Phenotypes associated with this allele

• Allele Symbol: Mapk14^tm1Mms
• Allele Name: targeted mutation 1, Michael M Shen
• Allele ID: MGI:2177832
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mapk14^tm1Mms/Mapk14^tm1Mms	involves: 129S7/SvEvBrd * C57BL/6	MGI:3513555

Genotype
MGI:3513555

hm1

• Allelic Composition: Mapk14^tm1Mms/Mapk14^tm1Mms
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Morphology and histology of wild type and Mapk14^tm1Mms/Mapk14^tm1Mms mouse embryos and placentas

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:73417 )

• homozygotes die in utero between 10.5 and 12.5 dpc

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:73417 )

• at 10.5 dpc, the angiogenic mesenchyme fails to infiltrate the developing labyrinth layer

• by 11.5 dpc, homozygotes exhibit a severely impaired vascular network within the labyrinth, with a nearly complete loss of blood vessels lined with endothelial cells

abnormal angiogenesis ( J:73417 )

• at 10.5 dpc, homozygotes display an angiogenic defect in embryonic and extraembryonic tissues that correlates with impaired placental vascularization

• at 10.5 dpc, mutant brains fail to show angiogenic pruning and remodeling of the vascular plexus, displaying numerous vessels of similar size that are not organized in a branching vascular tree

cellular

abnormal cell physiology ( J:73417 )

• mutant ES cells show no overt growth or differentiation phenotypes in early embryoid body culture

• notably, attempts to culture primary MEFs are unsuccessful due to cellular inviability following the first passage

embryo

embryonic growth retardation ( J:73417 )

• by 11.5 dpc, mutant embryos are often developmentally retarded and smaller than wild-type embryos

abnormal placenta morphology ( J:73417 )

• at 10.5 dpc, the mutant placenta appears thinner and paler than normal; however, the chorioallantoic fusion appears unaffected

abnormal placental labyrinth vasculature morphology ( J:73417 )

• at 10.5 dpc, the angiogenic mesenchyme fails to infiltrate the developing labyrinth layer

• by 11.5 dpc, homozygotes exhibit a severely impaired vascular network within the labyrinth, with a nearly complete loss of blood vessels lined with endothelial cells

decreased trophoblast giant cell number ( J:73417 )

• at 10.5 dpc, homozygotes exhibit a highly abnormal diploid trophoblast with abundant apoptotic cells in the placental labyrinth layer; no differences are noted in the trophoblast giant cell population

decreased spongiotrophoblast size ( J:73417 )

• at 10.5 dpc, the mutant spongiotrophoblast layer is significantly reduced

absent placental labyrinth ( J:73417 )

• at 10.5 dpc, the developing labyrinth of homozygotes is significantly thinner than that of wild-type embryos

• by 11.5 dpc, the mutant labyrinth layer is essentially absent

abnormal vitelline vascular remodeling ( J:73417 )

• at 10.5 dpc, mutant visceral yolk sacs lack major blood vessels and retain a primitive capillary plexus

growth/size/body

embryonic growth retardation ( J:73417 )

• by 11.5 dpc, mutant embryos are often developmentally retarded and smaller than wild-type embryos

hematopoietic system

hematopoietic system phenotype ( J:73417 )

N • mutant embryos display no gross abnormalities in primitive hematopoiesis or cardiac development