All Phenotypes Tg(Col2a1-cre)3Amc MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(Col2a1-cre)3Amc
transgene insertion 3, Andrew P McMahon
MGI:2176263

Summary

8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor^{tm3(Runx2)Flng} Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0	involves: 129S1/Sv * 129X1/SvJ	MGI:5311114
cn2	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Col2a1-cre)3Amc/0	involves: 129S1/Sv * 129X1/SvJ	MGI:3689414
cn3	Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0	involves: 129S1/Sv * 129X1/SvJ	MGI:4414674
cn4	Gli3^Xt-J/Gli3^Xt-J Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0	involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ	MGI:4414675
cn5	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor^{tm3(Runx2)Flng} Runx2^tm1Mjo/Runx2^tm1Mjo Tg(Col2a1-cre)3Amc/0	involves: 129X1/SvJ	MGI:5311112
cn6	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor⁺ Runx2^tm1Mjo/Runx2^tm1Mjo Tg(Col2a1-cre)3Amc/0	involves: 129X1/SvJ	MGI:5311113
cn7	Smo^tm1Amc/Smo^tm2Amc Tg(Col2a1-cre)3Amc/0	involves: 129X1/SvJ	MGI:3584114
cn8	Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Tg(Col2a1-cre)3Amc/0	involves: 129X1/SvJ	MGI:4414673

Allelic Composition	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor^{tm3(Runx2)Flng} Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm3(Runx2)Flng} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
Ihh^tm1Amc mutation (1 available); any Ihh mutation (22 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

abnormal long bone epiphyseal plate morphology ( J:180304 )

• the nonhypertrophic chondrocyte zone in the tibia is reduced to a greater extent than in Ihh^tm1Amc homozygotes

abnormal long bone hypertrophic chondrocyte zone ( J:180304 )

• hypertrophic chondrocytes exhibit accelerated progression from early stage to late stage hypertrophy compared with control mice

abnormal bone structure ( J:180304 )

• thin bone collar

abnormal osteoblast differentiation ( J:180304 )

• impaired

abnormal bone ossification ( J:180304 )

• impaired

cellular

abnormal osteoblast differentiation ( J:180304 )

• impaired

Allelic Composition	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ctnnb1^tm2Kem mutation (1 available); any Ctnnb1 mutation (49 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

abnormal long bone morphology ( J:114494 )

• at E18.5, no identifiable bone matrix is observed in long bones

Allelic Composition	Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm2(Gli2*)Flng} mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Ihh^tm1Amc mutation (1 available); any Ihh mutation (22 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:154905 )

• mice die at birth

skeleton

skeleton phenotype ( J:154905 )

N	• vascularization and bone formation in the diaphysis are normal

abnormal osteoblast differentiation ( J:154905 )

• similar to in Ihh^tm1Amc homozygotes, orthotopic osteoblast differentiation is impaired as determined by marker expression

abnormal long bone epiphyseal plate proliferative zone ( J:154905 )

• at E15.5 and E18.5, the proliferative zone is slightly larger than in Ihh^tm1Amc homozygotes but smaller than in wild-type mice

abnormal long bone hypertrophic chondrocyte zone ( J:154905 )

• vascularization of the hypertrophic zone is improved compared to in Ihh^tm1Amc homozygotes

disorganized long bone epiphyseal plate ( J:154905 )

• columnar organization of chondrocytes is partially restored compared to in Ihh^tm1Amc homozygotes but is still disorganized compared to in wild-type mice

abnormal perichondrium morphology ( J:154905 )

• at E18.5, mice fail to exhibit bone deposition in the perichondrium flanking the hypertrophic regions where the bone collar normally forms in the long bones of wild-type mice

cellular

abnormal osteoblast differentiation ( J:154905 )

• similar to in Ihh^tm1Amc homozygotes, orthotopic osteoblast differentiation is impaired as determined by marker expression

Allelic Composition	Gli3^Xt-J/Gli3^Xt-J Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Ihh^tm1Amc/Ihh^tm1Amc Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gli3^Xt-J mutation (3 available); any Gli3 mutation (81 available)
Gt(ROSA)26Sor^{tm2(Gli2*)Flng} mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Ihh^tm1Amc mutation (1 available); any Ihh mutation (22 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:154905 )

• mice die at birth

skeleton

skeleton phenotype ( J:154905 )

N	• unlike in Ihh^tm1Amc Gli3^Xt-J homozygotes the marrow cavity and hypertrophic chondrocyte are normal

abnormal long bone diaphysis morphology ( J:154905 )

• the growth region cartilage is longer than in wild-type mice

• the columnar zone contains areas of disorganization unlike in wild-type mice

• however, orthotopic bone collar formation is normal unlike in Ihh^tm1Amc homozygotes

growth/size/body

decreased body size ( J:154905 )

• while larger than Ihh^tm1Amc homozygotes at E18.5, mice are smaller than wild-type mice

limbs/digits/tail

short limbs ( J:154905 )

• while larger than in Ihh^tm1Amc homozygotes at E18.5, limbs are shorter than in wild-type mice

Allelic Composition	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor^{tm3(Runx2)Flng} Runx2^tm1Mjo/Runx2^tm1Mjo Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm3(Runx2)Flng} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
Runx2^tm1Mjo mutation (0 available); any Runx2 mutation (44 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

skeleton phenotype ( J:180304 )

N	• unlike Runx2^tm1Mjo homozygotes, mice exhibit normal cartilage, bone collar, and marrow and restored osteoblast differentiation and cartilage hypertrophy

abnormal bone ossification ( J:180304 )

• at E18.5, bone formation defects observed in Runx2^tm1Mjo homozygotes are partially recovered in the endochondrial skeleton but not the skull

Allelic Composition	Gt(ROSA)26Sor^{tm3(Runx2)Flng}/Gt(ROSA)26Sor⁺ Runx2^tm1Mjo/Runx2^tm1Mjo Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm3(Runx2)Flng} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
Runx2^tm1Mjo mutation (0 available); any Runx2 mutation (44 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

skeleton phenotype ( J:180304 )

N	• unlike Runx2^tm1Mjo homozygotes, mice exhibit normal bone collar

abnormal bone marrow cavity morphology ( J:180304 )

• like Runx2^tm1Mjo homozygotes, mice lack a marrow cavity

abnormal bone ossification ( J:180304 )

• at E18.5, bone formation defects observed in Runx2^tm1Mjo homozygotes are partially recovered in the endochondrial skeleton but not the skull

Allelic Composition	Smo^tm1Amc/Smo^tm2Amc Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129X1/SvJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smo^tm1Amc mutation (1 available); any Smo mutation (39 available)
Smo^tm2Amc mutation (1 available); any Smo mutation (39 available)
Tg(Col2a1-cre)3Amc mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

decreased length of long bones ( J:73071 )

• shorter long bones, more severe than in mutants carrying Tg(Col2a1-cre)15Amc but less severe than in mutants carrying Tg(Col2a1-cre)10Amc

Allelic Composition	Gt(ROSA)26Sor^{tm2(Gli2*)Flng}/Gt(ROSA)26Sor⁺ Tg(Col2a1-cre)3Amc/0
Genetic Background	involves: 129X1/SvJ

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

skeleton

decreased length of long bones ( J:154905 )

• 10% to 15% at E18.5

abnormal cartilage development ( J:154905 )

• as determined by marker expression, cartilage growth in long bones is reduced due to premature onset of hypertrophy compared with wild-type mice

abnormal long bone epiphyseal plate proliferative zone ( J:154905 )

• decreased at E14.5 and E18.5

decreased width of hypertrophic chondrocyte zone ( J:154905 )

• at E18.5

increased width of hypertrophic chondrocyte zone ( J:154905 )

• at E14.5

growth/size/body

decreased body size ( J:154905 )

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