Phenotypes associated with this allele

• Allele Symbol: Crem^tm1Saco
• Allele Name: targeted mutation 1, Paolo Sassone-Corsi
• Allele ID: MGI:2158921
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Crem^tm1Saco/Crem^tm1Saco	involves: 129/Sv * C57BL/6	MGI:2181413
hm2	Crem^tm1Saco/Crem^tm1Saco	involves: C57BL/6	MGI:3040661
ht3	Crem^tm1Saco/Crem^+	involves: 129/Sv * C57BL/6	MGI:3040663

Genotype
MGI:2181413

hm1

• Allelic Composition: Crem^tm1Saco/Crem^tm1Saco
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

decreased anxiety-related response ( J:58568 )

• homozygotes show reduced anxiety compared to wild-type littermates

hyperactivity ( J:58568 )

• homozygotes show increased spontaneous activity compared to wild-type littermates

abnormal circadian behavior ( J:58568 )

• homozygotes show homogeneous activity in light and dark period unlike wild-type littermates

cellular

multinucleated giant male germ cells ( J:81370 )

♂ • many multinucleated giant cells are seen in homozygotes

reproductive system

multinucleated giant male germ cells ( J:81370 )

♂ • many multinucleated giant cells are seen in homozygotes

small seminiferous tubules ( J:31890 )

♂ • tubuli from homozygotes are 20 - 30% smaller than those from wild-type mice and lack the normal spermatogenic wave

decreased testis weight ( J:31890 )

♂ • adult testes are 20 - 25% smaller in weight with reduced vascularization in mutants compared to wild-type littermates

arrest of spermatogenesis ( J:31890 )

♂ • only round spermatids are seen in homozygotes

male infertility ( J:31890 )

♂ • males are completely sterile

liver/biliary system

impaired liver regeneration ( J:81370 )

• after partial hepatectomy cell proliferation is significantly reduced in homozygous mutants

endocrine/exocrine glands

small seminiferous tubules ( J:31890 )

♂ • tubuli from homozygotes are 20 - 30% smaller than those from wild-type mice and lack the normal spermatogenic wave

decreased testis weight ( J:31890 )

♂ • adult testes are 20 - 25% smaller in weight with reduced vascularization in mutants compared to wild-type littermates

Genotype
MGI:3040661

hm2

• Allelic Composition: Crem^tm1Saco/Crem^tm1Saco
• Genetic Background: involves: C57BL/6

reproductive system

arrest of spermatogenesis ( J:88151 )

♂ • only round spermatids are seen in homozygotes

male infertility ( J:88151 )

♂ • males are completely sterile

♂ • offspring can be generated using the round spermatids and intracytoplasmic sperm injection

Genotype
MGI:3040663

ht3

• Allelic Composition: Crem^tm1Saco/Crem⁺
• Genetic Background: involves: 129/Sv * C57BL/6

reproductive system

oligozoospermia ( J:31889 )

♂ • seminal fluid from heterozygous males shows a 46% reduction in spermatozoa compared to wild-type males

teratozoospermia ( J:31889 )

♂ • seminal fluid from heterozygous males shows a 35% decrease in the ratio of motile spermatozoa and a 2 fold increase in abnormal spermatozoa

reduced male fertility ( J:31889 )

♂ • average litter size from heterozygous crosses is significantly smaller than heterozygous/homozygous crosses

cellular

oligozoospermia ( J:31889 )

♂ • seminal fluid from heterozygous males shows a 46% reduction in spermatozoa compared to wild-type males

teratozoospermia ( J:31889 )

♂ • seminal fluid from heterozygous males shows a 35% decrease in the ratio of motile spermatozoa and a 2 fold increase in abnormal spermatozoa