Phenotypes associated with this allele

• Allele Symbol: Bcl6^tm1Htno
• Allele Name: targeted mutation 1, Masahiko Hatano
• Allele ID: MGI:2158436
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bcl6^tm1Htno/Bcl6^tm1Htno	involves: 129S1/Sv * 129X1/SvJ	MGI:2664912
hm2	Bcl6^tm1Htno/Bcl6^tm1Htno	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:2664910
ht3	Bcl6^tm1Htno/Bcl6^+	involves: 129S1/Sv * 129X1/SvJ	MGI:2664913

Genotype
MGI:2664912

hm1

• Allelic Composition: Bcl6^tm1Htno/Bcl6^tm1Htno
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

oligozoospermia ( J:82037 )

♂ • reduced amount of epididymal sperm

increased male germ cell apoptosis ( J:82037 )

♂ • increase in germ cell apoptosis beginning at 8 weeks of age

♂ • apoptotic spermatocytes at metaphase I

♂ • apoptosis putatively due to increased susceptibility to stressors

reduced male fertility ( J:82037 )

♂ • 85% of males were unable to sire offspring when mated with wild-type females

decreased fertilization frequency ( J:82037 )

♂ • an impaired fertilization rate was shown in vitro

cellular

oligozoospermia ( J:82037 )

♂ • reduced amount of epididymal sperm

increased male germ cell apoptosis ( J:82037 )

♂ • apoptotic spermatocytes at metaphase I

♂ • increase in germ cell apoptosis beginning at 8 weeks of age

♂ • apoptosis putatively due to increased susceptibility to stressors

Genotype
MGI:2664910

hm2

• Allelic Composition: Bcl6^tm1Htno/Bcl6^tm1Htno
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

mortality/aging

premature death ( J:55952 )

• mutants become sick 4-6 weeks after birth and most die by 9 weeks after birth

postnatal lethality, incomplete penetrance ( J:55952 )

• number of mutants living longer than 3 weeks is smaller than expected

growth/size/body

decreased body size ( J:55952 )

• observed first around 3 weeks of age

decreased body weight ( J:55952 )

• mean body weight is less than controls at 3 weeks of age

cardiovascular system

myocardial fiber degeneration ( J:55952 )

• severe degeneration of myofibers is seen at necropsy

• prior to eosinophilic infiltration (at 4 weeks of age), myocytes already show degenerative changes such as irregular size of condensed mitochondria, indicating myoctes are damaged prior to eosinophilic infiltration

thin ventricular wall ( J:55952 )

dilated heart ventricle ( J:55952 )

cardiac interstitial fibrosis ( J:55952 )

myocarditis ( J:55952 )

• massive myocarditis with eosinophilic infiltration is seen after 4-6 weeks of age

immune system

myocarditis ( J:55952 )

• massive myocarditis with eosinophilic infiltration is seen after 4-6 weeks of age

abnormal spleen morphology ( J:55952 )

• inflammation with eosinophilic infiltration is seen in the spleen

lung inflammation ( J:55952 )

• inflammation with eosinophilic infiltration is seen in lungs

hematopoietic system

abnormal spleen morphology ( J:55952 )

• inflammation with eosinophilic infiltration is seen in the spleen

muscle

myocardial fiber degeneration ( J:55952 )

• severe degeneration of myofibers is seen at necropsy

• prior to eosinophilic infiltration (at 4 weeks of age), myocytes already show degenerative changes such as irregular size of condensed mitochondria, indicating myoctes are damaged prior to eosinophilic infiltration

respiratory system

lung inflammation ( J:55952 )

• inflammation with eosinophilic infiltration is seen in lungs

cellular

cardiac interstitial fibrosis ( J:55952 )

Genotype
MGI:2664913

ht3

• Allelic Composition: Bcl6^tm1Htno/Bcl6⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

cellular

abnormal apoptosis ( J:82037 )

• increased germ cell apoptosis