Phenotypes associated with this allele

• Allele Symbol: Trio^tm1Mst
• Allele Name: targeted mutation 1, Michel Streuli
• Allele ID: MGI:2158695
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trio^tm1Mst/Trio^tm1Mst	involves: 129S4/SvJae * BALB/c	MGI:2655446

Genotype
MGI:2655446

hm1

• Allelic Composition: Trio^tm1Mst/Trio^tm1Mst
• Genetic Background: involves: 129S4/SvJae * BALB/c

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:65380 )

• ~40% of mutant embryos die between E18.5 and birth, or shortly after

lethality throughout fetal growth and development, incomplete penetrance ( J:65380 )

• ~60% of mutant embryos die between E15.5 and E18.5

muscle

abnormal myogenesis ( J:65380 )

• at E18.5, homozygotes exhibit absence of smaller secondary skeletal myofibers, suggesting a defect in secondary myogenesis which normally begins at ~E15

• however, in utero BrdU labeling indicates that secondary myoblast proliferation is normal at least through E15.5, suggesting a possible defect in the localized migration, alignment, or fusion of secondary myoblasts to form secondary myofibers

abnormal skeletal muscle morphology ( J:65380 )

• at E14.5, E16.5, and E18.5, homozygotes display abnormal skeletal muscle histology of the limb, trunk, and head, as shown in sagittal sections of thoracic paraspinal skeletal muscle and in coronal sections of the tongue muscle

• in contrast, the morphology of smooth and cardiac muscle appears unaffected

abnormal skeletal muscle fiber morphology ( J:65380 )

• at E14.5, primary skeletal myofibers appear relatively normal but less organized than wild-type myofibers

• at E16.5, and esp. by E18.5, homozygotes exhibit abnormally large, thick, and spherical skeletal myofibers, and lack smaller secondary myofibers normally adjacent to the primary myofibers

• at E16.5 and E18.5, abnormal skeletal myofibers are multinucleated

• at E18.5, but not earlier, nuclei of atypical muscle fibers display poor H&E staining, indicating progressive degeneration of nuclei

nervous system

abnormal neuronal migration ( J:65380 )

• at E18.5, mutant embryos display abnormal positioning of a subset of neurons, as evidenced by localized areas of cellular disorganization in the dentate gyrus of the hippocampus and in the olfactory bulb

• however, overall brain development and gross histology appears normal

abnormal dentate gyrus morphology ( J:65380 )

• at E18.5, homozygotes display localized areas of cellular disorganization in the dentate gyrus

abnormal olfactory bulb morphology ( J:65380 )

• at E16.5 and E18.5, homozygotes display poor organization of the mitral cell layer in the olfactory bulbs

cellular

abnormal neuronal migration ( J:65380 )

• at E18.5, mutant embryos display abnormal positioning of a subset of neurons, as evidenced by localized areas of cellular disorganization in the dentate gyrus of the hippocampus and in the olfactory bulb

• however, overall brain development and gross histology appears normal