Analysis Tools
mortality/aging
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• a significant portion of homozygotes survive to E16.5; however, no live homozygotes are recovered at E18.5
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• a portion of homozygotes die between E11.5 and E12.5, probably due to a placental defect
• Background Sensitivity: the frequency of live homozygotes developing after E11.5 is markedly reduced upon backcrossing into the C57BL/6J background
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hematopoietic system
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• at E11.5 or later, live homozygotes exhibit a severe block of erythroid differentiation in fetal liver, indicating defective definitive erythropoiesis, whereas embryonic erythropoiesis remains unaffected
• failure of definitive erythropoiesis is caused by diminished erythropoietin gene expression
• however, mutant fetal livers contain BFU-Es and CFU-Es at normal frequencies, reconstitute erythropoiesis in lethally irradiated hosts, and undergo terminal differentiation in vitro in the presence of exogenous erythropoietin
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liver/biliary system
small liver
(
J:63425
)
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• at E11.5 or later, live homozygotes exhibit small fetal livers
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pale liver
(
J:63425
)
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• at E11.5 or later, live homozygotes exhibit pale fetal livers
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embryo
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• homozygotes that die at E11.5 display a sigificant reduction of embryo-derived blood vessels in the placental labyrinth layer
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• some of the homozygotes that die between E11.5 and E12.5 appear growth-retarded
• in contrast, homozygotes that survive beyond E12.5 exhibit normal growth and morphology
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pale yolk sac
(
J:63425
)
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• at E11.5 or later, live homozygotes display normal-sized yolk sacs with only a few circulating red blood cells
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growth/size/body
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• some of the homozygotes that die between E11.5 and E12.5 appear growth-retarded
• in contrast, homozygotes that survive beyond E12.5 exhibit normal growth and morphology
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integument
cardiovascular system
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• homozygotes that die at E11.5 display a sigificant reduction of embryo-derived blood vessels in the placental labyrinth layer
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