Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-cat,-lacZ)11Miya
• Allele Name: transgene insertion 11, Jun-ichi Miyazaki
• Allele ID: MGI:2176512
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Col1a1^tm1(tetO-EWSR1/ATF1)Yasu/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+ H2az2^Tg(Wnt1-cre)11Rth/H2az2^+ Tg(CAG-cat,-lacZ)11Miya/0	involves: 129S4/SvJae * C57BL/6 * CBA/J * DBA/2	MGI:5485200
cn2	Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat,-lacZ)11Miya/0 Tg(Pomc1-cre)16Lowl/0	involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N	MGI:4820873
cn3	Gt(ROSA)26Sor^tm1(Foxo1)Jnk/Gt(ROSA)26Sor^+ Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat,-lacZ)11Miya/0 Tg(Pomc1-cre)16Lowl/0	involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N	MGI:4820871
cn4	Gt(ROSA)26Sor^tm2(Foxo1)Jnk/Gt(ROSA)26Sor^+ Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat,-lacZ)11Miya/0 Tg(Pomc1-cre)16Lowl/0	involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N	MGI:4820872

Genotype
MGI:5485200

cn1

• Allelic Composition: Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺; Tg(CAG-cat,-lacZ)11Miya/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA/J * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased sarcoma incidence ( J:194505 )

• in of all doxycycline-treated mice arising from neural crest-lineage cells

Genotype
MGI:4820873

cn2

• Allelic Composition: Pdpk1^tm1Maka/Pdpk1^tm1Maka; Tg(CAG-cat,-lacZ)11Miya/0; Tg(Pomc1-cre)16Lowl/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N

nervous system

increased pituitary gland apoptosis ( J:162892 )

• mice exhibit increased apoptosis in the pituitary compared with wild-type mice

increased corticotroph apoptosis ( J:162892 )

• mice exhibit a decrease in corticotrophs in the anterior pituitary due to apoptosis unlike in wild-type mice

• however, corticotroph development in the anterior pituitary at E18.5 is normal

increased melanotroph apoptosis ( J:162892 )

• mice exhibit a decrease in melanotrophs in the intermediate pituitary due to apoptosis unlike in wild-type mice

• however, melanotrophs development in the intermediate pituitary at E18.5 is normal

abnormal pituitary intermediate lobe morphology ( J:162892 )

• extremely thin

abnormal neuron physiology ( J:162892 )

• Pomc1 neurons exhibit a blunted influx of calcium ions in response to leptin compared with similarly treated wild-type neurons

homeostasis/metabolism

hypoglycemia ( J:162892 )

decreased circulating corticosterone level ( J:162892 )

• at 16 weeks in fed and fasted states

increased insulin sensitivity ( J:162892 )

decreased response to leptin ( J:162892 )

• following intraperitoneal leptin injection, mice exhibit a lesser decrease in body weight and food intake compared with similarly treated wild-type mice

adipose tissue

increased total body fat amount ( J:162892 )

• especially visceral fat mass

behavior/neurological

increased food intake ( J:162892 )

• food intake is increased compared to in wild-type mice

endocrine/exocrine glands

increased pituitary gland apoptosis ( J:162892 )

• mice exhibit increased apoptosis in the pituitary compared with wild-type mice

increased corticotroph apoptosis ( J:162892 )

• mice exhibit a decrease in corticotrophs in the anterior pituitary due to apoptosis unlike in wild-type mice

• however, corticotroph development in the anterior pituitary at E18.5 is normal

increased melanotroph apoptosis ( J:162892 )

• mice exhibit a decrease in melanotrophs in the intermediate pituitary due to apoptosis unlike in wild-type mice

• however, melanotrophs development in the intermediate pituitary at E18.5 is normal

abnormal pituitary intermediate lobe morphology ( J:162892 )

• extremely thin

cellular

increased pituitary gland apoptosis ( J:162892 )

• mice exhibit increased apoptosis in the pituitary compared with wild-type mice

increased corticotroph apoptosis ( J:162892 )

• mice exhibit a decrease in corticotrophs in the anterior pituitary due to apoptosis unlike in wild-type mice

• however, corticotroph development in the anterior pituitary at E18.5 is normal

increased melanotroph apoptosis ( J:162892 )

• mice exhibit a decrease in melanotrophs in the intermediate pituitary due to apoptosis unlike in wild-type mice

• however, melanotrophs development in the intermediate pituitary at E18.5 is normal

Genotype
MGI:4820871

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Foxo1)Jnk}/Gt(ROSA)26Sor⁺; Pdpk1^tm1Maka/Pdpk1^tm1Maka; Tg(CAG-cat,-lacZ)11Miya/0; Tg(Pomc1-cre)16Lowl/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N

growth/size/body

increased body weight ( J:162892 )

• mice a tend towards increased in body weight compared with Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat-lacZ)11Miya Tg(Pomc1-cre)16Lowl mice

Genotype
MGI:4820872

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm2(Foxo1)Jnk}/Gt(ROSA)26Sor⁺; Pdpk1^tm1Maka/Pdpk1^tm1Maka; Tg(CAG-cat,-lacZ)11Miya/0; Tg(Pomc1-cre)16Lowl/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2 * FVB/N

adipose tissue

increased total body fat amount ( J:162892 )

• female mice exhibit a tended towards increased adiposity compared with wild-type mice

behavior/neurological

increased food intake ( J:162892 )

• mice exhibit increased food intake compared with wild-type mice and Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat-lacZ)11Miya Tg(Pomc1-cre)16Lowl mice

growth/size/body

increased body weight ( J:162892 )

• at 16 weeks, mice are significantly heavier than Pdpk1^tm1Maka/Pdpk1^tm1Maka Tg(CAG-cat-lacZ)11Miya Tg(Pomc1-cre)16Lowl mice