreased red pulp amount
|
|
• enlargement of spleen is accompanied by alteration of spleen architecture, characterized by increased white pulp amount
|
|
• T1:follicular B cell ratio is disturbed; ratio is higher significantly higher than wild-type, Bcl2l11-deficient mice or Faslpr homozygotes
• increased B cell number; CD19+ B cells are increased by 4.3-fold over wild-type, 1.8-fold over Bcl2l11-null mice and by 3.6-fold over Faslpr mice
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• higher numbers of apoptotic cells are detected in glomeruli compared to wild-type and single mutant mice
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• glomerular proliferation is increased
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• significant increase in renal B cells (CD19+) compared to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
• number of macrophages surrounding glomeruli is increased compared to wild-type and Faslpr homozygotes; macrophage index is 2.5-fold higher than in Fas homozygotes
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• IgG deposits mainly localized to basement glomerular membrane are increased relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• glomerular basement membrane thickening
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• mesangial expansion, basement membrane thickening, increased interstitial infiltration, and loss of open capillary loops are characteristic hallmarks of renal damage observed
• kidney damage pathological scores are increased over wild-type, Faslpr homozygotes, and Bcl2l11-deficient glomeruli
• glomerular proliferation is increased
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• loss of open capillary loops
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• mesangial expansion
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• glomerular size is increased relative to wild-type, Faslpr homozygotes, and Bcl2l11-deficient mice
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• higher numbers of apoptotic cells are detected in glomeruli compared to wild-type and single mutant mice
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• glomerular proliferation is increased
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• loss of open capillary loops
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• severely enlarged by 16 weeks
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• spleens are much heavier than those in homozygous Bcl-2 deficient mice
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• white blood cells numbers are much higher than in homozygous Bcl-2 deficient mice
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• spleens are much heavier than those in homozygous Bcl-2 deficient mice
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• white blood cells numbers are much higher than in homozygous Bcl-2 deficient mice
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• spleens are much heavier than those in homozygous Bcl-2 deficient mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• spleens are heavier than those in homozygous Bcl-2 deficient mice
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• more white blood cells numbers than in homozygous Bcl-2 deficient mice
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• spleens are heavier than those in homozygous Bcl-2 deficient mice
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• more white blood cells numbers than in homozygous Bcl-2 deficient mice
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• spleens are heavier than those in homozygous Bcl-2 deficient mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• colony forming erythroid cells in the bone marrow
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• T cells fail to develop
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• T cells fail to develop
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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• almost no loss of cellular viability ex vivo upon cytokine depletion compared with wild-type and Bcl2l11tm1.1Ast homozygous cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells and Bcl2l11tm1.1Ast homozygotes
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• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells and Bcl2l11tm1.1Ast homozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in the thymus, spleen and liver of mice treated with alpha- galactosylceramide CD1d1 tetramer
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• in the thymus, spleen and liver of mice treated with alpha- galactosylceramide CD1d1 tetramer
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• middle-aged mice exhibit a rescue of regulatory T cells compared with Il15tm1Imx homozygotes
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 06/12/2024 MGI 6.13 |
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