Phenotypes associated with this allele

• Allele Symbol: Bcl3^tm1Sbn
• Allele Name: targeted mutation 1, Ulrich Siebenlist
• Allele ID: MGI:2158443
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bcl3^tm1Sbn/Bcl3^tm1Sbn	involves: 129S4/SvJae	MGI:5574307
hm2	Bcl3^tm1Sbn/Bcl3^tm1Sbn	involves: 129S4/SvJae * C57BL/6	MGI:4352689

Genotype
MGI:5574307

hm1

• Allelic Composition: Bcl3^tm1Sbn/Bcl3^tm1Sbn
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased transitional stage T1 B cell number ( J:207111 )

decreased transitional stage T2 B cell number ( J:207111 )

decreased follicular B cell number ( J:207111 )

increased marginal zone B cell number ( J:207111 )

• intrinsic to B cells and cell autonomous

abnormal B cell physiology ( J:207111 )

• in cocultures, splenic B cells result in better proliferation of CD4+ T cells compared with wild-type cells

decreased B cell proliferation ( J:207111 )

• of alphaIgM-stimulated follicular B cells

increased B cell proliferation ( J:207111 )

• of LPS-stimulated marginal zone and follicular B cells

hematopoietic system

decreased transitional stage T1 B cell number ( J:207111 )

decreased transitional stage T2 B cell number ( J:207111 )

decreased follicular B cell number ( J:207111 )

increased marginal zone B cell number ( J:207111 )

• intrinsic to B cells and cell autonomous

abnormal B cell physiology ( J:207111 )

• in cocultures, splenic B cells result in better proliferation of CD4+ T cells compared with wild-type cells

decreased B cell proliferation ( J:207111 )

• of alphaIgM-stimulated follicular B cells

increased B cell proliferation ( J:207111 )

• of LPS-stimulated marginal zone and follicular B cells

cellular

decreased B cell proliferation ( J:207111 )

• of alphaIgM-stimulated follicular B cells

increased B cell proliferation ( J:207111 )

• of LPS-stimulated marginal zone and follicular B cells

Genotype
MGI:4352689

hm2

• Allelic Composition: Bcl3^tm1Sbn/Bcl3^tm1Sbn
• Genetic Background: involves: 129S4/SvJae * C57BL/6

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:39747 )

• unlike in wild-type mice, infection with T. gondii leads to death within 3 to 5 weeks

immune system

decreased macrophage cell number ( J:39747 )

• mice exhibit fewer metallophillic macrophages and ER-TR9+ marginal zone macrophages compared with wild-type mice

abnormal lymphocyte morphology ( J:39747 )

• the ratios of B to T cells in the spleen, blood, and lymph nodes are decreased compared to in wild-type mice

abnormal B cell differentiation ( J:39747 )

• unchallenged mice exhibit smaller B cell compartments compared to in wild-type mice

abnormal class switch recombination ( J:39747 )

• impaired following infection with influenza virus

decreased B cell number ( J:39747 )

• mice challenged with influenze or T. gondii exhbit a progressive loss of B cells from secondary lymphoid organs unlike in similarly treated wild-type mice

abnormal metallophilic macrophage morphology ( J:39747 )

• mice exhibit fewer metallophillic macrophages that are no longer organized in a continuous ring of cells as in wild-type mice

abnormal spleen marginal zone macrophage morphology ( J:39747 )

• no ER-TR9+ marginal zone macrophages are detected unlike in wild-type mice

intermingled spleen red and white pulp ( J:39747 )

• red pulp contains infiltrates from the white pulp unlike in wild-type mice

absent spleen germinal center ( J:39747 )

• in unchallenged mice and mice challenged with influenze virus or T. gondii fail to exhibit splenic germinal centers unlike similarly treated wild-type mice

decreased spleen germinal center size ( J:39747 )

• in mice challenged with TNP-KLH fewer PNA+ germinal centers are formed compared to in similarly treated wild-type mice

disorganized spleen B cell follicle ( J:39747 )

• spleen B cell follicles lack distinct organization unlike in wild-type mice

decreased IgG2a level ( J:39747 )

• following infection with influenza virus

abnormal cytotoxic T cell physiology ( J:39747 )

• nitric oxide production of T cells in response to STAg is reduced compared to in similarly treated wild-type cells

abnormal lymph node B cell domain morphology ( J:39747 )

• lymph node B cell follicles lack distinct organization unlike in wild-type mice

lymph node hypoplasia ( J:39747 )

• variable

decreased interferon-gamma secretion ( J:39747 )

• soluble tachyzoite antigen (STAg)-treated splenocyted exhibit a rapid decline in IFN-gamma production compared with similarly treated wild-type cells

• T cell production of IFN-gamma following exposure to STAg is impaired while that of NK cells is normal

• however, T cell IFN-gamma production in response to anti-CD3 treatment is normal

decreased interleukin-12 secretion ( J:39747 )

• in STAg-treated T cells

increased susceptibility to parasitic infection ( J:39747 )

• unlike in wild-type mice, infection with T. gondii leads to inflammation in mulitpe tissues, thymus necrosis, secondary lymphoid tissue acellularity, loss of T cells from secondary lymphoid organs, and death within 3 to 5 weeks

• soluble tachyzoite antigen (STAg)-treated splenocyted exhibit a rapid decline in IFN-gamma production compared with similarly treated wild-type cells

• T cell production of IFN-gamma following exposure to STAg is impaired while that of NK cells is normal

• nitric oxide and IL12 production of T cells in response to STAg is reduced compared to in similarly treated wild-type cells

• cytotoxic T lymphocyte activity towards T. gondii is impaired compared to in similarly treated wild-type mice

• mice fail to mount a protective T helper 1 immune resposne against T. gondii infection unlike similarly treated wild-type mice

homeostasis/metabolism

abnormal nitric oxide homeostasis ( J:39747 )

• nitric oxide production of T cells in response to STAg is reduced compared to in similarly treated wild-type cells

hematopoietic system

decreased macrophage cell number ( J:39747 )

• mice exhibit fewer metallophillic macrophages and ER-TR9+ marginal zone macrophages compared with wild-type mice

abnormal lymphocyte morphology ( J:39747 )

• the ratios of B to T cells in the spleen, blood, and lymph nodes are decreased compared to in wild-type mice

abnormal B cell differentiation ( J:39747 )

• unchallenged mice exhibit smaller B cell compartments compared to in wild-type mice

abnormal class switch recombination ( J:39747 )

• impaired following infection with influenza virus

decreased B cell number ( J:39747 )

• mice challenged with influenze or T. gondii exhbit a progressive loss of B cells from secondary lymphoid organs unlike in similarly treated wild-type mice

abnormal metallophilic macrophage morphology ( J:39747 )

• mice exhibit fewer metallophillic macrophages that are no longer organized in a continuous ring of cells as in wild-type mice

abnormal spleen marginal zone macrophage morphology ( J:39747 )

• no ER-TR9+ marginal zone macrophages are detected unlike in wild-type mice

intermingled spleen red and white pulp ( J:39747 )

• red pulp contains infiltrates from the white pulp unlike in wild-type mice

absent spleen germinal center ( J:39747 )

• in unchallenged mice and mice challenged with influenze virus or T. gondii fail to exhibit splenic germinal centers unlike similarly treated wild-type mice

decreased spleen germinal center size ( J:39747 )

• in mice challenged with TNP-KLH fewer PNA+ germinal centers are formed compared to in similarly treated wild-type mice

disorganized spleen B cell follicle ( J:39747 )

• spleen B cell follicles lack distinct organization unlike in wild-type mice

decreased IgG2a level ( J:39747 )

• following infection with influenza virus

abnormal cytotoxic T cell physiology ( J:39747 )

• nitric oxide production of T cells in response to STAg is reduced compared to in similarly treated wild-type cells