Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-cre)548Jxm
• Allele Name: transgene insertion 548, Jamey Marth
• Allele ID: MGI:2176199
• Summary: 53 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(NOTCH1/GFP)Xhsu/Gt(ROSA)26Sor^+ Tg(Lck-cre)548Jxm/0	B6.Cg-Gt(ROSA)26Sor^tm1(NOTCH1/GFP)Xhsu Tg(Lck-cre)548Jxm	MGI:5432021
cn2	Hnrnpl^tm1.1Tmo/Hnrnpl^tm1.1Tmo Tg(Lck-cre)548Jxm/0	B6.Cg-Hnrnpl^tm1.1Tmo Tg(Lck-cre)548Jxm	MGI:5442376
cn3	Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm/0	B6.Cg-Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm	MGI:5447539
cn4	Nr3c1^tm1.1Jda/Nr3c1^+ Tg(Lck-cre)548Jxm/0	B6.Cg-Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm	MGI:5447540
cn5	Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda Rag2^tm1.1Cgn/Rag2^tm1.1Cgn Tg(Lck-cre)548Jxm/0 Tg(TcrAND)53Hed/0	B6.Cg-Rag2^tm1.1Cgn Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm Tg(TcrAND)53Hed	MGI:5447537
cn6	Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda Tcra^tm1Mom/Tcra^tm1Mom Tg(Lck-cre)548Jxm/0	B6.Cg-Tcra^tm1Mom Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm	MGI:5447538
cn7	Zbtb17^tm1Cksn/Zbtb17^tm1Cksn Tg(Lck-cre)548Jxm/0	B6.Cg-Zbtb17^tm1Cksn Tg(Lck-cre)548Jxm	MGI:5006711
cn8	Arhgef12^tm1.1Wet/Arhgef12^tm1.1Wet Tg(Lck-cre)548Jxm/0	involves: 129 * C57BL/6 * CBA	MGI:3849204
cn9	Crebbp^tm1Jvd/Crebbp^tm1Jvd Tg(Lck-cre)548Jxm/0	involves: 129 * C57BL/6 * CBA	MGI:3618585
cn10	Cops5^tm1Rpar/Cops5^tm1Rpar Tg(Lck-cre)548Jxm/0 Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129 * C57BL/6 * CBA * SJL	MGI:3783542
cn11	Brca2^tm1Mak/Brca2^tm2Mak Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3831545
cn12	Casp8^tm1Raz/Casp8^tm1Raz Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:2655731
cn13	Ep300^tm2Pkb/Ep300^tm2Pkb Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3618539
cn14	Crebbp^tm1Jvd/Crebbp^tm1Jvd Ep300^tm2Pkb/Ep300^tm2Pkb Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3618581
cn15	Grb2^tm1Paw/Grb2^+ Pten^tm2Mak/Pten^tm2Mak Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3717275
cn16	Tg(Lck-cre)548Jxm/? Upf2^tm1Btp/Upf2^tm1Btp	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3797492
cn17	Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3831544
cn18	Brca1^tm2Mak/Brca1^tm2Mak Tg(Lck-cre)548Jxm/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834729
cn19	Brca1^tm2Mak/Brca1^tm2Mak Tg(Lck-cre)548Jxm/? Trp53^tm1Brd/Trp53^tm1Brd	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834730
cn20	Brca1^tm2Mak/Brca1^tm2Mak Tg(BCL2)36Wehi/? Tg(Lck-cre)548Jxm/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834731
cn21	Brca1^tm2Mak/Brca1^tm2Mak Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj Tg(Lck-cre)548Jxm/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834732
cn22	Brca1^tm2Mak/Brca1^tm2Mak Tg(Lck-cre)548Jxm/? Tg(TcrLCMV)327Sdz/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834733
cn23	Brca1^tm2Mak/Brca1^tm2Mak Chek2^tm1Mak/Chek2^tm1Mak Tg(Lck-cre)548Jxm/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3834734
cn24	Myb^tm1Cgn/Myb^tm1Cgn Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:3047332
cn25	Myb^tm1Cgn/Myb^tm1.1Cgn Tg(Lck-cre)548Jxm/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2	MGI:3047331
cn26	Gt(ROSA)26Sor^tm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor^+ Hnrnpl^tm1.1Tmo/Hnrnpl^tm1.1Tmo Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:5442377
cn27	Myb^tm1Epr/Myb^tm1Epr Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:3511178
cn28	Ogt^tm1Gwh/Y Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:3841625
cn29	Bak1^tm1Thsn/Bak1^tm1Thsn Bax^tm2Sjk/Bax^tm2Sjk Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:5562713
cn30	Cops5^tm1Rpar/Cops5^tm1Rpar Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL	MGI:3783540
cn31	Cops5^tm1Rpar/Cops5^tm1Rpar Tg(Lck-cre)548Jxm/0 Tg(TcraTcrb)425Cbn/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL	MGI:3783541
cn32	Gna12^tm1Citb/Gna12^tm1Citb Gna13^tm2.1Soff/Gna13^tm2.1Soff Tg(Lck-cre)548Jxm/0	involves: 129S1/Sv * C57BL/6 * CBA	MGI:3849203
cn33	Tgfb1^tm1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/?	involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * BALB/c * C57BL/6 * CBA	MGI:3849995
cn34	Mcl1^tm1Ywh/Mcl1^tm1Ywh Tg(Lck-cre)548Jxm/0	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * CBA	MGI:3801464
cn35	Trpm7^tm1Clph/Trpm7^tm1.1Clph Tg(Lck-cre)548Jxm/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3814863
cn36	Cflar^tm1Ywh/Cflar^tm1Ywh Tg(Lck-cre)548Jxm/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * CBA	MGI:3590081
cn37	Bcl2l1^tm1Ywh/Bcl2l1^tm1Ywh Tg(Lck-cre)548Jxm/?	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * CBA	MGI:3582307
cn38	Gt(ROSA)26Sor^tm2Sho/? Tg(Lck-cre)548Jxm/0	involves: 129S4/SvJaeSor * C57BL/6 * SJL	MGI:3696481
cn39	Tg(Lck-cre)548Jxm/? Tgfb1^tm2.1Doe/Tgfb1^tm2.1Doe	involves: 129S6/SvEvTac * Black Swiss * C57BL/6 * CBA	MGI:3849994
cn40	Nr3c1^tm2Ljm/Nr3c1^tm2Ljm Tg(Lck-cre)548Jxm/?	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:3716820
cn41	Cr1l^tm1.1Song/Cr1l^tm1.1Song Tg(Lck-cre)548Jxm/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * SJL	MGI:3838225
cn42	Map3k7^tm1Zjc/Map3k7^tm1Zjc Tg(Lck-cre)548Jxm/0	involves: 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3701507
cn43	Atg3^tm1.1Ywh/Atg3^tm1.1Ywh Tg(Lck-cre)548Jxm/0	involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA	MGI:5009306
cn44	Snai3^tm1.1Jhws/Snai3^tm1.1Jhws Tg(Lck-cre)548Jxm/0	involves: 129/Sv * C57BL/6 * CBA	MGI:5619846
cn45	Gt(ROSA)26Sor^tm1(EYFP)Cos/? Tg(Lck-cre)548Jxm/0	involves: 129X1/SvJ * C57BL/6 * SJL	MGI:3696478
cn46	Ppp4c^tm1Tht/Ppp4c^tm1Tht Tg(Lck-cre)548Jxm/0 Tg(TcraTcrb)425Cbn/0	involves: BALB/c * C57BL/6 * CBA	MGI:3699364
cn47	Lat2^tm2Wz/Lat2^tm2Wz Tg(Lck-cre)548Jxm/0	involves: C57BL/6	MGI:3695390
cn48	Ppp4c^tm1Tht/Ppp4c^tm1Tht Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * CBA	MGI:3699363
cn49	Fas^tm1Ach/Fas^tm1Ach Tg(Lck-cre)548Jxm/?	involves: C57BL/6 * CBA	MGI:3720606
cn50	Septin9^tm2.1Emfu/Septin9^tm2.1Emfu Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * CBA	MGI:5544263
cn51	Map3k3^tm1Bisu/Map3k3^tm2Bisu Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * CBA	MGI:3836810
cn52	Bcap31^tm1.1Bwang/Bcap31^tm1.1Bwang Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * CBA	MGI:6719029
cn53	Gt(ROSA)26Sor^tm1Hjf/? Tg(Lck-cre)548Jxm/0	involves: C57BL/6 * SJL	MGI:3696482

Genotype
MGI:5432021

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(NOTCH1/GFP)Xhsu}/Gt(ROSA)26Sor⁺; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Gt(ROSA)26Sor^{tm1(NOTCH1/GFP)Xhsu} Tg(Lck-cre)548Jxm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:185287 )

• median survival of 10 weeks

neoplasm

increased T cell derived lymphoma incidence ( J:185287 )

• large tumors with metastasis to lymph nodes, livers and/or kidney

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:185287 )

• large tumors with metastasis to lymph nodes, livers and/or kidney

immune system

increased T cell derived lymphoma incidence ( J:185287 )

• large tumors with metastasis to lymph nodes, livers and/or kidney

hematopoietic system

increased T cell derived lymphoma incidence ( J:185287 )

• large tumors with metastasis to lymph nodes, livers and/or kidney

Genotype
MGI:5442376

cn2

• Allelic Composition: Hnrnpl^tm1.1Tmo/Hnrnpl^tm1.1Tmo; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Hnrnpl^tm1.1Tmo Tg(Lck-cre)548Jxm

immune system

increased T cell proliferation ( J:188749 )

• in DN4 and CD4SP cells following the TCR beta selection checkpoint

thymus hypoplasia ( J:188749 )

• 75% reduction, cell-autonomous

abnormal T cell differentiation ( J:188749 )

• thymocyte chemotaxis is impaired

increased double-negative T cell number ( J:188749 )

• slight

decreased double-positive T cell number ( J:188749 )

decreased T cell number ( J:188749 )

• reduction is specific to TCR alpha-beta cells

• in the periphery

• however, the number of gamma-delta cells is normal

decreased CD4-positive, alpha-beta T cell number ( J:188749 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:188749 )

• in the thymus

hematopoietic system

increased T cell proliferation ( J:188749 )

• in DN4 and CD4SP cells following the TCR beta selection checkpoint

thymus hypoplasia ( J:188749 )

• 75% reduction, cell-autonomous

abnormal T cell differentiation ( J:188749 )

• thymocyte chemotaxis is impaired

increased double-negative T cell number ( J:188749 )

• slight

decreased T cell number ( J:188749 )

• reduction is specific to TCR alpha-beta cells

• in the periphery

• however, the number of gamma-delta cells is normal

decreased double-positive T cell number ( J:188749 )

decreased CD4-positive, alpha-beta T cell number ( J:188749 )

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:188749 )

• in the thymus

endocrine/exocrine glands

thymus hypoplasia ( J:188749 )

• 75% reduction, cell-autonomous

cellular

increased T cell proliferation ( J:188749 )

• in DN4 and CD4SP cells following the TCR beta selection checkpoint

Genotype
MGI:5447539

cn3

• Allelic Composition: Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm

immune system

decreased thymocyte apoptosis ( J:189951 )

• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells

thymus hypoplasia ( J:189951 )

decreased double-positive T cell number ( J:189951 )

• in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:189951 )

• modestly in the periphery

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:189951 )

• modestly in the periphery

• in the thymus

decreased single-positive T cell number ( J:189951 )

• of mature single positive cells in the thymus

abnormal humoral immune response ( J:189951 )

• polyclonal T cells exhibit attenuated response to an antigen compared with wild-type cells

cellular

decreased thymocyte apoptosis ( J:189951 )

• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells

hematopoietic system

decreased thymocyte apoptosis ( J:189951 )

• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells

thymus hypoplasia ( J:189951 )

decreased double-positive T cell number ( J:189951 )

• in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:189951 )

• modestly in the periphery

• in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:189951 )

• modestly in the periphery

• in the thymus

decreased single-positive T cell number ( J:189951 )

• of mature single positive cells in the thymus

endocrine/exocrine glands

decreased thymocyte apoptosis ( J:189951 )

• double positive thymocytes are resistant to glucocorticoid-induced apoptosis compared with wild-type cells

thymus hypoplasia ( J:189951 )

Genotype
MGI:5447540

cn4

• Allelic Composition: Nr3c1^tm1.1Jda/Nr3c1⁺; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm

immune system

decreased thymocyte apoptosis ( J:189951 )

• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells

cellular

decreased thymocyte apoptosis ( J:189951 )

• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells

hematopoietic system

decreased thymocyte apoptosis ( J:189951 )

• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells

endocrine/exocrine glands

decreased thymocyte apoptosis ( J:189951 )

• thymocytes are less sensitive to dexamethasone-induced apoptosis compared with wild-type cells

Genotype
MGI:5447537

cn5

• Allelic Composition: Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda; Rag2^tm1.1Cgn/Rag2^tm1.1Cgn; Tg(Lck-cre)548Jxm/0; Tg(TcrAND)53Hed/0
• Genetic Background: B6.Cg-Rag2^tm1.1Cgn Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm Tg(TcrAND)53Hed

immune system

decreased double-positive T cell number ( J:189951 )

• in the thymus

hematopoietic system

decreased double-positive T cell number ( J:189951 )

• in the thymus

Genotype
MGI:5447538

cn6

• Allelic Composition: Nr3c1^tm1.1Jda/Nr3c1^tm1.1Jda; Tcra^tm1Mom/Tcra^tm1Mom; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Tcra^tm1Mom Nr3c1^tm1.1Jda Tg(Lck-cre)548Jxm

immune system

decreased double-positive T cell number ( J:189951 )

• in the thymus

hematopoietic system

decreased double-positive T cell number ( J:189951 )

• in the thymus

Genotype
MGI:5006711

cn7

• Allelic Composition: Zbtb17^tm1Cksn/Zbtb17^tm1Cksn; Tg(Lck-cre)548Jxm/0
• Genetic Background: B6.Cg-Zbtb17^tm1Cksn Tg(Lck-cre)548Jxm

immune system

immune system phenotype ( J:170508 )

N • mice exhibit normal thymic cellularity and development

Genotype
MGI:3849204

cn8

• Allelic Composition: Arhgef12^tm1.1Wet/Arhgef12^tm1.1Wet; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129 * C57BL/6 * CBA

hematopoietic system

abnormal T cell proliferation ( J:149560 )

• CD4⁺ T cells have less impairment of in vitro proliferation when a RhoA activator is used

immune system

abnormal T cell proliferation ( J:149560 )

• CD4⁺ T cells have less impairment of in vitro proliferation when a RhoA activator is used

cellular

abnormal T cell proliferation ( J:149560 )

• CD4⁺ T cells have less impairment of in vitro proliferation when a RhoA activator is used

Genotype
MGI:3618585

cn9

• Allelic Composition: Crebbp^tm1Jvd/Crebbp^tm1Jvd; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129 * C57BL/6 * CBA

immune system

small thymus ( J:105505 )

increased T cell derived lymphoma incidence ( J:105505 )

• appear as early as 16 weeks of age

increased double-positive T cell number ( J:105505 )

• increased 5 fold

decreased T cell number ( J:105505 )

• reduced about 2 fold in peripheral blood

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

decreased CD4-positive, alpha-beta T cell number ( J:105505 )

• reduced in numbers but normal numbers of CD8+ cells

neoplasm

increased T cell derived lymphoma incidence ( J:105505 )

• appear as early as 16 weeks of age

hematopoietic system

small thymus ( J:105505 )

increased T cell derived lymphoma incidence ( J:105505 )

• appear as early as 16 weeks of age

increased double-positive T cell number ( J:105505 )

• increased 5 fold

decreased T cell number ( J:105505 )

• reduced about 2 fold in peripheral blood

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

decreased CD4-positive, alpha-beta T cell number ( J:105505 )

• reduced in numbers but normal numbers of CD8+ cells

endocrine/exocrine glands

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

increased T cell derived lymphoma incidence ( J:105505 )

• appear as early as 16 weeks of age

Genotype
MGI:3783542

cn10

• Allelic Composition: Cops5^tm1Rpar/Cops5^tm1Rpar; Tg(Lck-cre)548Jxm/0; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129 * C57BL/6 * CBA * SJL

hematopoietic system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency

immune system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency

endocrine/exocrine glands

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control showing no rescue of the reduced thymic cellularity resulting from Cops5 deficiency

Genotype
MGI:3831545

cn11

• Allelic Composition: Brca2^tm1Mak/Brca2^tm2Mak; Trp53^tm1Brd/Trp53^tm1Brd; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * CBA

immune system

immune system phenotype ( J:79697 )

N • proliferating T cells fail to exhibit the same increase in spontaneous apoptosis observed in Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm mice

increased activated T cell number ( J:79697 )

• likely due to decreased apoptosis

neoplasm

increased tumor incidence ( J:79697 )

• tumorigenesis is accelerated compared to in wild-type and Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm mice but not compared to in Trp53^tm1Brd homozygotes

increased lymphoma incidence ( J:79697 )

• 90% of mice succumb to T-cell lymphomas compared to 70% of Trp53^tm1Brd/Trp53^tm1Brd Tg(Lck-cre)548Jxm mice

increased malignant tumor incidence ( J:79697 )

• mice develop high grade malignancies

increased osteosarcoma incidence ( J:79697 )

cellular

abnormal chromosome morphology ( J:79697 )

• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in Brca2^tm1Mak/Brca2^tm2Mak Tg(Lck-cre)548Jxm mice or wild-type cells

decreased cellular sensitivity to ionizing radiation ( J:79697 )

• thymocytes are resistant to ionizing radiation-induced cell death unlike wild-type cells

• however, response to UV- or methyl methane sulfonate-induced cell death is normal

hematopoietic system

increased activated T cell number ( J:79697 )

• likely due to decreased apoptosis

skeleton

increased osteosarcoma incidence ( J:79697 )

Genotype
MGI:2655731

cn12

• Allelic Composition: Casp8^tm1Raz/Casp8^tm1Raz; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

mortality/aging

premature death ( J:107455 )

• die at an average age of 51.7 weeks, however lethality is sometimes seen as early as 14 weeks of age

growth/size/body

decreased body size ( J:107455 )

decreased body weight ( J:107455 )

enlarged spleen ( J:107455 )

immune system

enlarged spleen ( J:107455 )

decreased leukocyte cell number ( J:107455 )

• peripheral T cell lymphopenia is seen early in life but is no longer apparent in older mutants

abnormal lymphocyte morphology ( J:107455 )

• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased B-to-T cell ratio

increased lymphocyte cell number ( J:107455 )

• lymphoproliferation in older mutants is confirmed by increased total lymphocyte counts

increased B cell number ( J:107455 )

• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery

abnormal T cell morphology ( J:82759 )

abnormal T cell subpopulation ratio ( J:82759 , J:107455 )

• the CD4+ to CD8+ T-cell ratio is increased in spleen, lymph node, and peripheral blood (J:82759)

• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased proportion of CD8+ T cells relative to CD4+ T cells (J:107455)

decreased T cell number ( J:82759 )

• relative proportion of splenic and lymph node T cells is significantly lower in young mutants

decreased CD4-positive, alpha-beta T cell number ( J:82759 )

• lower numbers of CD4+ T cells in young mutants

decreased CD8-positive, alpha-beta T cell number ( J:82759 )

• lower numbers of CD8+ T cells in young mutants

increased T cell number ( J:107455 )

• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery

abnormal T cell physiology ( J:107455 )

• older mutants accumulate nonclonal T cell infiltrates in the lungs, liver, and kidneys accompanied by tissue damage

• T cell infiltration is seen as early as 20 weeks of age and progresses as mice age

decreased T cell apoptosis ( J:82759 )

• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal

abnormal CD8-positive, alpha-beta T cell physiology ( J:82759 )

• do not exhibit an expansion of CD8+ T cells in the peripheral blood 6 days after lymphocytic choriomeningitis virus (LCMV) infection and show decreased representation of CD62L^highCD44^low (memory) T cells

• LCMV-infected mutants are unable to generate CD8+ cytotoxic T cells specific for LCMV

abnormal T cell activation ( J:107455 )

• isolated T cells from old mutants are in a perpetual state of activation

abnormal T cell proliferation ( J:107455 )

• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis

decreased T cell proliferation ( J:82759 )

• exhibit defective activation-induced expansion of peripheral T cells

enlarged lymph nodes ( J:107455 )

• in older mutants

lymphoid hyperplasia ( J:107455 )

• lymphoid hyperplasia is apparent in older mutants

hematopoietic system

enlarged spleen ( J:107455 )

decreased leukocyte cell number ( J:107455 )

• peripheral T cell lymphopenia is seen early in life but is no longer apparent in older mutants

abnormal lymphocyte morphology ( J:107455 )

• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased B-to-T cell ratio

increased lymphocyte cell number ( J:107455 )

• lymphoproliferation in older mutants is confirmed by increased total lymphocyte counts

increased B cell number ( J:107455 )

• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery

abnormal T cell morphology ( J:82759 )

abnormal T cell subpopulation ratio ( J:82759 , J:107455 )

• the CD4+ to CD8+ T-cell ratio is increased in spleen, lymph node, and peripheral blood (J:82759)

• peripheral lymphocyte populations from spleens and lymph nodes of old mutants show a decreased proportion of CD8+ T cells relative to CD4+ T cells (J:107455)

decreased T cell number ( J:82759 )

• relative proportion of splenic and lymph node T cells is significantly lower in young mutants

decreased CD4-positive, alpha-beta T cell number ( J:82759 )

• lower numbers of CD4+ T cells in young mutants

decreased CD8-positive, alpha-beta T cell number ( J:82759 )

• lower numbers of CD8+ T cells in young mutants

increased T cell number ( J:107455 )

• older mutants exhibit a balanced expansion of both B and T lymphocyte numbers in the periphery

abnormal T cell physiology ( J:107455 )

• older mutants accumulate nonclonal T cell infiltrates in the lungs, liver, and kidneys accompanied by tissue damage

• T cell infiltration is seen as early as 20 weeks of age and progresses as mice age

decreased T cell apoptosis ( J:82759 )

• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal

abnormal CD8-positive, alpha-beta T cell physiology ( J:82759 )

• do not exhibit an expansion of CD8+ T cells in the peripheral blood 6 days after lymphocytic choriomeningitis virus (LCMV) infection and show decreased representation of CD62L^highCD44^low (memory) T cells

• LCMV-infected mutants are unable to generate CD8+ cytotoxic T cells specific for LCMV

abnormal T cell activation ( J:107455 )

• isolated T cells from old mutants are in a perpetual state of activation

abnormal T cell proliferation ( J:107455 )

• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis

decreased T cell proliferation ( J:82759 )

• exhibit defective activation-induced expansion of peripheral T cells

liver/biliary system

abnormal liver morphology ( J:107455 )

• livers from 30-week old mutants display T cell accumulation as focal perivascular infiltrates

renal/urinary system

abnormal kidney morphology ( J:107455 )

• kidneys from 30-week old mutants show focal interstitial and periglomerular T cell infiltration

respiratory system

abnormal lung morphology ( J:107455 )

• lungs from 30-week old mutants contain obvious interstitial peribronchiovascular T cell infiltration

• at about 1 year of age, disrupted lung tissue organization is associated with much more widespread and abundant T cell infiltration

cellular

decreased T cell apoptosis ( J:82759 )

• thymocytes and activated T cells are resistant to CD95L-induced apoptosis, however mitochondria-mediated apoptosis is normal

abnormal T cell proliferation ( J:107455 )

• T cells derived from older mutants exhibit defective in vitro proliferative responses to various stimuli and activated T cells are resistant to CD95L-induced apoptosis

decreased T cell proliferation ( J:82759 )

• exhibit defective activation-induced expansion of peripheral T cells

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autoimmune lymphoproliferative syndrome type 2B		DOID:0110116	OMIM:607271	J:107455

Genotype
MGI:3618539

cn13

• Allelic Composition: Ep300^tm2Pkb/Ep300^tm2Pkb; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

increased double-positive T cell number ( J:105505 )

• increased 3 fold

decreased single-positive T cell number ( J:105505 )

• half the normal numbers of single positive thymocyte

• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood

hematopoietic system

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

increased double-positive T cell number ( J:105505 )

• increased 3 fold

decreased single-positive T cell number ( J:105505 )

• half the normal numbers of single positive thymocyte

• proportion of CD4+ to CD8+ is normal in spleen and peripheral blood

endocrine/exocrine glands

small thymus ( J:105505 )

decreased thymocyte number ( J:105505 )

• total number of thymocytes significantly reduced

Genotype
MGI:3618581

cn14

• Allelic Composition: Crebbp^tm1Jvd/Crebbp^tm1Jvd; Ep300^tm2Pkb/Ep300^tm2Pkb; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

abnormal T cell number ( J:105505 )

• drop in number of single positive thymocytes comparable to the increase in double negative cells

• no increase in CD8+ single positive thymocytes

• near absence of single positive thymocytes in the periphery

increased double-negative T cell number ( J:105505 )

• 10 fold increase in the percentage of double negative thymocytes

decreased double-positive T cell number ( J:105505 )

• near absence in the periphery

hematopoietic system

abnormal T cell number ( J:105505 )

• drop in number of single positive thymocytes comparable to the increase in double negative cells

• no increase in CD8+ single positive thymocytes

• near absence of single positive thymocytes in the periphery

increased double-negative T cell number ( J:105505 )

• 10 fold increase in the percentage of double negative thymocytes

decreased double-positive T cell number ( J:105505 )

• near absence in the periphery

Genotype
MGI:3717275

cn15

• Allelic Composition: Grb2^tm1Paw/Grb2⁺; Pten^tm2Mak/Pten^tm2Mak; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

neoplasm

increased T cell derived lymphoma incidence ( J:93530 )

• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:93530 )

• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes

immune system

increased T cell derived lymphoma incidence ( J:93530 )

• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes

hematopoietic system

increased T cell derived lymphoma incidence ( J:93530 )

• thymic lymphomas develop with a mean age of onset of 60 days, similar to that seen in T cell-specific Pten homozygotes

Genotype
MGI:3797492

cn16

• Allelic Composition: Tg(Lck-cre)548Jxm/?; Upf2^tm1Btp/Upf2^tm1Btp
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

thymus hypoplasia ( J:134765 )

• significant reduction in size of the thymus with cellularity decreased almost 3-fold

• the percentage of apoptotic cells in the thymus is doubled

abnormal T cell receptor beta chain V(D)J recombination ( J:134765 )

• unproductive transcripts with premature stop codons is detectable in 29% of single positive T cells

decreased double-positive T cell number ( J:134765 )

• there is a 3-fold reduction in the number of double positive T cells found in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:134765 )

• there is a 4- to 5-fold reduction in CD4 T cells found in the thymus

• the number of CD4 T cells found in the periphery is 30% that of wild-type

decreased CD8-positive, alpha-beta T cell number ( J:134765 )

• there is a 2-fold to 3-fold reduction in T cell numbers

• the number of CD8 T cells found in the periphery is 30% that of wild-type

hematopoietic system

thymus hypoplasia ( J:134765 )

• significant reduction in size of the thymus with cellularity decreased almost 3-fold

• the percentage of apoptotic cells in the thymus is doubled

abnormal T cell receptor beta chain V(D)J recombination ( J:134765 )

• unproductive transcripts with premature stop codons is detectable in 29% of single positive T cells

decreased double-positive T cell number ( J:134765 )

• there is a 3-fold reduction in the number of double positive T cells found in the thymus

decreased CD4-positive, alpha-beta T cell number ( J:134765 )

• there is a 4- to 5-fold reduction in CD4 T cells found in the thymus

• the number of CD4 T cells found in the periphery is 30% that of wild-type

decreased CD8-positive, alpha-beta T cell number ( J:134765 )

• there is a 2-fold to 3-fold reduction in T cell numbers

• the number of CD8 T cells found in the periphery is 30% that of wild-type

endocrine/exocrine glands

thymus hypoplasia ( J:134765 )

• significant reduction in size of the thymus with cellularity decreased almost 3-fold

• the percentage of apoptotic cells in the thymus is doubled

Genotype
MGI:3831544

cn17

• Allelic Composition: Brca2^tm1Mak/Brca2^tm2Mak; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

immune system phenotype ( J:79697 )

N • T cell development and proliferation are normal

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal

neoplasm

neoplasm ( J:79697 )

N • mice do not develop tumors

cellular

cellular phenotype ( J:79697 )

N • response to DNA damaging agents is normal

abnormal chromosome morphology ( J:79697 )

• T cells exhibit increased frequencies of chromatid or chromosomal breaks, tri-radial structures, and chromosomal fragments compared to in wild-type cells

aneuploidy ( J:79697 )

• 14.5% of T cells exhibit aneuploidy unlike wild-type cells

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal

hematopoietic system

increased T cell apoptosis ( J:79697 )

• proliferating T cells exhibit increased spontaneous apoptosis compared to wild-type cells

• however, apoptosis of activated T cells following exposure to ionizing radiation, adriamycin or FasL is normal

Genotype
MGI:3834729

cn18

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

decreased T cell proliferation ( J:63282 )

• reduced proliferation when stimulated with monoclonal antibody to CD3e

abnormal T cell number ( J:63282 )

increased double-negative T cell number ( J:63282 )

• increased proportion of double negative thymocytes

• absolute number of double negative thymocytes is normal

decreased double-positive T cell number ( J:63282 )

• less than 10% of number in controls

decreased single-positive T cell number ( J:63282 )

• less than 10% of number in controls

abnormal immune system organ morphology ( J:63282 )

decreased thymocyte number ( J:63282 )

• 90% reduction in thymocyte number

cellular

abnormal cell cycle checkpoint function ( J:63282 )

• fewer T cells entering S phase

abnormal cell death ( J:63282 )

• reduced viability of thymocytes in culture

• reduced survival of T cells when stimulated with monoclonal antibody to CD3e

increased cellular sensitivity to gamma-irradiation ( J:63282 )

• increased thymocyte sensitivity of gamma-irradiation

decreased T cell proliferation ( J:63282 )

• reduced proliferation when stimulated with monoclonal antibody to CD3e

neoplasm

increased tumor incidence ( J:90512 )

• only about 12% with tumors

• most tumors are peripheral rather than thymic

hematopoietic system

decreased T cell proliferation ( J:63282 )

• reduced proliferation when stimulated with monoclonal antibody to CD3e

abnormal T cell number ( J:63282 )

decreased thymocyte number ( J:63282 )

• 90% reduction in thymocyte number

increased double-negative T cell number ( J:63282 )

• increased proportion of double negative thymocytes

• absolute number of double negative thymocytes is normal

decreased double-positive T cell number ( J:63282 )

• less than 10% of number in controls

decreased single-positive T cell number ( J:63282 )

• less than 10% of number in controls

endocrine/exocrine glands

decreased thymocyte number ( J:63282 )

• 90% reduction in thymocyte number

Genotype
MGI:3834730

cn19

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Tg(Lck-cre)548Jxm/?; Trp53^tm1Brd/Trp53^tm1Brd
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:90512 )

• increased incidence

• reduced latency

mortality/aging

premature death ( J:90512 )

• most mice with thymic lymphomas are moribund or die by 3 months of age

cellular

chromosomal instability ( J:63282 )

• increased frequency of aberrations such as polyploidy, quadriradial chromosomes, and telomeric translocations

spontaneous chromosome breakage ( J:63282 )

immune system

immune system phenotype ( J:63282 )

N • thymus cellularity restored

N • T cell numbers in lymph nodes are improved

increased T cell derived lymphoma incidence ( J:90512 )

• increased incidence

• reduced latency

neoplasm

increased T cell derived lymphoma incidence ( J:90512 )

• increased incidence

• reduced latency

hematopoietic system

increased T cell derived lymphoma incidence ( J:90512 )

• increased incidence

• reduced latency

Genotype
MGI:3834731

cn20

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Tg(BCL2)36Wehi/?; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

cellular

chromosomal instability ( J:63282 )

• increased frequency of aberrations such as polyploidy, quadriradial chromosomes, and telomeric translocations

spontaneous chromosome breakage ( J:63282 )

immune system

immune system phenotype ( J:63282 )

N • thymus cellularity restored

Genotype
MGI:3834732

cn21

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

immune system phenotype ( J:63282 )

N • T cell numbers in lymph nodes are more nearly normal

decreased thymocyte number ( J:63282 )

hematopoietic system

decreased thymocyte number ( J:63282 )

endocrine/exocrine glands

decreased thymocyte number ( J:63282 )

Genotype
MGI:3834733

cn22

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Tg(Lck-cre)548Jxm/?; Tg(TcrLCMV)327Sdz/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

immune system

decreased T cell proliferation ( J:63282 )

abnormal T cell number ( J:63282 )

decreased thymocyte number ( J:63282 )

increased double-negative T cell number ( J:63282 )

decreased double-positive T cell number ( J:63282 )

hematopoietic system

decreased T cell proliferation ( J:63282 )

abnormal T cell number ( J:63282 )

decreased thymocyte number ( J:63282 )

increased double-negative T cell number ( J:63282 )

decreased double-positive T cell number ( J:63282 )

endocrine/exocrine glands

decreased thymocyte number ( J:63282 )

cellular

decreased T cell proliferation ( J:63282 )

Genotype
MGI:3834734

cn23

• Allelic Composition: Brca1^tm2Mak/Brca1^tm2Mak; Chek2^tm1Mak/Chek2^tm1Mak; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:90512 )

• 32% with lymphomas by 180 days of age

• tumors are restricted to the thymus

immune system

immune system phenotype ( J:90512 )

N • complete thymocyte restoration

N • T cell numbers in the periphery are restored

increased T cell derived lymphoma incidence ( J:90512 )

• 32% with lymphomas by 180 days of age

• tumors are restricted to the thymus

cellular

cellular phenotype ( J:90512 )

N • resistant to gamma irradiation

N • restoration of normal cell cycle arrest of T cells when stimulated with antibody to CD3e or CD3 and Il2

chromosomal instability ( J:90512 )

• increased frequency

neoplasm

increased T cell derived lymphoma incidence ( J:90512 )

• 32% with lymphomas by 180 days of age

• tumors are restricted to the thymus

hematopoietic system

increased T cell derived lymphoma incidence ( J:90512 )

• 32% with lymphomas by 180 days of age

• tumors are restricted to the thymus

Genotype
MGI:3047332

cn24

• Allelic Composition: Myb^tm1Cgn/Myb^tm1Cgn; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 75%

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is decreased about 80%

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is decreased about 80%

immune system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 75%

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is decreased about 80%

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is decreased about 80%

endocrine/exocrine glands

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced about 75%

Genotype
MGI:3047331

cn25

• Allelic Composition: Myb^tm1Cgn/Myb^tm1.1Cgn; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

hematopoietic system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is decreased

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is decreased

immune system

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced

decreased double-positive T cell number ( J:91122 )

• the absolute number of double positive cells is decreased

decreased CD4-positive, alpha-beta T cell number ( J:91122 )

• the absolute number of CD4 single positive cells is decreased

endocrine/exocrine glands

thymus hypoplasia ( J:91122 )

• thymus cellularity is reduced

Genotype
MGI:5442377

cn26

• Allelic Composition: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺; Hnrnpl^tm1.1Tmo/Hnrnpl^tm1.1Tmo; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

immune system

abnormal T cell differentiation ( J:188749 )

• T cells either do not leave thymus or fail to migrate into the blood and to the peripheral lymphoid organs

hematopoietic system

abnormal T cell differentiation ( J:188749 )

• T cells either do not leave thymus or fail to migrate into the blood and to the peripheral lymphoid organs

Genotype
MGI:3511178

cn27

• Allelic Composition: Myb^tm1Epr/Myb^tm1Epr; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

hematopoietic system

thymus hypoplasia ( J:93481 )

• cellularity of the thymus is reduced to 11% of controls

abnormal T cell differentiation ( J:93481 )

abnormal double-negative T cell morphology ( J:93481 )

• thymocytes accumulate at the DN3 stage

• reduced numbers of cells at the DN4 stage

decreased double-positive T cell number ( J:93481 )

• double positive cell population reduced to 62% of normal

immune system

thymus hypoplasia ( J:93481 )

• cellularity of the thymus is reduced to 11% of controls

abnormal T cell differentiation ( J:93481 )

abnormal double-negative T cell morphology ( J:93481 )

• thymocytes accumulate at the DN3 stage

• reduced numbers of cells at the DN4 stage

decreased double-positive T cell number ( J:93481 )

• double positive cell population reduced to 62% of normal

endocrine/exocrine glands

thymus hypoplasia ( J:93481 )

• cellularity of the thymus is reduced to 11% of controls

Genotype
MGI:3841625

cn28

• Allelic Composition: Ogt^tm1Gwh/Y; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

immune system

increased T cell apoptosis ( J:93112 )

♂ • increase in annexin-V expression in single positive T cells

decreased T cell number ( J:93112 )

♂ • about a 75% reduction in the number of T cells in the lymph nodes and spleen

♂ • no T cells carrying the recombined allele are found in the lymph nodes or spleen

decreased thymocyte number ( J:93112 )

♂ • about a 50% reduction in thymocyte cell number

decreased double-positive T cell number ( J:93112 )

♂ • cortical double positive T cells are reduced by 50%

decreased CD4-positive, alpha-beta T cell number ( J:93112 )

♂ • reduced by 50%

decreased CD8-positive, alpha-beta T cell number ( J:93112 )

♂ • reduced by 75%

hematopoietic system

increased T cell apoptosis ( J:93112 )

♂ • increase in annexin-V expression in single positive T cells

decreased T cell number ( J:93112 )

♂ • about a 75% reduction in the number of T cells in the lymph nodes and spleen

♂ • no T cells carrying the recombined allele are found in the lymph nodes or spleen

decreased thymocyte number ( J:93112 )

♂ • about a 50% reduction in thymocyte cell number

decreased double-positive T cell number ( J:93112 )

♂ • cortical double positive T cells are reduced by 50%

decreased CD4-positive, alpha-beta T cell number ( J:93112 )

♂ • reduced by 50%

decreased CD8-positive, alpha-beta T cell number ( J:93112 )

♂ • reduced by 75%

cellular

increased T cell apoptosis ( J:93112 )

♂ • increase in annexin-V expression in single positive T cells

endocrine/exocrine glands

decreased thymocyte number ( J:93112 )

♂ • about a 50% reduction in thymocyte cell number

Genotype
MGI:5562713

cn29

• Allelic Composition: Bak1^tm1Thsn/Bak1^tm1Thsn; Bax^tm2Sjk/Bax^tm2Sjk; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

immune system

increased regulatory T cell number ( J:208318 )

• at 15 months as in Bcl2l11^tm1.1Ast homozygotes

hematopoietic system

increased regulatory T cell number ( J:208318 )

• at 15 months as in Bcl2l11^tm1.1Ast homozygotes

Genotype
MGI:3783540

cn30

• Allelic Composition: Cops5^tm1Rpar/Cops5^tm1Rpar; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL

hematopoietic system

increased T cell apoptosis ( J:132114 )

• in DN and DP thymocytes

thymus medulla hypoplasia ( J:132114 )

• a severely hypoplastic medulla

decreased thymocyte number ( J:132114 )

• an 80-90% reduction

decreased double-positive T cell number ( J:132114 )

• a marked decrease in the proportion of DP and SP thymocytes

• DN thymocytes percentage and absolute numbers were not significantly altered

decreased single-positive T cell number ( J:132114 )

• a marked decrease in the proportion of DP and SP thymocytes

immune system

increased T cell apoptosis ( J:132114 )

• in DN and DP thymocytes

thymus medulla hypoplasia ( J:132114 )

• a severely hypoplastic medulla

decreased thymocyte number ( J:132114 )

• an 80-90% reduction

decreased double-positive T cell number ( J:132114 )

• a marked decrease in the proportion of DP and SP thymocytes

• DN thymocytes percentage and absolute numbers were not significantly altered

decreased single-positive T cell number ( J:132114 )

• a marked decrease in the proportion of DP and SP thymocytes

cellular

abnormal cell cycle checkpoint function ( J:132114 )

• cell cycle arrest at S/G2/M phase in DN thymocyte

increased T cell apoptosis ( J:132114 )

• in DN and DP thymocytes

endocrine/exocrine glands

thymus medulla hypoplasia ( J:132114 )

• a severely hypoplastic medulla

decreased thymocyte number ( J:132114 )

• an 80-90% reduction

Genotype
MGI:3783541

cn31

• Allelic Composition: Cops5^tm1Rpar/Cops5^tm1Rpar; Tg(Lck-cre)548Jxm/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL

hematopoietic system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

immune system

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

endocrine/exocrine glands

decreased thymocyte number ( J:132114 )

• an 80-90% reduction compared to control

Genotype
MGI:3849203

cn32

• Allelic Composition: Gna12^tm1Citb/Gna12^tm1Citb; Gna13^tm2.1Soff/Gna13^tm2.1Soff; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S1/Sv * C57BL/6 * CBA

immune system

increased T cell proliferation ( J:149560 )

• homeostatic CD4⁺ T cell proliferation is increased within the lymph nodes

• allogenic stimulation of CD4⁺ T cells is also increased

thymus hyperplasia ( J:149560 )

• thymus weight and cellularity is increased by about a third

increased CD4-positive, alpha-beta T cell number ( J:149560 )

• CD4⁺ T cell numbers in the lymph nodes and blood are increased by about a third

increased CD8-positive, alpha-beta T cell number ( J:149560 )

• CD8⁺ T cell numbers in the lymph nodes are slightly increased

abnormal CD4-positive, alpha-beta T cell physiology ( J:149560 )

• CD4⁺ T cells have longer interactions between themselves and with allogenic dendritic cells

• CD4⁺ T cells have a higher affinity for ICAM-coated surfaces

• CD4⁺ T cells have decreased motility through transwell filters

• CD4⁺ T cells transferred into wild-type mice have enhanced enhanced transendothelial migration into lymph nodes

lymph node hyperplasia ( J:149560 )

• axillary, cervical, inguinal and mesenteric lymph nodes are increased in weight and cell number by about a third

increased susceptibility to type IV hypersensitivity reaction ( J:149560 )

• an increase in ear thickness is observed after sensitization and challenge with DNFB

• CD4⁺ T cells in cervical lymph nodes have a higher proliferation rate than those of control mice after DNFB challenge

increased interleukin-2 secretion ( J:149560 )

• IL-2 secretion is enhanced by CD4⁺ T cells in response to allogenic stimulation

increased susceptibility to autoimmune diabetes ( J:149560 )

• diabetes development is accelerated and aggravated after streptozotocin induction with high glucose levels detected

• peripancreatic lymph nodes are enlarged 5-6 days after disease induction

• increased numbers of CD4⁺ T cells are noted in the pancreas 14 days after disease induction compared to controls

hematopoietic system

increased T cell proliferation ( J:149560 )

• homeostatic CD4⁺ T cell proliferation is increased within the lymph nodes

• allogenic stimulation of CD4⁺ T cells is also increased

thymus hyperplasia ( J:149560 )

• thymus weight and cellularity is increased by about a third

increased CD4-positive, alpha-beta T cell number ( J:149560 )

• CD4⁺ T cell numbers in the lymph nodes and blood are increased by about a third

increased CD8-positive, alpha-beta T cell number ( J:149560 )

• CD8⁺ T cell numbers in the lymph nodes are slightly increased

abnormal CD4-positive, alpha-beta T cell physiology ( J:149560 )

• CD4⁺ T cells have longer interactions between themselves and with allogenic dendritic cells

• CD4⁺ T cells have a higher affinity for ICAM-coated surfaces

• CD4⁺ T cells have decreased motility through transwell filters

• CD4⁺ T cells transferred into wild-type mice have enhanced enhanced transendothelial migration into lymph nodes

endocrine/exocrine glands

thymus hyperplasia ( J:149560 )

• thymus weight and cellularity is increased by about a third

cellular

increased T cell proliferation ( J:149560 )

• homeostatic CD4⁺ T cell proliferation is increased within the lymph nodes

• allogenic stimulation of CD4⁺ T cells is also increased

Genotype
MGI:3849995

cn33

• Allelic Composition: Tgfb1^tm1Doe/Tgfb1^tm2.1Doe; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss * BALB/c * C57BL/6 * CBA

Lung and salivary gland inflammation in Tgfb1^tm2.1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (b,e) and Tgfb1^tm1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (c,f) mice

immune system

salivary gland inflammation ( J:150024 )

• salivary gland inflammation is observed in mice at 5 months of age

lung inflammation ( J:150024 )

• inflammation is observed in the lungs at 5 months of age

respiratory system

lung inflammation ( J:150024 )

• inflammation is observed in the lungs at 5 months of age

digestive/alimentary system

salivary gland inflammation ( J:150024 )

• salivary gland inflammation is observed in mice at 5 months of age

endocrine/exocrine glands

salivary gland inflammation ( J:150024 )

• salivary gland inflammation is observed in mice at 5 months of age

Genotype
MGI:3801464

cn34

• Allelic Composition: Mcl1^tm1Ywh/Mcl1^tm1Ywh; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * CBA

immune system

increased T cell apoptosis ( J:137400 )

• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice

thymus hypoplasia ( J:137400 )

• thymus contain 5% of the cells found in wild-type mice

abnormal T cell differentiation ( J:137400 )

• T cell development is blocked between DN2/3 and DN4 stages

• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice

decreased double-negative T cell number ( J:137400 )

• 5% to 10% of wild-type

decreased double-positive T cell number ( J:137400 )

• 40% of wild-type

decreased T cell number ( J:137400 )

• T cell numbers in the spleen are 40% to 50% of wild-type

decreased CD4-positive, alpha-beta T cell number ( J:137400 )

• 5% to 10% of wild-type

decreased CD8-positive, alpha-beta T cell number ( J:137400 )

• 5% to 10% of wild-type

hematopoietic system

increased T cell apoptosis ( J:137400 )

• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice

thymus hypoplasia ( J:137400 )

• thymus contain 5% of the cells found in wild-type mice

abnormal T cell differentiation ( J:137400 )

• T cell development is blocked between DN2/3 and DN4 stages

• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice

decreased T cell number ( J:137400 )

• T cell numbers in the spleen are 40% to 50% of wild-type

decreased double-negative T cell number ( J:137400 )

• 5% to 10% of wild-type

decreased double-positive T cell number ( J:137400 )

• 40% of wild-type

decreased CD4-positive, alpha-beta T cell number ( J:137400 )

• 5% to 10% of wild-type

decreased CD8-positive, alpha-beta T cell number ( J:137400 )

• 5% to 10% of wild-type

cellular

increased T cell apoptosis ( J:137400 )

• apoptosis of DN thymocytes is increased 2-fold compared to in wild-type mice

endocrine/exocrine glands

thymus hypoplasia ( J:137400 )

• thymus contain 5% of the cells found in wild-type mice

Genotype
MGI:3814863

cn35

• Allelic Composition: Trpm7^tm1Clph/Trpm7^tm1.1Clph; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

immune system

abnormal thymus morphology ( J:140630 )

• 85% penetrance of thymic abnormalities

• CD3+ T cells are found distributed throughout the thymus

abnormal thymus cell ratio ( J:140630 )

• increase in the percentage and number of double negative T cells

• increase in the percentage of cells in the DN3 stage

abnormal thymus corticomedullary boundary morphology ( J:140630 )

• the boundary between the cortical and medullary regions is not well defined

abnormal thymus medulla morphology ( J:140630 )

• progressive loss of thymic medullary cells

decreased thymocyte number ( J:140630 )

• substantial reduction in the number of thymocytes

abnormal T cell differentiation ( J:140630 )

• partial block in the transition from double negative to double positive T cells

• block appears to be at the DN3 to DN4 transition as a significant proportion of cells fail to down regulate CD25

decreased T cell number ( J:140630 )

• slight decrease in the percentage and number of T cells in the spleen and lymph nodes but not in the intestine

abnormal T cell physiology ( J:140630 )

• thymocytes lack Mg2+ inhibitable current

• however, Mg2+ influx into freshly isolated T cells is unaffected

hematopoietic system

abnormal thymus morphology ( J:140630 )

• 85% penetrance of thymic abnormalities

• CD3+ T cells are found distributed throughout the thymus

abnormal thymus cell ratio ( J:140630 )

• increase in the percentage and number of double negative T cells

• increase in the percentage of cells in the DN3 stage

abnormal thymus corticomedullary boundary morphology ( J:140630 )

• the boundary between the cortical and medullary regions is not well defined

abnormal thymus medulla morphology ( J:140630 )

• progressive loss of thymic medullary cells

decreased thymocyte number ( J:140630 )

• substantial reduction in the number of thymocytes

abnormal T cell differentiation ( J:140630 )

• partial block in the transition from double negative to double positive T cells

• block appears to be at the DN3 to DN4 transition as a significant proportion of cells fail to down regulate CD25

decreased T cell number ( J:140630 )

• slight decrease in the percentage and number of T cells in the spleen and lymph nodes but not in the intestine

abnormal T cell physiology ( J:140630 )

• thymocytes lack Mg2+ inhibitable current

• however, Mg2+ influx into freshly isolated T cells is unaffected

endocrine/exocrine glands

abnormal thymus morphology ( J:140630 )

• 85% penetrance of thymic abnormalities

• CD3+ T cells are found distributed throughout the thymus

abnormal thymus cell ratio ( J:140630 )

• increase in the percentage and number of double negative T cells

• increase in the percentage of cells in the DN3 stage

abnormal thymus corticomedullary boundary morphology ( J:140630 )

• the boundary between the cortical and medullary regions is not well defined

abnormal thymus medulla morphology ( J:140630 )

• progressive loss of thymic medullary cells

decreased thymocyte number ( J:140630 )

• substantial reduction in the number of thymocytes

Genotype
MGI:3590081

cn36

• Allelic Composition: Cflar^tm1Ywh/Cflar^tm1Ywh; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * CBA

hematopoietic system

increased thymocyte apoptosis ( J:100558 )

• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation

• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)

decreased single-positive T cell number ( J:100558 )

• total thymocyte numbers were not significantly different from wild-type

• in 3-5 weeks old mice the percentages of CD4+ and CD8+ single-positive thymocytes were reduced by 50-70%

• the numbers of CD4+ and CD8+ T lymphocytes in peripheral blood, spleen and lymph nodes were significantly reduced

immune system

increased thymocyte apoptosis ( J:100558 )

• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation

• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)

decreased single-positive T cell number ( J:100558 )

• total thymocyte numbers were not significantly different from wild-type

• in 3-5 weeks old mice the percentages of CD4+ and CD8+ single-positive thymocytes were reduced by 50-70%

• the numbers of CD4+ and CD8+ T lymphocytes in peripheral blood, spleen and lymph nodes were significantly reduced

cellular

increased thymocyte apoptosis ( J:100558 )

• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation

• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)

endocrine/exocrine glands

increased thymocyte apoptosis ( J:100558 )

• thymocytes from mutant mice shows enhanced apoptosis to TCR/CD3 and Fas stimulation

• without stimulation, three- to fourfold increases of apoptotic cells in freshly isolated CD4+ and CD8+ single-positive thymocytes over the control cells (there was no such increase for mutant double-positive cells)

Genotype
MGI:3582307

cn37

• Allelic Composition: Bcl2l1^tm1Ywh/Bcl2l1^tm1Ywh; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * CBA

immune system

decreased thymocyte number ( J:99012 )

• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%

• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%

• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development

decreased double-positive T cell number ( J:99012 )

• after 1-2 days of culture, the number of cells in the double-positive compartment were dramatically reduced

hematopoietic system

decreased thymocyte number ( J:99012 )

• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%

• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%

• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development

decreased double-positive T cell number ( J:99012 )

• after 1-2 days of culture, the number of cells in the double-positive compartment were dramatically reduced

endocrine/exocrine glands

decreased thymocyte number ( J:99012 )

• homozygous mice that are also hemizygous or homozygous mice for Tg(Lck-cre)548Jxm, in which cre recombinase is active in T cell lineage, display: total thymic cellularity was reduced by 40-50%

• the number of mature CD4 positive and CD8 positive T lymphocytes in the spleen was reduced by 40-50%

• however, mutant has a normal CD4- positive and CD8-positive effector and memory T cell development

Genotype
MGI:3696481

cn38

• Allelic Composition: Gt(ROSA)26Sor^tm2Sho/?; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected ( J:117041 )

• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells

Genotype
MGI:3849994

cn39

• Allelic Composition: Tg(Lck-cre)548Jxm/?; Tgfb1^tm2.1Doe/Tgfb1^tm2.1Doe
• Genetic Background: involves: 129S6/SvEvTac * Black Swiss * C57BL/6 * CBA

Lung and salivary gland inflammation in Tgfb1^tm2.1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (b,e) and Tgfb1^tm1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (c,f) mice

growth/size/body

weight loss ( J:150024 )

• there is more than a 10% reduction in mean weight of mice compared to controls at 5 months of age

digestive/alimentary system

salivary gland inflammation ( J:150024 )

• modest salivary gland inflammation is observed in mice at 5 months of age

immune system

abnormal T cell subpopulation ratio ( J:150024 )

• there are slightly more CD8⁺ T cells than CD4⁺ T cells among splenocytes

abnormal T cell activation ( J:150024 )

• there is a higher percentage of T cells expressing activation markers

abnormal immune system organ morphology ( J:150024 )

decreased thymus weight ( J:150024 )

• thymus weight at 5 months of age is reduced by more than a third

thymus hypoplasia ( J:150024 )

• thymus cellularity is reduced by more than two-thirds at 1 month of age

decreased spleen weight ( J:150024 )

• spleen weight is reduced by about a third at 2 months of age

spleen hypoplasia ( J:150024 )

• spleen cellularity is about half that of controls at 5 months of age

increased interferon-gamma secretion ( J:150024 )

• interferon-gamma secretion is enhanced in mutant T cells when activated with various stimuli

increased interleukin-2 secretion ( J:150024 )

• interleukin-2 secretion is enhanced in mutant T cells when activated with various stimuli

increased inflammatory response ( J:150024 )

salivary gland inflammation ( J:150024 )

• modest salivary gland inflammation is observed in mice at 5 months of age

lung inflammation ( J:150024 )

• modest inflammation is observed in the lungs at 5 months of age

respiratory system

lung inflammation ( J:150024 )

• modest inflammation is observed in the lungs at 5 months of age

endocrine/exocrine glands

salivary gland inflammation ( J:150024 )

• modest salivary gland inflammation is observed in mice at 5 months of age

decreased thymus weight ( J:150024 )

• thymus weight at 5 months of age is reduced by more than a third

thymus hypoplasia ( J:150024 )

• thymus cellularity is reduced by more than two-thirds at 1 month of age

hematopoietic system

decreased thymus weight ( J:150024 )

• thymus weight at 5 months of age is reduced by more than a third

thymus hypoplasia ( J:150024 )

• thymus cellularity is reduced by more than two-thirds at 1 month of age

abnormal T cell subpopulation ratio ( J:150024 )

• there are slightly more CD8⁺ T cells than CD4⁺ T cells among splenocytes

decreased spleen weight ( J:150024 )

• spleen weight is reduced by about a third at 2 months of age

spleen hypoplasia ( J:150024 )

• spleen cellularity is about half that of controls at 5 months of age

abnormal T cell activation ( J:150024 )

• there is a higher percentage of T cells expressing activation markers

Genotype
MGI:3716820

cn40

• Allelic Composition: Nr3c1^tm2Ljm/Nr3c1^tm2Ljm; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:97484 )

• mice have increased mortality following treatment with a CD3epsilon antibody

• mortality increases following treatment with staphylococcal enterotoxin A (SEA)

• however, treatment with CD3epsilon antibody or staphylococcal enterotoxin A (SEA) and a COX-2 inhibitor reverses the increase in mortality

immune system

cecum inflammation ( J:97484 )

• treatment with a CD3epsilon antibody leads to inflammation of the cecum

abnormal interferon level ( J:97484 )

• 2 hours after CD3epsilon antibody treatment, interferon-gamma levels are increased

abnormal interleukin level ( J:97484 )

• 8 hours after CD3epsilon antibody treatment, IL-6 levels are increased

abnormal tumor necrosis factor level ( J:97484 )

• 2 hours after CD3epsilon antibody treatment, TNF-alpha levels are increased

increased susceptibility to endotoxin shock ( J:97484 )

• mortality increases following treatment with staphylococcal enterotoxin A (SEA)

digestive/alimentary system

abnormal cecum morphology ( J:97484 )

• treatment with a CD3epsilon antibody leads to increased gastrointestinal damage in the cecum including mucosal and submucosal edema and inflammation along with necrotic regions that are denuded of mucosa

cecum inflammation ( J:97484 )

• treatment with a CD3epsilon antibody leads to inflammation of the cecum

homeostasis/metabolism

abnormal interferon level ( J:97484 )

• 2 hours after CD3epsilon antibody treatment, interferon-gamma levels are increased

abnormal interleukin level ( J:97484 )

• 8 hours after CD3epsilon antibody treatment, IL-6 levels are increased

abnormal tumor necrosis factor level ( J:97484 )

• 2 hours after CD3epsilon antibody treatment, TNF-alpha levels are increased

Genotype
MGI:3838225

cn41

• Allelic Composition: Cr1l^tm1.1Song/Cr1l^tm1.1Song; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * SJL

immune system

increased B cell number ( J:146538 )

• in the peripheral blood

decreased lymphocyte cell number ( J:146538 )

• mice exhibit T cell lymphopenia

• however, chemical depletion of macrophages ameliorates T cell lymphopenia

decreased CD4-positive, alpha-beta T cell number ( J:146538 )

• CD4+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice

• however, thymic CD4+ counts are normal

decreased CD8-positive, alpha-beta T cell number ( J:146538 )

• CD8+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice

• however, thymic CD8+ counts are normal

lymph node hypoplasia ( J:146538 )

hematopoietic system

increased B cell number ( J:146538 )

• in the peripheral blood

decreased lymphocyte cell number ( J:146538 )

• mice exhibit T cell lymphopenia

• however, chemical depletion of macrophages ameliorates T cell lymphopenia

decreased CD4-positive, alpha-beta T cell number ( J:146538 )

• CD4+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice

• however, thymic CD4+ counts are normal

decreased CD8-positive, alpha-beta T cell number ( J:146538 )

• CD8+ T cells are reduced in the spleens, lymph nodes, and peripheral blood mononuclear cells compared to in wild-type mice

• however, thymic CD8+ counts are normal

Genotype
MGI:3701507

cn42

• Allelic Composition: Map3k7^tm1Zjc/Map3k7^tm1Zjc; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * CBA

hematopoietic system

increased thymocyte apoptosis ( J:111794 )

• mutant single positive thymocytes are more sensitive to apoptosis than wild-type

decreased T cell number ( J:111794 )

• percentage of T cells in peripheral lymphoid organs is significantly lower than in controls

decreased CD4-positive, alpha-beta T cell number ( J:111794 )

• mature CD4+ T cells are reduced in number compared to controls

decreased CD8-positive, alpha-beta T cell number ( J:111794 )

• mature CD8+ T cells are reduced in number compared to controls

immune system

increased thymocyte apoptosis ( J:111794 )

• mutant single positive thymocytes are more sensitive to apoptosis than wild-type

decreased T cell number ( J:111794 )

• percentage of T cells in peripheral lymphoid organs is significantly lower than in controls

decreased CD4-positive, alpha-beta T cell number ( J:111794 )

• mature CD4+ T cells are reduced in number compared to controls

decreased CD8-positive, alpha-beta T cell number ( J:111794 )

• mature CD8+ T cells are reduced in number compared to controls

cellular

increased thymocyte apoptosis ( J:111794 )

• mutant single positive thymocytes are more sensitive to apoptosis than wild-type

endocrine/exocrine glands

increased thymocyte apoptosis ( J:111794 )

• mutant single positive thymocytes are more sensitive to apoptosis than wild-type

Genotype
MGI:5009306

cn43

• Allelic Composition: Atg3^tm1.1Ywh/Atg3^tm1.1Ywh; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * CBA

homeostasis/metabolism

impaired autophagy ( J:172749 )

• T cells stimulated with anti-CD3 antibodies exhibit impaired autophagy compared with wild-type cells

immune system

increased T cell apoptosis ( J:172749 )

• in the spleen

decreased T cell proliferation ( J:172749 )

• of T cells stimulated with anti-CD3 antibodies

thymus hypoplasia ( J:172749 )

abnormal T cell morphology ( J:172749 )

• T lymphocytes exhibit increased mitochondria and endoplasmic reticulum content compared with wild-type cells

decreased CD4-positive, alpha-beta T cell number ( J:172749 )

• in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:172749 )

• in the spleen

spleen hypoplasia ( J:172749 )

cellular

impaired autophagy ( J:172749 )

• T cells stimulated with anti-CD3 antibodies exhibit impaired autophagy compared with wild-type cells

increased T cell apoptosis ( J:172749 )

• in the spleen

decreased T cell proliferation ( J:172749 )

• of T cells stimulated with anti-CD3 antibodies

oxidative stress ( J:172749 )

• T cells exhibit overproduction of reactive oxygen species compared with wild-type cells

hematopoietic system

increased T cell apoptosis ( J:172749 )

• in the spleen

decreased T cell proliferation ( J:172749 )

• of T cells stimulated with anti-CD3 antibodies

thymus hypoplasia ( J:172749 )

abnormal T cell morphology ( J:172749 )

• T lymphocytes exhibit increased mitochondria and endoplasmic reticulum content compared with wild-type cells

decreased CD4-positive, alpha-beta T cell number ( J:172749 )

• in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:172749 )

• in the spleen

spleen hypoplasia ( J:172749 )

endocrine/exocrine glands

thymus hypoplasia ( J:172749 )

Genotype
MGI:5619846

cn44

• Allelic Composition: Snai3^tm1.1Jhws/Snai3^tm1.1Jhws; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129/Sv * C57BL/6 * CBA

immune system

immune system phenotype ( J:204710 )

N • T cell populations in thymus, spleen and blood are similar to controls as assessed by FACS analysis

Genotype
MGI:3696478

cn45

• Allelic Composition: Gt(ROSA)26Sor^tm1(EYFP)Cos/?; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected ( J:117041 )

• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells

Genotype
MGI:3699364

cn46

• Allelic Composition: Ppp4c^tm1Tht/Ppp4c^tm1Tht; Tg(Lck-cre)548Jxm/0; Tg(TcraTcrb)425Cbn/0
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

hematopoietic system

abnormal positive T cell selection ( J:117684 )

• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice

• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology

decreased single-positive T cell number ( J:117684 )

• significant decrease in SP cell numbers

immune system

abnormal positive T cell selection ( J:117684 )

• only marginal increase in numbers of CD4 positive thymocytes is observed compared to TCR-transgenic mice

• CD4 SP/DP ratio is 0.29 compared to 0.46 in controls, indicating reduced positive selection

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated, double-positive thymocyte numbers compared to single transgenic littermates abnormal CD4 T cell morphology

decreased single-positive T cell number ( J:117684 )

• significant decrease in SP cell numbers

Genotype
MGI:3695390

cn47

• Allelic Composition: Lat2^tm2Wz/Lat2^tm2Wz; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6

immune system

enlarged spleen ( J:116157 )

• at 6 months of age

increased activated T cell number ( J:116157 )

• increased number of activated CD4 cells at 6 months of age

abnormal CD4-positive, alpha-beta T cell physiology ( J:116157 )

• increased production of IL-2, IL-10, and IFNG

increased anti-double stranded DNA antibody level ( J:116157 )

• high titers of dsDNA antibodies are present at 6 months of age

hematopoietic system

enlarged spleen ( J:116157 )

• at 6 months of age

increased activated T cell number ( J:116157 )

• increased number of activated CD4 cells at 6 months of age

abnormal CD4-positive, alpha-beta T cell physiology ( J:116157 )

• increased production of IL-2, IL-10, and IFNG

growth/size/body

enlarged spleen ( J:116157 )

• at 6 months of age

Genotype
MGI:3699363

cn48

• Allelic Composition: Ppp4c^tm1Tht/Ppp4c^tm1Tht; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

abnormal T cell differentiation ( J:117684 )

• developmental block specifically occurs at the double negative (DN) stage

• decreased percentage of TCR beta^hiCD3^hi mature thymocytes as well as decreased cell numbers are observed

abnormal positive T cell selection ( J:117684 )

• percentage of cells undergoing positive selection is decreased by ~60% compared to controls

• significant decrease in mature SP cell numbers

increased double-negative T cell number ( J:117684 )

• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block

decreased CD4-positive, alpha-beta T cell number ( J:117684 )

• significant decrease in numbers

decreased CD8-positive, alpha-beta T cell number ( J:117684 )

• significant decrease in numbers

decreased single-positive T cell number ( J:117684 )

• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes

abnormal T cell activation ( J:117684 )

• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed

hematopoietic system

abnormal T cell differentiation ( J:117684 )

• developmental block specifically occurs at the double negative (DN) stage

• decreased percentage of TCR beta^hiCD3^hi mature thymocytes as well as decreased cell numbers are observed

abnormal positive T cell selection ( J:117684 )

• percentage of cells undergoing positive selection is decreased by ~60% compared to controls

• significant decrease in mature SP cell numbers

increased double-negative T cell number ( J:117684 )

• numbers of double negative (DN) cells are comparable to controls; however higher percentage of DN cells result

decreased double-positive T cell number ( J:117684 )

• a significant decrease in unfractionated double-positive thymocyte numbers compared to Tg-negative littermates; 70% decrease is observed accompanying the developmental block

decreased CD4-positive, alpha-beta T cell number ( J:117684 )

• significant decrease in numbers

decreased CD8-positive, alpha-beta T cell number ( J:117684 )

• significant decrease in numbers

decreased single-positive T cell number ( J:117684 )

• numbers of CD3+, Cd4+, and CD8+ T cells are decreased by ~50-60% in spleen and lymph nodes

abnormal T cell activation ( J:117684 )

• antigen-specific T cell activation is decreased, with decreased proliferation in response to antigen observed

Genotype
MGI:3720606

cn49

• Allelic Composition: Fas^tm1Ach/Fas^tm1Ach; Tg(Lck-cre)548Jxm/?
• Genetic Background: involves: C57BL/6 * CBA

immune system

increased double-positive T cell number ( J:123556 )

• CD3+B220+ double positive cells accumulate

increased anti-nuclear antigen antibody level ( J:123556 )

hematopoietic system

increased double-positive T cell number ( J:123556 )

• CD3+B220+ double positive cells accumulate

Genotype
MGI:5544263

cn50

• Allelic Composition: Septin9^tm2.1Emfu/Septin9^tm2.1Emfu; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

decreased T cell proliferation ( J:205027 )

• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies

abnormal T cell differentiation ( J:205027 )

• partial at the DN3 to DN4 stage

decreased DN4 thymocyte number ( J:205027 )

increased double-negative T cell number ( J:205027 )

• in the thymus and lymph node

increased DN3 thymocyte number ( J:205027 )

increased CD8-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased T cell number ( J:205027 )

• general reduction in T cells

• more prominent reduction in CD8+ T cells compared with CD4+ T cells

decreased CD4-positive, alpha-beta T cell number ( J:205027 )

• reduced regulatory CD4+ T cells in the spleen

decreased CD4-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased CD8-positive, alpha-beta T cell number ( J:205027 )

• reduced cytotoxic CD8+ T cells in the spleen

decreased CD8-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased regulatory T cell number ( J:205027 )

• reduced regulatory CD4+ T cells in the spleen

• reduced CD3+ T cells

decreased single-positive T cell number ( J:205027 )

• most prominent in young mice

hematopoietic system

decreased T cell proliferation ( J:205027 )

• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies

abnormal T cell differentiation ( J:205027 )

• partial at the DN3 to DN4 stage

increased double-negative T cell number ( J:205027 )

• in the thymus and lymph node

increased DN3 thymocyte number ( J:205027 )

increased CD8-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased T cell number ( J:205027 )

• general reduction in T cells

• more prominent reduction in CD8+ T cells compared with CD4+ T cells

decreased DN4 thymocyte number ( J:205027 )

decreased CD4-positive, alpha-beta T cell number ( J:205027 )

• reduced regulatory CD4+ T cells in the spleen

decreased CD4-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased CD8-positive, alpha-beta T cell number ( J:205027 )

• reduced cytotoxic CD8+ T cells in the spleen

decreased CD8-positive, alpha-beta memory T cell number ( J:205027 )

• absolute numbers

decreased regulatory T cell number ( J:205027 )

• reduced regulatory CD4+ T cells in the spleen

• reduced CD3+ T cells

decreased single-positive T cell number ( J:205027 )

• most prominent in young mice

endocrine/exocrine glands

decreased DN4 thymocyte number ( J:205027 )

increased DN3 thymocyte number ( J:205027 )

cellular

decreased T cell proliferation ( J:205027 )

• in CD8+ T cells upon stimulation with anti-CD3 and anti-CD28 antibodies

Genotype
MGI:3836810

cn51

• Allelic Composition: Map3k3^tm1Bisu/Map3k3^tm2Bisu; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

decreased CD4-positive, alpha-beta T cell number ( J:145923 )

• CD4⁺ T cell numbers are reduced by about half in the spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:145923 )

• CD8⁺ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type

spleen hypoplasia ( J:145923 )

• spleen size is significantly reduced mostly due to a reduction in the number of T cells

abnormal T cell physiology ( J:145923 )

• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:145923 )

• CD4⁺ T cell numbers are reduced by about half in the spleen and lymph nodes

decreased CD8-positive, alpha-beta T cell number ( J:145923 )

• CD8⁺ T cell numbers in the spleen and lymph node are only 66% of that found in wild-type

spleen hypoplasia ( J:145923 )

• spleen size is significantly reduced mostly due to a reduction in the number of T cells

abnormal T cell physiology ( J:145923 )

• when transferred into a immunocompetent syngeneic host, mutant T cells fail to survive where as wild-type T cells do

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice

cellular

abnormal T cell proliferation ( J:145923 )

• homeostatic proliferation of T cells is defective as determined by competitive reconstitution assays with wild-type lymph node cells in sublethally irradiated hosts

• homeostatic proliferation is also defective in culture when mutant T cell proliferation is driven by the presence of syngeneic dendritc cells

• antigen driven proliferation is normal in these mice

Genotype
MGI:6719029

cn52

• Allelic Composition: Bcap31^tm1.1Bwang/Bcap31^tm1.1Bwang; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

increased thymocyte apoptosis ( J:274469 )

♀ • accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment

small thymus ( J:274469 )

♀ • decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)

decreased thymus weight ( J:274469 )

♀ • decreased thymus weight in young adult and older mice

thymus hypoplasia ( J:274469 )

♀

decreased thymocyte number ( J:274469 )

♀ • reduced total thymocyte number in young adult and older mice

♀ • however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal

♀ • no significant change in DN1-4 thymic subsets

decreased CD8-positive, naive alpha-beta T cell number ( J:274469 )

♀ • decreased % of naive (CD44^lowCD62L^hi) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)

increased effector memory T-helper cell number ( J:274469 )

♀ • increased % of effector/memory T cells (CD44^hiCD62L^lo) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)

decreased single-positive T cell number ( J:274469 )

♀ • decreased percentage of CD4+ and CD8+ single positive (SP) mature T lymphocytes in the spleen and lymph nodes of young adult (3-, 5- or 8-week-old) and older mice (6-month-old)

♀ • decreased percentage of CD4+ and CD8+ T cells in peripheral blood

♀ • however, expression of class I MHC molecules on CD4+ and CD8+ SP splenocytes is normal

abnormal T cell activation ( J:274469 )

♀ • marked reduction in IL-2 and IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28

decreased T cell proliferation ( J:274469 )

♀ • reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28

decreased interferon-gamma secretion ( J:274469 )

♀ • marked reduction in IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28; also detected at the mRNA level

decreased interleukin-2 secretion ( J:274469 )

• marked reduction in IL-2 production following stimulation of total splenocytes with anti-CD3 plus anti-CD28; also detected at the mRNA level

hematopoietic system

increased thymocyte apoptosis ( J:274469 )

♀ • accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment

small thymus ( J:274469 )

♀ • decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)

decreased thymus weight ( J:274469 )

♀ • decreased thymus weight in young adult and older mice

thymus hypoplasia ( J:274469 )

♀

decreased thymocyte number ( J:274469 )

♀ • reduced total thymocyte number in young adult and older mice

♀ • however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal

♀ • no significant change in DN1-4 thymic subsets

decreased CD8-positive, naive alpha-beta T cell number ( J:274469 )

♀ • decreased % of naive (CD44^lowCD62L^hi) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)

increased effector memory T-helper cell number ( J:274469 )

♀ • increased % of effector/memory T cells (CD44^hiCD62L^lo) in CD4+ and CD8+ T cells in the spleen and lymph nodes of young adult (8-wk-old) and older mice (6-mo-old)

decreased single-positive T cell number ( J:274469 )

♀ • decreased percentage of CD4+ and CD8+ single positive (SP) mature T lymphocytes in the spleen and lymph nodes of young adult (3-, 5- or 8-week-old) and older mice (6-month-old)

♀ • decreased percentage of CD4+ and CD8+ T cells in peripheral blood

♀ • however, expression of class I MHC molecules on CD4+ and CD8+ SP splenocytes is normal

abnormal T cell activation ( J:274469 )

♀ • marked reduction in IL-2 and IFN-gamma production following stimulation of total splenocytes with anti-CD3 plus anti-CD28

decreased T cell proliferation ( J:274469 )

♀ • reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28

cellular

increased thymocyte apoptosis ( J:274469 )

♀ • accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment

decreased T cell proliferation ( J:274469 )

♀ • reduced T cell proliferation following stimulation of total splenocytes with anti-CD3 plus anti-CD28

endocrine/exocrine glands

increased thymocyte apoptosis ( J:274469 )

♀ • accelerated apoptosis in freshly isolated thymocytes after tunicamycin treatment

small thymus ( J:274469 )

♀ • decreased thymus size in young adult (3-, 5- or 8-week-old) and older mice (6-month-old)

decreased thymus weight ( J:274469 )

♀ • decreased thymus weight in young adult and older mice

thymus hypoplasia ( J:274469 )

♀

decreased thymocyte number ( J:274469 )

♀ • reduced total thymocyte number in young adult and older mice

♀ • however, development of double-negative (DN) thymocytes and CD4+ and CD8+ single-positive (SP) thymocytes is normal

♀ • no significant change in DN1-4 thymic subsets

Genotype
MGI:3696482

cn53

• Allelic Composition: Gt(ROSA)26Sor^tm1Hjf/?; Tg(Lck-cre)548Jxm/0
• Genetic Background: involves: C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected ( J:117041 )

• mice are viable; strong variation in percentage of RFP-positive T lymphocytes is observed between individual mice, as well as activation in non-T lineage cells