Phenotypes associated with this allele

• Allele Symbol: Nos2^tm1Liew
• Allele Name: targeted mutation 1, Foo Y Liew
• Allele ID: MGI:2158787
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nos2^tm1Liew/Nos2^tm1Liew	involves: 129 * 129P2/OlaHsd	MGI:4358423
hm2	Nos2^tm1Liew/Nos2^tm1Liew	involves: 129P2/Ola * MF1	MGI:2672126

Genotype
MGI:4358423

hm1

• Allelic Composition: Nos2^tm1Liew/Nos2^tm1Liew
• Genetic Background: involves: 129 * 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:118561 )

N • macrophage killing of Calmette-Guerin mycobacteria following ATP stimulation is normal

Genotype
MGI:2672126

hm2

• Allelic Composition: Nos2^tm1Liew/Nos2^tm1Liew
• Genetic Background: involves: 129P2/Ola * MF1

immune system

increased T cell proliferation ( J:25755 )

• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice

abnormal T-helper 1 physiology ( J:25755 )

• homozygotes infected with L. major exhibit a significantly stronger Th1 type of immune response than similarly-infected wild-type or heterozygous mice

increased interferon-gamma secretion ( J:25755 )

• in vitro, splenocytes from L. major-infected homozygotes produce more IFN-gamma but less IL-4 in response to soluble leishmania antigens than those from similarly-infected wild-type or heterozygous mice

• in addition, splenocytes from carrageenin-treated homozygotes produce more IFN-gamma than those from similarly-treated wild-type mice when stimulated with ConA

decreased inflammatory response ( J:25755 )

• in the carrageenin-induced paw-edema model, homozygotes develop significantly less footpad swelling than similarly-treated wild-type mice

decreased susceptibility to endotoxin shock ( J:25755 )

• unlike wild-type mice which display up to 12% loss of mass and ~50% mortality by 48 hr after LPS treatment (12.5 mg/kg), all homozygotes are shown to fully recover from an initial transient loss of mass within the first 24 hrs

increased susceptibility to parasitic infection ( J:25755 )

• unlike wild-type and heterozygous mice which are highly resistant to Leishmania major infection and achieve spontaneous healing after footpad injection, homozygotes display higher parasite loads and develop visceral disease by day 70 post-infection, as indicated by a 2- to 3-fold increase in spleen and draining lymph node size

hematopoietic system

increased T cell proliferation ( J:25755 )

• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice

abnormal T-helper 1 physiology ( J:25755 )

• homozygotes infected with L. major exhibit a significantly stronger Th1 type of immune response than similarly-infected wild-type or heterozygous mice

cellular

increased T cell proliferation ( J:25755 )

• in vitro, splenocytes from L. major-infected homozygotes display significantly higher levels of T cell proliferation in response to leishmanial antigens or ConA than those from similarly-infected wild-type or heterozygous mice