Phenotypes associated with this allele

• Allele Symbol: Tg(Prm-cre)58Og
• Allele Name: transgene insertion 58, Stephen O'Gorman
• Allele ID: MGI:2176182
• Summary: 11 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pth2^tm1Vlcg/Pth2^tm1Vlcg Tg(CAG-LacZ,-Pth2)1Tbu/0 Tg(Prm-cre)58Og/0	involves: 129 * C57BL/6	MGI:3808008
cn2	Ogt^tm1Gwh/Ogt^+ Tg(Prm-cre)58Og/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:3841629
cn3	Cask^tm1Sud/Cask^tm1.1Sud Tg(Prm-cre)58Og/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ	MGI:3699502
cn4	Meig1^tm1.1Zzha/Meig1^tm1.1Zzha Tg(Prm-cre)58Og/0	involves: 129S1/SvImJ * 129S4/SvJae	MGI:6852583
cn5	Fyco1^tm1.1Arte/Fyco1^tm1.1Arte Tg(Prm-cre)58Og/0	involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6J * C57BL/6NTac	MGI:7531471
cn6	Kras^tm4Tyj/Kras^+ Tg(Prm-cre)58Og/0	involves: 129S4/SvJae	MGI:3716392
cn7	Mettl3^tm1.1Jhha/Mettl3^tm1.1Jhha Tg(Prm-cre)58Og/0	involves: 129S4/SvJae * 129S4/SvJaeSor * BALB/c * C57BL/6	MGI:6491904
cn8	Huwe1^tm1Alas/Y Tg(Prm-cre)58Og/0	involves: 129S4/SvJae * 129S7/SvEvBrd	MGI:7286185
cn9	Inpp5b^tm2Nbm/Inpp5b^tm2Nbm Tg(Prm-cre)58Og/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:3040923
cn10	Fyco1^tm1.1Arte/Fyco1^tm1.1Arte Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/0 Tg(Prm-cre)58Og/0	involves: 129S/Sv * C57BL/6J * C57BL/6N * C57BL/6NTac	MGI:7531479
cn11	Snx20^tm1Lex/Snx20^tm1Lex Tg(Prm-cre)58Og/0	involves: 129S/SvEvBrd * 129S4/SvJae	MGI:3774652

Genotype
MGI:3808008

cn1

• Allelic Composition: Pth2^tm1Vlcg/Pth2^tm1Vlcg; Tg(CAG-LacZ,-Pth2)1Tbu/0; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:138142 )

N • male fertility is restored

female infertility ( J:138142 )

♀ • persists

Genotype
MGI:3841629

cn2

• Allelic Composition: Ogt^tm1Gwh/Ogt⁺; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

normal phenotype

no abnormal phenotype detected ( J:93112 )

♀ • mice are viable and fertile with no gross abnormalities and expression levels of Ogt that are similar to controls suggesting inactivation of the paternal X chromosome in all tissues and cell types surveyed

♀ • mice do not produce any offspring carrying the recombined allele

Genotype
MGI:3699502

cn3

• Allelic Composition: Cask^tm1Sud/Cask^tm1.1Sud; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ

Cleft palate in Cask^tm1Sud/Cask^tm1.1Sud Tg(Prm-cre)580g/0 mice

mortality/aging

neonatal lethality, complete penetrance ( J:117978 )

♀ • die within a few hours of birth

craniofacial

palatal shelves fail to meet at midline ( J:117978 )

♀

cleft secondary palate ( J:117978 )

♀ • observed in homozygotes with ~80% penetrance

digestive/alimentary system

palatal shelves fail to meet at midline ( J:117978 )

♀

cleft secondary palate ( J:117978 )

♀ • observed in homozygotes with ~80% penetrance

nervous system

nervous system phenotype ( J:117978 )

♀N • synapse formation and membrane properties of cultured neurons are not significantly different from wild-type neurons; vesicle pool sizes and evoked neurotransmitter release are similar in mutant and wild-type neurons

increased neuron apoptosis ( J:117978 )

♀ • number of apoptotic neurons in thalamus is increased 3-fold compared to controls

abnormal neurotransmitter secretion ( J:117978 )

♀ • excitatory spontaneous mini events show 2-fold increase in frequency in cultured neurons, while inhibitory minifrequency is decreased ~1.4 fold

respiratory system

respiratory failure ( J:117978 )

♀ • plethysmography of newborns shows severe postnatal respiratory failure

cellular

increased neuron apoptosis ( J:117978 )

♀ • number of apoptotic neurons in thalamus is increased 3-fold compared to controls

growth/size/body

palatal shelves fail to meet at midline ( J:117978 )

♀

cleft secondary palate ( J:117978 )

♀ • observed in homozygotes with ~80% penetrance

Genotype
MGI:6852583

cn4

• Allelic Composition: Meig1^tm1.1Zzha/Meig1^tm1.1Zzha; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S1/SvImJ * 129S4/SvJae

reproductive system

reproductive system phenotype ( J:194944 )

♂N • all males are fertile and sire normal numbers of offspring

♂N • testis weight to body weight and seminal vesicle weight to body weight ratios are normal

♂N • epididymidal sperm count, testis as well as epididymal histology and sperm morphology are normal, and no reduction in sperm motility is observed

Genotype
MGI:7531471

cn5

• Allelic Composition: Fyco1^tm1.1Arte/Fyco1^tm1.1Arte; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S1/SvImJ * 129S4/SvJae * C57BL/6J * C57BL/6NTac

vision/eye

abnormal lens epithelium morphology ( J:336796 )

• at P60, lens epithelial cells show extensive vacuolization

abnormal lens fiber morphology ( J:336796 )

• at P60, lens fiber cells are large and display irregular shapes

• at P0, P7, and P14, differentiating lens fiber cells show abnormal retention of cellular organelles, including endoplasmic reticulum (ER), mitochondria, and Golgi apparatus (GA), suggesting impaired organelle removal

cataract ( J:336796 )

• mice show initial signs of lens opacities at 4 weeks that develop into mild cataracts at ~8 weeks of age

mature cataract ( J:336796 )

• mice develop mature bilateral cataracts by 16 weeks of age; extent of lens opacities is variable among mice

cellular

abnormal Golgi apparatus morphology ( J:336796 )

• at P14, lenses show an increased Golgi apparatus mass relative to age-matched wild-type lenses

abnormal endoplasmic reticulum morphology ( J:336796 )

• at P0, P7, P14 and P21, lenses show an increased ER mass relative to age-matched wild-type lenses

impaired autophagy ( J:336796 )

• at P0, lenses show alterations in many autophagy-associated genes, proteins and lipids, including a 1.48-fold increase in the level of SQSTM1/p62 and a modest accumulation of phosphatidylethanolamine (PE) phospholipids, suggesting impaired autophagy

homeostasis/metabolism

impaired autophagy ( J:336796 )

• at P0, lenses show alterations in many autophagy-associated genes, proteins and lipids, including a 1.48-fold increase in the level of SQSTM1/p62 and a modest accumulation of phosphatidylethanolamine (PE) phospholipids, suggesting impaired autophagy

abnormal phospholipid level ( J:336796 )

• at P0, lenses show a modest accumulation of phosphatidylethanolamine (PE) phospholipids

Genotype
MGI:3716392

cn6

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S4/SvJae

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:119477 )

• mutants die between E9.5 and 11.5

embryo

absent vitelline blood vessels ( J:119477 )

• at E9.5 vasculature is poorly developed; vasculature has a primitive honeycomb-like network lacking branching vitelline vessels, while large vitelline vessels are absent

increased embryonic tissue cell apoptosis ( J:119477 )

• at death, embryos exhibit widespread apoptosis

embryonic growth arrest ( J:119477 )

• embryos show developmental arrest

abnormal placenta labyrinth morphology ( J:119477 )

• marked defect in inner labyrinth layer is observed at E9.5

abnormal placental labyrinth vasculature morphology ( J:119477 )

• fetal blood vessels underlying inner labyrinth layer are absent

pale yolk sac ( J:119477 )

• at E9.5, yolk sacs are pale and roughened

cellular

increased embryonic tissue cell apoptosis ( J:119477 )

• at death, embryos exhibit widespread apoptosis

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:119477 )

• fetal blood vessels underlying inner labyrinth layer are absent

absent vitelline blood vessels ( J:119477 )

• at E9.5 vasculature is poorly developed; vasculature has a primitive honeycomb-like network lacking branching vitelline vessels, while large vitelline vessels are absent

Genotype
MGI:6491904

cn7

• Allelic Composition: Mettl3^tm1.1Jhha/Mettl3^tm1.1Jhha; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S4/SvJae * 129S4/SvJaeSor * BALB/c * C57BL/6

reproductive system

reproductive system phenotype ( J:299108 )

♂N • normal male fertility and seminiferous tubules morphology

Genotype
MGI:7286185

cn8

• Allelic Composition: Huwe1^tm1Alas/Y; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S4/SvJae * 129S7/SvEvBrd

reproductive system

reproductive system phenotype ( J:323709 )

♂N • male mice are fertile and show normal spermatogenesis with no significant differences in sperm number, sperm motility or testis weights relative to control males

Genotype
MGI:3040923

cn9

• Allelic Composition: Inpp5b^tm2Nbm/Inpp5b^tm2Nbm; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

reproductive system

reproductive system phenotype ( J:73678 )

N • mutant males display normal fertility with normal litter sizes

Genotype
MGI:7531479

cn10

• Allelic Composition: Fyco1^tm1.1Arte/Fyco1^tm1.1Arte; Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/0; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S/Sv * C57BL/6J * C57BL/6N * C57BL/6NTac

cellular

impaired autophagy ( J:336796 )

• multiphoton laser scanning microscopy showed a 1.3- and 1.6-fold higher GFP intensity in the whole lens and the anterior lens including lens epithelium, respectively, indicating a reduced autophagic flux in mouse lenses

homeostasis/metabolism

impaired autophagy ( J:336796 )

• multiphoton laser scanning microscopy showed a 1.3- and 1.6-fold higher GFP intensity in the whole lens and the anterior lens including lens epithelium, respectively, indicating a reduced autophagic flux in mouse lenses

Genotype
MGI:3774652

cn11

• Allelic Composition: Snx20^tm1Lex/Snx20^tm1Lex; Tg(Prm-cre)58Og/0
• Genetic Background: involves: 129S/SvEvBrd * 129S4/SvJae

normal phenotype

no abnormal phenotype detected ( J:131282 )

• mice are overall normal and healthy

immune system

immune system phenotype ( J:131282 )

N • mice exhibit normal neutrophil morphology and physiology