Phenotypes associated with this allele

• Allele Symbol: Fus^{Gt(U3NeoSV1)HO14Ggh}
• Allele Name: gene trap HO14, Geoffrey G Hicks
• Allele ID: MGI:2177561
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fus^Gt(U3NeoSV1)HO14Ggh/Fus^Gt(U3NeoSV1)HO14Ggh	B6.129S2-Fus^Gt(U3NeoSV1)HO14Ggh	MGI:2177564
hm2	Fus^Gt(U3NeoSV1)HO14Ggh/Fus^Gt(U3NeoSV1)HO14Ggh	involves: 129S2/SvPas	MGI:3663103

Genotype
MGI:2177564

hm1

• Allelic Composition: Fus^{Gt(U3NeoSV1)HO14Ggh}/Fus^{Gt(U3NeoSV1)HO14Ggh}
• Genetic Background: B6.129S2-Fus^{Gt(U3NeoSV1)HO14Ggh}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:60213 )

• pups fail to suckle and die within 16 hours of birth

cellular

abnormal chromosome morphology ( J:60213 )

• centromeric fusion and presence of extrachromosomal elements are frequently observed

aneuploidy ( J:60213 )

• E14.5 metaphase Fus-deficient chromosomes show aneuploidy in more than 67% of cells examined

chromosome breakage ( J:60213 )

• this is frequently observed in Fus-deficient cells

behavior/neurological

abnormal suckling behavior ( J:60213 )

• newborns fail to suckle

hematopoietic system

small thymus ( J:60213 )

• thymus is reduced in size in mutants from E16 to birth

decreased lymphocyte cell number ( J:60213 )

• white blood cell numbers are reduced in homozygotes and lymphocytes account for almost all of the reduction

decreased B cell number ( J:60213 )

• there is a reduction in the number of IgM+ B cells in the spleen

abnormal immunoglobulin level ( J:60213 )

• after transfer of Fus-deficient fetal liver cells to irradiated wild-type hosts, levels of IgM produced in response to OVA challenges are 9.8-fold lower compared to hosts receiving wild-type liver cells

immune system

small thymus ( J:60213 )

• thymus is reduced in size in mutants from E16 to birth

decreased lymphocyte cell number ( J:60213 )

• white blood cell numbers are reduced in homozygotes and lymphocytes account for almost all of the reduction

decreased B cell number ( J:60213 )

• there is a reduction in the number of IgM+ B cells in the spleen

abnormal immunoglobulin level ( J:60213 )

• after transfer of Fus-deficient fetal liver cells to irradiated wild-type hosts, levels of IgM produced in response to OVA challenges are 9.8-fold lower compared to hosts receiving wild-type liver cells

lymphoid hypoplasia ( J:60213 )

• mice have reduced cellularity in lymphoid organs

endocrine/exocrine glands

small thymus ( J:60213 )

• thymus is reduced in size in mutants from E16 to birth

Genotype
MGI:3663103

hm2

• Allelic Composition: Fus^{Gt(U3NeoSV1)HO14Ggh}/Fus^{Gt(U3NeoSV1)HO14Ggh}
• Genetic Background: involves: 129S2/SvPas

nervous system

abnormal hippocampus pyramidal cell morphology ( J:97092 )

• cultured neurons from Fus-deficient mice have irregularly branched dendrites and numerous long thin processes like immature axons extended from the cell body; neurons possess a single axon and multiple dendrites

• numbers of spines are reduced or transformed into long, thin filamentous protrusions resembling filopodia

• there are more spine branches in proximal and distal regions compared to wild-type; there are more tertiary dendritic branches in the distal region