Analysis Tools|
Allele Symbol Allele Name Allele ID |
Tg(Pou3f4-cre)32Cren transgene insertion 32, Bryan Crenshaw III MGI:2158470 |
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| Summary |
13 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased cell death without a decrease in cell proliferation
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• increased cell death without a decrease in cell proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• proliferation of cells declined by E10
• 45% at E11 to 50% at E11.5
• E11 proliferation rate down 35%
• 4.8X increase in progenitor cell death and apoptosis
• higher proportion of differentiated neurons to proliferative cells
• number of neurons unchanged
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• changes similar to those seen in the spinal cord
• tissue mass of the midbrain was reduced
• progenitor domains in the midbrain and other areas of the brain reduced
• increased apoptosis
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• ventricular zone absent at E12
• reduced area occupied by progenitor cells
• ventral progenitor are absent by E11.5
• absent dorsally by E12
• Larger area occupied by differentiated neurons(as determined immunohistochemically and gene markers)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at P7, numbers of Zfp276-positive oligodendrocytes in the spinal cord only reach about 7% of those in controls, as identified by Olig2 co-staining
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• despite the absence of visceral or branchial motoneurons, the main trunk of the facial nerve is present
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• visceromotor neurons are not produced
• absence of visceral or branchial motoneurons
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• survived up to 17 days
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• shafts usually fail to form in follicles
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• wavy
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• misshapen and expanded dermal papillae
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• misangled
• not as deep into the dermis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• ectopic distal phalanges are sometimes found
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• although reduced digits is typical, polydactyly sometimes occurs
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• polysyndactyly is common
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• if hindlimbs are present then they are highly malformed
• loss of ventral structures of hindlimbs
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• in many but not all animals
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• whole brain is smaller than wild-type brain at E18.5
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• normal distribution of interneurons in cerebral cortex is lost in mutants, with Calb1+ interneurons restricted to subcortical and subventricular regions
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• olfactory bulbs are nearly absent at E18.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased proliferation of cells by E10
• 110% at E11 to 300% at E11.5
• E11 proliferation rate increased 1.4X
• 6.7% increase in progenitor cell death and apoptosis
• lower proportion of differentiated neurons to proliferative cells
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• changes similar to those seen in the spinal cord
• tissue mass of the midbrain was increased
• increased proliferation in all areas of the brain
• progenitor domains of the midbrain and other areas of the brain increased
• increased apoptosis
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• enlarged ventricular zone at E10.5
• increased area occupied by neural progenitor cells
• smaller area occupied by differentiated neurons (as determined immunohistochemically and by activity of genes expressed in progenitor cells)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
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• almost complete loss of small diameter nociceptive fibers in the dorsal horn
• reduction in the innervation of TrkA+ and aquaporin 1+ sensory neurons in the dorsal horn
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• in dorsal spinal neurons neurites are severely reduced and remaining structures often appear misshapen
• cultured neurons from the superficial dorsal horn show a decrease in the overall number, length and branching frequency of neurites
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• almost complete loss of small diameter nociceptive fibers in the dorsal horn at E16.5
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• the superficial zone is invariably compressed and the nuclei appear compacted
• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
• in dorsal spinal neurons neurites are severely reduced and remaining structures often appear misshapen
• the density of DiI positive fibers is greatly reduced and the remaining fibers appear disorganized at E16.5
• almost complete loss of small diameter nociceptive fibers in the dorsal horn
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• the success rate for evoked currents is reduced in dorsal horn neurons
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• expression of late differentiation markers in dorsal spinal neurons is reduced indicating a defect in terminal differentiation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die at late embryonic stages
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| N |
• mice exhibit normal basolateral and radial fibers, and glia- and motor neuron generation
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• increased apoptosis in the spinal cord at E11.5 and E12.5
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• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells
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• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells
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• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice
• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice
• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice
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• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice
• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice
• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice
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• increased apoptosis in the spinal cord at E11.5 and E12.5
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• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells
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• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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