Phenotypes associated with this allele

• Allele Symbol: Bid^tm1Sjk
• Allele Name: targeted mutation 1, Stanley J Korsmeyer
• Allele ID: MGI:2158671
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bid^tm1Sjk/Bid^tm1Sjk	B6.129X1-Bid^tm1Sjk	MGI:3796608
hm2	Bid^tm1Sjk/Bid^tm1Sjk	involves: 129X1/Sv * C57BL/6	MGI:3796578
hm3	Bid^tm1Sjk/Bid^tm1Sjk	involves: 129X1/SvJ	MGI:2657300
hm4	Bid^tm1Sjk/Bid^tm1Sjk	involves: 129X1/SvJ * C57BL/6	MGI:3799149
cx5	Bid^tm1Sjk/Bid^tm1Sjk Cstb^tm1Rm/Cstb^tm1Rm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3796609
cx6	Bid^tm1Sjk/Bid^tm1Sjk Gdnf^tm1Rosl/Gdnf^+	involves: 129S2/SvPas * 129X1/SvJ * C57BL/6	MGI:3588577
cx7	Bid^tm1Sjk/Bid^tm1Sjk Rela^tm1Bal/Rela^tm1Bal	involves: 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:3796611

Genotype
MGI:3796608

hm1

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk
• Genetic Background: B6.129X1-Bid^tm1Sjk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal hippocampus morphology ( J:111236 )

• 2 days after neonatal hypoxia-ischemia (HI) injury, ispsilateral hippocampal area loss is not decreased relative to that observed in wild-type neonates

gliosis ( J:111236 )

• reactive gliosis is significantly decreased in hippocampi of mutants 2 days after neonatal HI compared to wild-type

respiratory system

respiratory system phenotype ( J:107667 )

N • after bleomycin treatment, mutant lungs show similar inflammatory response over same time course as observed for wild-type lungs

N • mouse alveolar epithelial cells show similar levels of cell death to controls after bleomycin treatment

N • lung fibroblasts show proliferation rates similar to wild-type under low serum conditions

abnormal pulmonary alveolus epithelial cell morphology ( J:107667 )

• no cell death is observed when cells are treated with Tgfb1, whereas cell death is induced in wild-type cells

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:107667 )

N • after bleomycin treatment, Tgfb1 levels in brochoalveolar lavage fluid are not different from control values

Genotype
MGI:3796578

hm2

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk
• Genetic Background: involves: 129X1/Sv * C57BL/6

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:120650 )

• when placed under hyperoxic conditions for more than 5 days, mice show increased survival (resistance to oxygen toxicity) compared to wild-type mice

respiratory system

abnormal lung morphology ( J:120650 )

• lung fibroblasts show increased resistance (less apoptosis) to hyperoxia than wild-type fibroblasts

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:116520 )

• treatment of islet cells with perforin/granzyme B does not result in cytochrome C release as seen in wild-type cells; release is blocked in Bid-null islets

• islet cells are resistant to granzyme B-mediated apoptosis, unlike wild-type cells

liver/biliary system

liver/biliary system phenotype ( J:132334 )

N • hepatocytes show normal sensitivity to Tnf-mediated hepatocyte destruction

Genotype
MGI:2657300

hm3

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk
• Genetic Background: involves: 129X1/SvJ

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:57073 )

• unlike wild-type mice that die from hepatocellular apoptosis and hemorrhagic necrosis after injection of an anti-Fas antibody, nearly all mutants survive

homeostasis/metabolism

decreased susceptibility to injury ( J:57073 )

• animals survive with little or no liver damage after injection with anti-Fas antibodies

cellular

decreased apoptosis ( J:57073 )

• mutants are resistant to Fas-induced hepatocellular apoptosis

abnormal mitochondrial physiology ( J:57073 )

• the transmembrane potential of mitochondria is better maintained in mutant MEFs treated with TNF-alpha or thymocytes treated with anti-Fas antibody than wild-type cells

Genotype
MGI:3799149

hm4

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk
• Genetic Background: involves: 129X1/SvJ * C57BL/6

immune system

decreased susceptibility to endotoxin shock ( J:117693 )

• LPS-GalN-induced liver injury, mice exhibit reduced liver injury and mortality during the early phase

• however, late phase response is normal and mice exhibit the same mortality as wild-type mice

Genotype
MGI:3796609

cx5

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk; Cstb^tm1Rm/Cstb^tm1Rm
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological

ataxia ( J:115694 )

• by 8 months of age, mice display ataxia; severity is similar to that of Cstb-single null mice

seizures ( J:115694 )

• 4occur during both sleep and wakefulness

myoclonus ( J:115694 )

• mice display frequent spontaneous axial myoclonic jerks at rate of 10-15/hour; head and neck are always involved, with whole body involved in most animals

nervous system

seizures ( J:115694 )

• 4occur during both sleep and wakefulness

myoclonus ( J:115694 )

• mice display frequent spontaneous axial myoclonic jerks at rate of 10-15/hour; head and neck are always involved, with whole body involved in most animals

abnormal cerebellar granule cell morphology ( J:115694 )

• widespread granule cell apoptosis is observed in mice at 2, 4, and 6 months of age, similar to Cstb-single null animals

abnormal brain wave pattern ( J:115694 )

• baseline cortical activity displays normal low-amplitude desynchronized EEG with intermittent interictal discharges and bilateral electrographic seizure discharges

vision/eye

abnormal cornea morphology ( J:115694 )

• at 8 months, around two-thirds of mutants may have serous exudate in one or both eyes, similar to Cstb-null mice

cornea scarring ( J:115694 )

• at 8 months, around two-thirds of mutants display a mild eye phenotype with corneal lesions, similar to Cstb-null mice

muscle

myoclonus ( J:115694 )

• mice display frequent spontaneous axial myoclonic jerks at rate of 10-15/hour; head and neck are always involved, with whole body involved in most animals

Genotype
MGI:3588577

cx6

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk; Gdnf^tm1Rosl/Gdnf⁺
• Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * C57BL/6

nervous system

abnormal enteric neuron morphology ( J:82456 )

• 32-33% and 43-48% reduction in submucosal and myenteric neurons, respectively, similar to that seen in single heterozygous Gdnf mutants

Genotype
MGI:3796611

cx7

• Allelic Composition: Bid^tm1Sjk/Bid^tm1Sjk; Rela^tm1Bal/Rela^tm1Bal
• Genetic Background: involves: 129S4/SvJae * 129X1/SvJ * C57BL/6

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:117693 )

• mice die around the same time as Rela^tm1Bal homozygotes: Bid deletion does not rescue the lethality

liver/biliary system

liver hemorrhage ( J:132334 )

• at E14.5 and 15.5, embryos show similar level of liver hemorrhaging as control Rela-null embryos

abnormal liver morphology ( J:132334 )

• at E14.5 and 15.5, embryos show similar level of liver destruction as control Rela-null embryos

cardiovascular system

liver hemorrhage ( J:132334 )

• at E14.5 and 15.5, embryos show similar level of liver hemorrhaging as control Rela-null embryos

cellular

decreased fibroblast apoptosis ( J:117693 )

• mouse embryonic fibroblasts are partially protected from TNF-alpha-induced apoptosis compared to exposed Rela^tm1Bal homozygous mouse embryonic fibroblasts