Phenotypes associated with this allele

• Allele Symbol: Nlk^tm1Tbo
• Allele Name: targeted mutation 1, Thomas Boehm
• Allele ID: MGI:2177606
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nlk^tm1Tbo/Nlk^tm1Tbo	129S1/Sv-Nlk^tm1Tbo	MGI:2673115
hm2	Nlk^tm1Tbo/Nlk^tm1Tbo	involves: 129S1/Sv * C57BL/6	MGI:2673113

Genotype
MGI:2673115

hm1

• Allelic Composition: Nlk^tm1Tbo/Nlk^tm1Tbo
• Genetic Background: 129S1/Sv-Nlk^tm1Tbo

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:73353 )

• Background Sensitivity: animals on the 129S1/Sv background survive up to 4-6 weeks of age, longer than on a mixed 129S1/Sv and C57BL/6 background

behavior/neurological

ataxia ( J:73353 )

• animals exhibit a pronounced cerebellar ataxia

growth/size/body

postnatal growth retardation ( J:73353 )

hematopoietic system

thymus hypoplasia ( J:73353 )

• hypocellular thymus; mean cellularity is reduced by 70%

abnormal definitive hematopoiesis ( J:73353 )

decreased pro-B cell number ( J:73353 )

• pro-B cells are reduced by 60-80%; however, the function of the remaining mature cells appears normal

impaired myelopoiesis ( J:73353 )

• reduced numbers of myeloid cells

decreased bone marrow cell number ( J:73353 )

• total cellularity of bone marrow is reduced by about half

• lower ratio of lymphoid vs. non-lymphoid nucleated cells in the bone marrow

decreased lymphocyte cell number ( J:73353 )

decreased thymocyte number ( J:73353 )

• significant loss of cortical thymocytes

decreased double-positive T cell number ( J:73353 )

decreased NK cell number ( J:73353 )

• reduction in NK cells in the thymus

decreased gamma-delta T cell number ( J:73353 )

• reduction in the thymus

spleen hypoplasia ( J:73353 )

• hypocellular spleen; mean cellularity is reduced by 90%

immune system

decreased pro-B cell number ( J:73353 )

• pro-B cells are reduced by 60-80%; however, the function of the remaining mature cells appears normal

impaired myelopoiesis ( J:73353 )

• reduced numbers of myeloid cells

decreased lymphocyte cell number ( J:73353 )

decreased double-positive T cell number ( J:73353 )

decreased NK cell number ( J:73353 )

• reduction in NK cells in the thymus

decreased gamma-delta T cell number ( J:73353 )

• reduction in the thymus

abnormal immune system organ morphology ( J:73353 )

thymus hypoplasia ( J:73353 )

• hypocellular thymus; mean cellularity is reduced by 70%

decreased thymocyte number ( J:73353 )

• significant loss of cortical thymocytes

spleen hypoplasia ( J:73353 )

• hypocellular spleen; mean cellularity is reduced by 90%

abnormal bone marrow development ( J:73353 )

• abnormal differentiation of bone marrow stroma, showing variable increases in adipogenesis, abnormal sinus structures and sometimes lack of bone-lining cells

small lymph nodes ( J:73353 )

endocrine/exocrine glands

thymus hypoplasia ( J:73353 )

• hypocellular thymus; mean cellularity is reduced by 70%

decreased thymocyte number ( J:73353 )

• significant loss of cortical thymocytes

Genotype
MGI:2673113

hm2

• Allelic Composition: Nlk^tm1Tbo/Nlk^tm1Tbo
• Genetic Background: involves: 129S1/Sv * C57BL/6

mortality/aging

prenatal lethality, complete penetrance ( J:73353 )

• Background Sensitivity: mutants on a mixed 129S1/Sv and C57BL/6 background die in the third trimester of pregnancy, sooner than mutants on a 129S1/Sv background