Phenotypes associated with this allele

• Allele Symbol: Krt19^tm2Mmt
• Allele Name: targeted mutation 2, Makoto M Taketo
• Allele ID: MGI:2178046
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Krt19^tm2Mmt/Krt19^tm2Mmt	involves: 129S2/SvPas * C57BL/6	MGI:2672100
hm2	Krt19^tm2Mmt/Krt19^tm2Mmt	involves: 129S2/SvPas * C57BL/6 * FVB/N	MGI:3850372
cx3	Krt19^tm2Mmt/Krt19^tm2Mmt Krt8^tm1Rgo/Krt8^tm1Rgo	FVB.129S2-Krt19^tm2Mmt Krt8^tm1Rgo	MGI:3850373
cx4	Krt18^tm1Tmm/Krt18^tm1Tmm Krt19^tm2Mmt/Krt19^tm2Mmt	involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB/N	MGI:3582985

Genotype
MGI:2672100

hm1

• Allelic Composition: Krt19^tm2Mmt/Krt19^tm2Mmt
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:65498 )

• homozygotes are viable, fertile, and phenotypically indistinguishable from their wild-type and heterozygous control littermates

Genotype
MGI:3850372

hm2

• Allelic Composition: Krt19^tm2Mmt/Krt19^tm2Mmt
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * FVB/N

mortality/aging

preweaning lethality, incomplete penetrance ( J:65498 )

• Background Sensitivity: fewer homozygotes than expected are recovered after one or two backcrosses to FBV/N whereas normal numbers of homozygotes are obtained from heterozygous crosses between parents in the C57BL/6 and 129/Sv backgrounds (F1) or after one or two backcrosses to C57BL/6

• however, surviving homozygotes are viable, fertile, and phenotypically indistinguishable from heterozygous and wild-type littermates in terms of gross anatomy, histology, and behavior up to 20 months of age

Genotype
MGI:3850373

cx3

• Allelic Composition: Krt19^tm2Mmt/Krt19^tm2Mmt; Krt8^tm1Rgo/Krt8^tm1Rgo
• Genetic Background: FVB.129S2-Krt19^tm2Mmt Krt8^tm1Rgo

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:65498 )

• after E9.5, the number of double homozygotes declines gradually, with increasing numbers of necrotic remnants and resorption sites

• although some double homozygotes are still alive at E9.75-E10.5, they exhibit small placentas and varying degrees of growth retardation

embryo

embryonic growth retardation ( J:65498 )

• at E9.75-E10.5, live double homozygotes display varying degrees of growth retardation

abnormal allantois morphology ( J:65498 )

• at E10.5, the allantois is poorly formed and shows signs of degeneration

abnormal placenta morphology ( J:65498 )

• as early as E9.5, double homozygotes display placental defects that cause flooding of the maternal blood into the embryonic placenta

abnormal placenta labyrinth morphology ( J:65498 )

• at E9.5, labyrinthine trophoblast cells are decreased in number and appear to be poorly organized

• at E10.5, degeneration of the labyrinthine trophoblasts is observed

abnormal placental labyrinth vasculature morphology ( J:65498 )

• by E10.5, placental vascular structures are deteriorated while maternal and embryonic blood cells display signs of degeneration

small placenta ( J:65498 )

• at E9.75-E10.5, placentas are smaller than normal

abnormal trophoblast layer morphology ( J:65498 )

• at E10.5, trophoblastic tissues contain lesions filled with unnucleated maternal erythrocytes as well as some large and nucleated embryonic erythrocytes

abnormal trophoblast giant cell morphology ( J:65498 )

• at E9.5, giant trophoblasts are pulled apart and display significantly larger cell bodies and nuclei

increased trophoblast giant cell number ( J:65498 )

• a significant increase in the number of secondary giant cells is noted at E9.5

• at E10.5, multiple layers of giant cells are observed

abnormal spongiotrophoblast layer morphology ( J:65498 )

• at E10.5, degeneration of the spongiotrophoblasts is observed

abnormal spongiotrophoblast cell morphology ( J:65498 )

• at E9.5, spongiotrophoblast cells appear to be poorly organized

decreased spongiotrophoblast cell number ( J:65498 )

• at E9.5, spongiotrophoblast cells are decreased in number

impaired placental function ( J:65498 )

• at E9.5, double homozygotes display flooding of maternal blood into the embryonic placenta in the absence of overt clotting

growth/size/body

embryonic growth retardation ( J:65498 )

• at E9.75-E10.5, live double homozygotes display varying degrees of growth retardation

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:65498 )

• by E10.5, placental vascular structures are deteriorated while maternal and embryonic blood cells display signs of degeneration

Genotype
MGI:3582985

cx4

• Allelic Composition: Krt18^tm1Tmm/Krt18^tm1Tmm; Krt19^tm2Mmt/Krt19^tm2Mmt
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * 129S2/SvPas * C57BL/6 * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:65090 )

• no homozygotes at birth

• embryonic death between E9.5 and E10.5

embryo

decreased embryo size ( J:65090 )

• embryos smaller than controls but otherwise normal at E9.5

abnormal extraembryonic tissue morphology ( J:65090 )

• bleeding in extraembryonic tissues between the maternal deciduas and yolk sac at E10.5

abnormal trophoblast giant cell morphology ( J:65090 )

• trophoblast giant cells were cytolytic

small placenta ( J:65090 )

• placentas smaller in diameter but otherwise normally developed

growth/size/body

decreased embryo size ( J:65090 )

• embryos smaller than controls but otherwise normal at E9.5