Phenotypes associated with this allele

• Allele Symbol: Mpo^tm1Mae
• Allele Name: targeted mutation 1, Nobuyo Maeda
• Allele ID: MGI:2176344
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mpo^tm1Mae/Mpo^tm1Mae	involves: 129P2/OlaHsd * C57BL/6	MGI:2176346

Genotype
MGI:2176346

hm1

• Allelic Composition: Mpo^tm1Mae/Mpo^tm1Mae
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal neutrophil physiology ( J:53993 )

• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity

• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2^- generation than those of wild-type mice

increased susceptibility to fungal infection ( J:53993 )

• homozygotes are viable, fertile and developmentally normal with normal clearance of intraperitoneal S. aureus by 48 hrs post-infection

• however, homozygotes show increased susceptibility to intratracheal infection with C. albicans, with severe alveolar and peribronchiolar pneumonia resulting in death of two-thirds of mutants by 5 days post-infection even at a relatively low dose (4 10⁶ fungi/mouse)

• when challenged intraperitoneally at a similar dose (4 10⁶ fungi/mouse), both wild-type and mutant mice show no obvious signs of severe infection at 7 days after challenge, despite significant fungal dissemination in various mutant organs, with lungs and kidneys being most severely affected

• at a higher i.p. dose (10⁸ fungi/mouse), all homozygotes die by 2 days, whereas all wild-type mice survive for 7 days without many signs of distress

homeostasis/metabolism

abnormal metabolism ( J:53993 )

• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity

• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2^- generation than those of wild-type mice

hematopoietic system

abnormal neutrophil physiology ( J:53993 )

• as expected, PMA-stimulated peritoneal exudate neutrophils from homozygotes fail to generate hypochlorous acid (HOCl) from hydrogen peroxide, due to absence of peroxidase activity

• also, peritoneal exudate neutrophils from homozygotes show a slightly higher level of O2^- generation than those of wild-type mice