Analysis Tools
mortality/aging
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• homozygotes exhibit early embryonic lethality at E5.5 to E6.5
• the timing of lethality appears to reflect the loss and/or dilution of stores of maternal Max, which is expressed at high levels in oocytes
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embryo
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• in vitro, mutant blastocyst outgrowths cease to grow after 2-3 days in culture, exhibitng a reduced inner cell mass (30%-50% reduction in surface area)
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• homozygotes exhibit a generalized developmental arrest soon after implantation (at ~E5.5-E6.5)
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• at E6.5, mutant embryos are under-represented, severely compromised, and 50%-70% smaller than wild-type embryos
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• at E5.5 and E6.5, homozygotes exhibit cellular degeneration, reduced cellularity, and thin germ layers in the absence of significant apoptosis
• at E6.5, homozygotes fail to show distinct embryonic features
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• at E6.5, homozygotes lack a clear demarcation of embryonic and extraembryonic regions
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growth/size/body
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• at E6.5, mutant embryos are under-represented, severely compromised, and 50%-70% smaller than wild-type embryos
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cellular
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• in vitro, mutant blastocyst outgrowths cease to grow after 2-3 days in culture, exhibitng a reduced inner cell mass (30%-50% reduction in surface area)
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