Analysis Tools
normal phenotype
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• mice are viable; dual-reporter mice were used for detailed profiling of Il4 mRNA and protein production in response to stimulation in culture and parasitic infection in vivo
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Allele Symbol Allele Name Allele ID |
Il4tm1Lky targeted mutation 1, Richard M Locksley MGI:2176574 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are viable; dual-reporter mice were used for detailed profiling of Il4 mRNA and protein production in response to stimulation in culture and parasitic infection in vivo
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the first CD4+ T cells that seed the periphery in newborn mice undergo rapid expansion and convert into pathogenic Th2 effector cells
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• the first CD4+ T cells that seed the periphery in newborn mice undergo rapid expansion and convert into pathogenic Th2 effector cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• both Th1 and Th2 programs can be induced in Itk-null cells when Th1 priming or Th2 priming conditions are used
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• upon restimulation with anti-TCR antibodies, numbers of Il4 (EGFP) expressing cells remains constant (~65%, elevated relative to wild-type cells) but Il4 protein production is markedly attenuated; however if TCR-mediated signaling is bypassed with stimulation by PMA/ionomycin, Th2-primed null cells show similar levels of Il4 secretion to wild-type cells
• similar results are obtained in vivo
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• only Th1-primed cells can secrete effector cytokines (interferon gamma) upon restimulation; Th2-primed cells do not secrete Il4 upon restimulation as shown by Il4 reporter expression
• similar results are obtained in vivo
• after infection with Leishmania major, restimulation with L. major antigens reveals defects in Il4 production
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• after infection with Leishmania major, restimulation with L. major antigens reveals defects in Il4 production
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• fewer Itk-null lymphocytes undergo activation induced cell death than wild-type cells upon restimulation
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• upon restimulation with anti-TCR antibodies, numbers of Il4 (EGFP) expressing cells remains constant (~65%, elevated relative to wild-type cells) but Il4 protein production is markedly attenuated; however if TCR-mediated signaling is bypassed with stimulation by PMA/ionomycin, Th2-primed null cells show similar levels of Il4 secretion to wild-type cells
• similar results are obtained in vivo
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 06/12/2024 MGI 6.13 |
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