All Phenotypes Fn1<tm2Hyn> MGI Mouse

embryonic lethality during organogenesis, complete penetrance ( J:36210 )

• homozygotes start developing severe embryonic abnormalities at ~E8.0 and appear significantly distorted at E9.5

• by E9.5, some homozygotes have undergone complete degeneration; no mutant embryos are detected after E10

absent vitelline blood vessels ( J:36210 )

• at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac

short rostral-caudal axis ( J:36210 )

• at E8.5, homozygotes display a shortened anterior-posterior axis

embryonic growth retardation ( J:36210 )

• at E8.0-E8.5, homozygotes are clearly retarded in development relative to wild-type embryos

head mesenchyme hypoplasia ( J:36210 )

• at E8.0, homozygotes show a significant reduction in head mesenchyme and develop defects in mesodermal derivatives (i.e. heart, yolk sac vasculature, notochord and somites)

kinked neural tube ( J:36210 )

• at E8.5, homozygotes display a kinked neural tube

abnormal notochord morphology ( J:36210 )

• at E8.5, homozygotes lack an organized notochord, despite normal initial production, determination and migration of notochord precursor cells

absent somites ( J:36210 )

• at E8.5, homozygotes display absence of somites: cells expressing presomitic and somitic markers are detected at the correct locations but fail to organize as somites

abnormal amnion morphology ( J:36210 )

• at E7.5, homozygotes possess all three germ layers, including a mesoderm, but exhibit a concave amnion

abnormal visceral yolk sac morphology ( J:36210 )

• at E8.5, the mesodermal and endodermal layers of the mutant yolk sac appear to split apart