Analysis Tools
cellular
| N |
• in vitro, mutant thymocytes display no significant differences in anti-Fas or dexamethasone-induced apoptosis relative to wild-type thymocytes, as determined by an MTT assay
• in vivo, homozygotes display normal anti-Fas induced apoptosis in liver and kidney cells, as determined by TUNEL staining
• in vitro, mutant mouse embryonic fibroblasts (MEFs) show normal sensitivity to non-endoplasmic reticulum (ER) stress-inducing apoptotic signals (e.g. staurosporine, anti-FAS plus cycloheximide, and TNF plus cycloheximide) relative to wild-type MEFs
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• mutant MEFs are more resistant to endoplasmic reticulum (ER) stress-inducing apoptotic stimuli (e.g. befeldin A, tunicamycin, and thapsigargin) than wild-type MEFs, as determined by a cell viability assay
• at 4 days after i.p. injection of high dose tunicamycin, homozygotes remain viable and exhibit significantly less TUNEL-positive cells and renal tubule epithelial cell damage than similarly-treated wild-type mice, most of which die with extensive hemorrhagic lesions in the renal tubular epithelium
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• in vitro, primary cortical neurons isolated from mutant mice are more resistant to amyloid-beta protein neurotoxicity than wild-type neurons, as determined by the % of neurons with condensed nuclei (8.6% vs 24.9%, respectively)
• in contrast, mutant cortical neurons show no significant protection against neuronal cell death induced by staurosporine or trophic factor deprivation
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homeostasis/metabolism
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• in vitro, primary cortical neurons isolated from mutant mice are more resistant to amyloid-beta protein neurotoxicity than wild-type neurons, as determined by the % of neurons with condensed nuclei (8.6% vs 24.9%, respectively)
• in contrast, mutant cortical neurons show no significant protection against neuronal cell death induced by staurosporine or trophic factor deprivation
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nervous system
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• in vitro, primary cortical neurons isolated from mutant mice are more resistant to amyloid-beta protein neurotoxicity than wild-type neurons, as determined by the % of neurons with condensed nuclei (8.6% vs 24.9%, respectively)
• in contrast, mutant cortical neurons show no significant protection against neuronal cell death induced by staurosporine or trophic factor deprivation
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neoplasm
| N |
• homozygotes are viable, developmentally and behaviorally normal, and do not display an increased incidence of tumors relative to wild-type mice
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