Phenotypes associated with this allele

• Allele Symbol: Cd8b1^tm1Wpfl
• Allele Name: targeted mutation 1, Wai-Ping Fung-Leung
• Allele ID: MGI:2158830
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd8b1^tm1Wpfl/Cd8b1^tm1Wpfl	involves: 129S2/SvPas	MGI:2662262

Genotype
MGI:2662262

hm1

• Allelic Composition: Cd8b1^tm1Wpfl/Cd8b1^tm1Wpfl
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:19928 )

N • peripheral CD8⁺ T cells from mutant mice display normal cytotoxic effector function against in vivo viral infection with lymphocytic choriomeningitis virus (LCMV) and vesicular stomatitis virus (VSV)

decreased thymocyte number ( J:19928 )

• the subset of CD8alpha⁺ mature thymocytes is reduced to 20% of that in wild-type control mice

• in contrast, the intraepithelial lymphocyte (IEL) number is within normal range

abnormal T cell subpopulation ratio ( J:19928 )

• in peripheral lymphoid organs, the ratio of CD4⁺ to CD8alpha⁺ T cells is approximately 10:1 versus 1.6:1 in wild-type control mice

abnormal CD8-positive, alpha beta T cell morphology ( J:19928 )

• CD8alpha expression on immature thymocytes and peripheral T cells is reduced to 44% and 53% of wild-type levels, respectively

• in contrast, CD8alpha expression levels on IEL remains unaffected

abnormal CD8-positive, alpha-beta T cell differentiation ( J:19928 )

• homozygotes display reduced CD8alpha expression and thymic maturation of CD8⁺ T cells

decreased CD8-positive, alpha-beta T cell number ( J:19928 )

• in the lymph nodes, the number of CD8alpha⁺ T cells is reduced to 30% of that in wild-type control mice

• the subset of CD8alpha⁺ mature thymocytes is reduced to 20% of that in wild-type control mice

• in contrast, the CD8a⁺ intraepithelial lymphocyte (IEL) population is similar to that in wild-type control mice

hematopoietic system

decreased thymocyte number ( J:19928 )

• the subset of CD8alpha⁺ mature thymocytes is reduced to 20% of that in wild-type control mice

• in contrast, the intraepithelial lymphocyte (IEL) number is within normal range

abnormal T cell subpopulation ratio ( J:19928 )

• in peripheral lymphoid organs, the ratio of CD4⁺ to CD8alpha⁺ T cells is approximately 10:1 versus 1.6:1 in wild-type control mice

abnormal CD8-positive, alpha beta T cell morphology ( J:19928 )

• CD8alpha expression on immature thymocytes and peripheral T cells is reduced to 44% and 53% of wild-type levels, respectively

• in contrast, CD8alpha expression levels on IEL remains unaffected

abnormal CD8-positive, alpha-beta T cell differentiation ( J:19928 )

• homozygotes display reduced CD8alpha expression and thymic maturation of CD8⁺ T cells

decreased CD8-positive, alpha-beta T cell number ( J:19928 )

• in the lymph nodes, the number of CD8alpha⁺ T cells is reduced to 30% of that in wild-type control mice

• the subset of CD8alpha⁺ mature thymocytes is reduced to 20% of that in wild-type control mice

• in contrast, the CD8a⁺ intraepithelial lymphocyte (IEL) population is similar to that in wild-type control mice

endocrine/exocrine glands

decreased thymocyte number ( J:19928 )

• the subset of CD8alpha⁺ mature thymocytes is reduced to 20% of that in wild-type control mice

• in contrast, the intraepithelial lymphocyte (IEL) number is within normal range