Phenotypes associated with this allele

• Allele Symbol: Camk4^tm1Arm
• Allele Name: targeted mutation 1, Anthony R Means
• Allele ID: MGI:2158694
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Camk4^tm1Arm/Camk4^tm1Arm	involves: 129 * C57BL/6J	MGI:2451150

Genotype
MGI:2451150

hm1

• Allelic Composition: Camk4^tm1Arm/Camk4^tm1Arm
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:66075 )

• less than 50% surviving into puberty

behavior/neurological

limb grasping ( J:70431 )

tremors ( J:70431 )

• moderate tremors are exhibited by 80% of the mutant mice

ataxia ( J:70431 )

impaired coordination ( J:70431 )

• shown in a vertical wire test and in a pole climbing test

decreased vertical activity ( J:70431 )

decreased locomotor activity ( J:70431 )

• spontaneous horizontal and vertical activity is reduced

reproductive system

abnormal reproductive system morphology ( J:66075 , J:70570 )

abnormal female reproductive system morphology ( J:66075 )

♀

absent corpus luteum ( J:66075 )

♀ • some corpora lutea were observed in subfertile females, however they retained oocytes or antral fluid

abnormal ovarian follicle morphology ( J:66075 )

♀ • some follicles contained multiple oocytes enclosed by a single layer of granulosa cells

♀ • with some exceptions, numerous preantral and preovulatory follicles were observed

polyovular ovarian follicle ( J:66075 )

♀ • some follicles contained multiple oocytes enclosed by a single layer of granulosa cells

abnormal gametogenesis ( J:70570 )

teratozoospermia ( J:70570 )

♂

abnormal sperm flagellum morphology ( J:70570 )

♂ • improper attachment of flagellum to sperm head

abnormal sperm head morphology ( J:70570 )

♂ • failure of sperm to develop hook-shaped head

♂ • improper attachment of flagellum to sperm head

♂ • degenerating sperm head

abnormal spermatid morphology ( J:70570 )

♂ • reduced number of step-15 spermatids

♂ • absence of step-16 spermatids

decreased male germ cell number ( J:70570 )

♂ • reduced number of step-15 spermatids

♂ • absence of step-16 spermatids

oligozoospermia ( J:70570 )

♂ • sperm counts were less than 4% of those of wild-type males

abnormal spermiogenesis ( J:70570 )

♂ • morphologically normal step-12 spermatids

♂ • reduced number of step-15 spermatids

♂ • absence of step-16 spermatids

arrest of spermatogenesis ( J:70570 )

♂

abnormal male reproductive system morphology ( J:70570 )

♂

seminiferous tubule degeneration ( J:70570 )

♂ • vacuoles, sloughing of germ cells and mulitnucleated giant cells are present

decreased testis weight ( J:70570 )

♂ • reduced testis weight; 33.7mg vs 110mg in controls

abnormal reproductive system physiology ( J:66075 )

anovulation ( J:66075 )

♀ • failure of ovulation, as indicated by an absence of or aberrant corpora lutea

♀ • no normal ovulation in response to supraphysiological doses of FSH and LH

reduced female fertility ( J:66075 )

♀ • females produced fewer litters

decreased litter size ( J:66075 )

• litter size reduced to 45% of those of wild-type females

male infertility ( J:70570 )

♂

nervous system

abnormal Purkinje cell morphology ( J:70431 )

• Purkinje cells exhibit immature morphological characteristics including multiple climbing fiber synapses

decreased Purkinje cell number ( J:70431 )

• reduced number of mature Purkinje cells

decreased excitatory postsynaptic current amplitude ( J:70431 )

• climbing fiber EPSCs have smaller amplitudes than controls

increased excitatory postsynaptic current amplitude ( J:70431 )

• parallel fiber EPSCs are more rapid and have larger amplitudes than controls

abnormal synaptic depression ( J:70431 )

• climbing fiber stimuli induce net facilitization followed by depression at longer stimulus intervals than controls

growth/size/body

decreased body weight ( J:70570 )

• average 19.7g vs 24.3g in controls

endocrine/exocrine glands

absent corpus luteum ( J:66075 )

♀ • some corpora lutea were observed in subfertile females, however they retained oocytes or antral fluid

abnormal ovarian follicle morphology ( J:66075 )

♀ • some follicles contained multiple oocytes enclosed by a single layer of granulosa cells

♀ • with some exceptions, numerous preantral and preovulatory follicles were observed

polyovular ovarian follicle ( J:66075 )

♀ • some follicles contained multiple oocytes enclosed by a single layer of granulosa cells

seminiferous tubule degeneration ( J:70570 )

♂ • vacuoles, sloughing of germ cells and mulitnucleated giant cells are present

decreased testis weight ( J:70570 )

♂ • reduced testis weight; 33.7mg vs 110mg in controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:70570 )

N • normal testosterone levels

cellular

teratozoospermia ( J:70570 )

♂

abnormal sperm flagellum morphology ( J:70570 )

♂ • improper attachment of flagellum to sperm head

abnormal sperm head morphology ( J:70570 )

♂ • failure of sperm to develop hook-shaped head

♂ • improper attachment of flagellum to sperm head

♂ • degenerating sperm head

abnormal spermatid morphology ( J:70570 )

♂ • reduced number of step-15 spermatids

♂ • absence of step-16 spermatids

decreased male germ cell number ( J:70570 )

♂ • reduced number of step-15 spermatids

♂ • absence of step-16 spermatids

oligozoospermia ( J:70570 )

♂ • sperm counts were less than 4% of those of wild-type males