All Phenotypes Il6st<tm1Kish> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Il6st^tm1Kish/Il6st^tm1Kish	involves: 129P2/OlaHsd * C57BL/6	MGI:2176788
hm2	Il6st^tm1Kish/Il6st^tm1Kish	involves: 129P2/OlaHsd * CD-1	MGI:4352710
hm3	Il6st^tm1Kish/Il6st^tm1Kish	involves: 129P2/OlaHsd * ICR	MGI:3852648

Allelic Composition	Il6st^tm1Kish/Il6st^tm1Kish
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il6st^tm1Kish mutation (0 available); any Il6st mutation (80 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:75419 )

• embryos start dying around E12.5 with less than 3% of embryos being alive at E18.5

• no homozygote pups are born alive

cardiovascular system

thin ventricular wall ( J:75419 )

• E16.5 embryos have extremely thin ventricular walls that are only one cell thick

• E15.5 embryos have mostly normal ventricular walls though half have some ventricular thinness (30% reduction)

embryo

decreased embryo size ( J:75419 )

• half of the live E13.5 embryos are 10-20% smaller than controls

hematopoietic system

thymus hypoplasia ( J:75419 )

• the thymus of E16.5 embryos is about 50% smaller than controls

decreased common myeloid progenitor cell number ( J:75419 )

• CFU-S and CFU-GM cells are reduced in the E13.5 embryo

anemia ( J:75419 )

• 20% of E15.5-E18.5 embryos are pale in appearance

decreased erythroid progenitor cell number ( J:75419 )

• the number of BFU-E cells found in the E13.5 embryo is severely reduced compared to controls

increased nucleated erythrocyte cell number ( J:75419 )

• pale embryos still have the presence of nucleated red blood cells

decreased hematopoietic stem cell number ( J:75419 )

• the number of mononuclear cells and CFU stem cells found in the E13.5 embryo is severely reduced compared to controls

immune system

thymus hypoplasia ( J:75419 )

• the thymus of E16.5 embryos is about 50% smaller than controls

growth/size/body

decreased embryo size ( J:75419 )

• half of the live E13.5 embryos are 10-20% smaller than controls

endocrine/exocrine glands

thymus hypoplasia ( J:75419 )

• the thymus of E16.5 embryos is about 50% smaller than controls

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:88710 )

• mice die within one day of birth

growth/size/body

decreased body size ( J:88710 )

• fetuses appear normal except that they are smaller and have shorter limbs

endocrine/exocrine glands

increased activity of parathyroid ( J:88710 )

• low serum calcium and high PTH levels in these mice is indicative of secondary hyperparathyroidism

skeleton

abnormal osteoblast physiology ( J:88710 )

• osteoblasts have decreased levels of alkaline phosphatase activity

• calvariae osteoblasts cultured in vitro are unable to form bony nodules as wild-type controls are

abnormal osteoclast physiology ( J:88710 )

• osteoblasts have decreased levels of alkaline phosphatase activity

• calvariae osteoblasts cultured in vitro are unable to form bony nodules as wild-type controls are

abnormal skeleton morphology ( J:88710 )

• fetal skeleton is noticeably smaller

abnormal osteoclast morphology ( J:88710 )

abnormal osteoclast differentiation ( J:88710 )

• osteoclasts are larger and more ovoid in shape when compared to wild-type controls

• the ruffled borders of mutant osteoclasts are thicker, blunter, and fewer in number

• the number of folds in the ruffled border is reduced by 6-fold or more

increased osteoclast cell number ( J:88710 )

• osteoclasts number and percentage of osteoclast surface per bone surface is significantly increased at both the metaphysic and diaphysis of bone

abnormal long bone morphology ( J:88710 )

bowed humerus ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed radius ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed ulna ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed femur ( J:88710 )

• femur of E18.5 fetuses is bowed

bowed fibula ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed tibia ( J:88710 )

• long bones of E17.5 fetuses are bowed

abnormal long bone diaphysis morphology ( J:88710 )

• well-formed marrow space and diaphysis with scattered thin spicules of primary trabecular bone does not occur until E17.5 compared to E16.5 for controls

• eccentric thickening of the diaphyseal cortex also occurs during development

decreased osteoblast cell number ( J:88710 )

• osteoblast number and surface area are significantly reduced in the tibia by more than half

abnormal trabecular bone morphology ( J:88710 )

• mice have poor development of the spongy bone material in the bone marrow

limbs/digits/tail

bowed humerus ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed radius ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed ulna ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed femur ( J:88710 )

• femur of E18.5 fetuses is bowed

bowed fibula ( J:88710 )

• long bones of E17.5 fetuses are bowed

bowed tibia ( J:88710 )

• long bones of E17.5 fetuses are bowed

short limbs ( J:88710 )

• fetuses appear normal except that they are smaller and have shorter limbs

immune system

abnormal osteoclast morphology ( J:88710 )

abnormal osteoclast differentiation ( J:88710 )

• osteoclasts are larger and more ovoid in shape when compared to wild-type controls

• the ruffled borders of mutant osteoclasts are thicker, blunter, and fewer in number

• the number of folds in the ruffled border is reduced by 6-fold or more

increased osteoclast cell number ( J:88710 )

• osteoclasts number and percentage of osteoclast surface per bone surface is significantly increased at both the metaphysic and diaphysis of bone

abnormal osteoclast physiology ( J:88710 )

• osteoblasts have decreased levels of alkaline phosphatase activity

• calvariae osteoblasts cultured in vitro are unable to form bony nodules as wild-type controls are

abnormal bone marrow development ( J:88710 )

• there is a relative paucity of bone marrow in E18.5 tibias compared to controls

homeostasis/metabolism

increased circulating parathyroid hormone level ( J:88710 )

• mice have an increased mean circulating PTH level of 239 pg /ml compared to 20.3 pg/ml in controls

decreased circulating calcium level ( J:88710 )

• the mean 1.44 mmol/liter serum calcium of E18.5 embryos is significantly less than the 1.73 mmol/liter in controls

hematopoietic system

abnormal osteoclast morphology ( J:88710 )

abnormal osteoclast differentiation ( J:88710 )

• osteoclasts are larger and more ovoid in shape when compared to wild-type controls

• the ruffled borders of mutant osteoclasts are thicker, blunter, and fewer in number

• the number of folds in the ruffled border is reduced by 6-fold or more

increased osteoclast cell number ( J:88710 )

• osteoclasts number and percentage of osteoclast surface per bone surface is significantly increased at both the metaphysic and diaphysis of bone

abnormal osteoclast physiology ( J:88710 )

• osteoblasts have decreased levels of alkaline phosphatase activity

• calvariae osteoblasts cultured in vitro are unable to form bony nodules as wild-type controls are

cellular

abnormal osteoclast differentiation ( J:88710 )

• osteoclasts are larger and more ovoid in shape when compared to wild-type controls

• the ruffled borders of mutant osteoclasts are thicker, blunter, and fewer in number

• the number of folds in the ruffled border is reduced by 6-fold or more

abnormal osteoblast physiology ( J:88710 )

• osteoblasts have decreased levels of alkaline phosphatase activity

• calvariae osteoblasts cultured in vitro are unable to form bony nodules as wild-type controls are

Allelic Composition	Il6st^tm1Kish/Il6st^tm1Kish
Genetic Background	involves: 129P2/OlaHsd * ICR

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il6st^tm1Kish mutation (0 available); any Il6st mutation (80 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality ( J:54660 )

• Background Sensitivity: embryos on an outbred ICR background often survive until birth

homeostasis/metabolism

decreased liver glycogen level ( J:54660 )

• accumulation of intracellular glycogen is greatly reduced in the liver of E17.5 and newborn mice

liver/biliary system

decreased liver glycogen level ( J:54660 )

• accumulation of intracellular glycogen is greatly reduced in the liver of E17.5 and newborn mice

nervous system

abnormal cortical plate morphology ( J:124527 )

• cellularity of the cortical plate in E15 embryos is reduced about a third compared to controls

• this reduced numbers are found in anterior, intermediate, and posterior parts of the developing cortex

• the proliferative rate of the cortical plate neurons is significantly decreased compared to controls by about 40%

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