Phenotypes associated with this allele

• Allele Symbol: Foxa2^tm1Dnl
• Allele Name: targeted mutation 1, James E Darnell
• Allele ID: MGI:2158924
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Foxa2^tm1Dnl/Foxa2^tm1Dnl	involves: 129P2/OlaHsd * C57BL/6J	MGI:3849567
ht2	Foxa2^tm1Dnl/Foxa2^+	involves: 129P2/OlaHsd * C57BL/6J	MGI:3849568

Genotype
MGI:3849567

hm1

• Allelic Composition: Foxa2^tm1Dnl/Foxa2^tm1Dnl
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:19896 )

• all die between E9.5 and E13

embryo

abnormal endoderm development ( J:19896 )

• at E9.5, endodermal cells are present but have not invaginated to form the gut tube

decreased embryo size ( J:19896 )

• at E9.5

abnormal embryonic tissue morphology ( J:19896 )

• at E7.5 the embryo has a tubular appearance and maintains little contact with the extraembryonic tissues

• at E9.5 embryos are either minute with few externally distinguishable landmarks or small, showing increased elongation compared to minute embryos but decreased compared to control littermates

abnormal chordamesoderm morphology ( J:19896 )

• expression analysis indicates that notochord development is not initiated

abnormal paraxial mesoderm morphology ( J:19896 )

• at E9.5 in many cases the paraxial mesoderm forms a continuous mass of tissue that traverses the midline under the neural tube

abnormal neural tube morphology ( J:19896 )

• the neural tube is closed at E9.5 but the dorsal and ventral regions appear abnormal with a high density of pyknotic nuclei primarily in the dorsal regions and an increase in thickness in the ventral regions

absent floor plate ( J:19896 )

• at E9.5, expression analysis indicates failure of differentiation of floor plate cells at all axial levels

absent notochord ( J:19896 )

• at E8.5 and E9.5

• expression analysis indicates that notochord development is not initiated

absent primitive node ( J:19896 )

• lack a distinct node at E7.5 and E8.5

abnormal somite development ( J:19896 )

• at E9.5 fewer than 10 somites are seen at the trunk level compared to more than 20 in control littermates

• at E9.5 somites are disarranged and in many cases the bilateral separation of segmented mesoderm is lost

cellular

abnormal cell death ( J:19896 )

• at E9.5 embryos contain increased numbers of dying cells

abnormal neuron differentiation ( J:19896 )

• at E9.5, expression analysis indicates absence of motor neuron differentiation in the ventral region of the neural tube

nervous system

abnormal nervous system development ( J:19896 )

• at E8.5 neural tissues are disordered and have prominent ridges

abnormal neuron differentiation ( J:19896 )

• at E9.5, expression analysis indicates absence of motor neuron differentiation in the ventral region of the neural tube

abnormal neural tube morphology ( J:19896 )

• the neural tube is closed at E9.5 but the dorsal and ventral regions appear abnormal with a high density of pyknotic nuclei primarily in the dorsal regions and an increase in thickness in the ventral regions

absent floor plate ( J:19896 )

• at E9.5, expression analysis indicates failure of differentiation of floor plate cells at all axial levels

vision/eye

abnormal eye development ( J:19896 )

• fail to show signs of eye differentiation at E9.5

growth/size/body

decreased embryo size ( J:19896 )

• at E9.5

Genotype
MGI:3849568

ht2

• Allelic Composition: Foxa2^tm1Dnl/Foxa2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

mortality/aging

neonatal lethality, incomplete penetrance ( J:19896 )

• about 10% of mice die within 24 h of birth

growth/size/body

malocclusion ( J:19896 )

• seen in over 50% of mice

reproductive system

absent estrus ( J:19896 )

♀ • almost 50% of females fail to pass through estrus

absent estrous cycle ( J:19896 )

♀

increased miscarriage rate ( J:19896 )

♀ • fewer than 50% of females carry embryos to term

reduced female fertility ( J:19896 )

♀

behavior/neurological

impaired righting response ( J:19896 )

• many are unable to right themselves when placed on their backs

abnormal gait ( J:19896 )

• circular gait

homeostasis/metabolism

increased circulating free fatty acids level ( J:95662 )

increased circulating triglyceride level ( J:95662 )

abnormal glucose homeostasis ( J:95662 )

• hepatic glucose output is elevated and hepatic insulin sensitivity is reduced in mice fed a high fat diet

increased liver triglyceride level ( J:95662 )

• increased hepatic levels

abnormal metabolism ( J:95662 )

• ketogenesis is significantly decreased in mice fed a standard chow diet and this reduction is more severe in mice fed a high fat diet

• beta-oxidation is reduced in mice fed a high fat diet

skeleton

malocclusion ( J:19896 )

• seen in over 50% of mice

craniofacial

malocclusion ( J:19896 )

• seen in over 50% of mice

liver/biliary system

increased liver triglyceride level ( J:95662 )

• increased hepatic levels