Phenotypes associated with this allele

• Allele Symbol: Gria2^tm2Rsp
• Allele Name: targeted mutation 2, Rolf Sprengel
• Allele ID: MGI:2178121
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gria2^tm2Rsp/Gria2^tm2Rsp	involves: 129S1/Sv * 129X1/SvJ	MGI:3612255
ht2	Gria2^tm2Rsp/Gria2^+	involves: 129S1/Sv * 129X1/SvJ	MGI:3612382
cn3	Gria2^tm2Rsp/Gria2^+ Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3686594
cn4	Gria2^tm2Rsp/Gria2^+ Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * NMRI	MGI:3612397
cx5	Gria2^tm2Rsp/Gria2^+ Tg(Gria2*586N)238.2Rsp/0	involves: 129S1/Sv * 129X1/SvJ	MGI:3612464

Genotype
MGI:3612255

hm1

• Allelic Composition: Gria2^tm2Rsp/Gria2^tm2Rsp
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:51845 )

• die between P4 and P20

growth/size/body

decreased body weight ( J:51845 )

postnatal growth retardation ( J:51845 )

nervous system

abnormal hippocampus CA3 region morphology ( J:51845 )

• basal dendrites of CA3 cells are reduced in length and in number of branching points

abnormal excitatory postsynaptic potential ( J:51845 )

• require 30% higher stimulation currents to elicit fEPSPs of the same amplitude as wild-type and the number of failures to induce LTP is increased compared to wild-type

abnormal AMPA-mediated synaptic currents ( J:51845 )

• AMPA receptor currents desensitize faster and have a smaller steady-state component than wild-type

• 29-fold increase relative to wild-type in fractional calcium currents (P_f values) of AMPA receptors in CA1 pyramidal cells at a membrane potential of -60 mV, however never observe seizures

behavior/neurological

lethargy ( J:51845 )

• show stupor from P15 onward and are hypoexcitable

Genotype
MGI:3612382

ht2

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

premature death ( J:51845 )

• 20% lethality

nervous system

abnormal AMPA-mediated synaptic currents ( J:51845 )

• AMPA receptors have a 2-fold increase in calcium permeability in CA1 pyramidal cells, but the lack of current rectification is comparable to wild-type responses

Genotype
MGI:3686594

cn3

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

behavior/neurological

seizures ( J:111689 )

• seizure episodes are observed, while none are seen in forebrain-specific knockouts

nervous system

seizures ( J:111689 )

• seizure episodes are observed, while none are seen in forebrain-specific knockouts

abnormal neuron differentiation ( J:111689 )

• 2-fold increase in mitotic and postmitotic neurogenic cells is observed in subgranular zone (SGZ)

abnormal brain interneuron morphology ( J:111689 )

• there is loss of interneurons in hippocampus

loss of hippocampal neurons ( J:111689 )

• loss of somatostatin-positive interneurons in hilus and stratum oriens of the CA1 is observed

abnormal CNS glial cell morphology ( J:111689 )

• there are an increased number of glia seen in the amydala, motor cortex and somatosensory cortex

cellular

abnormal neuron differentiation ( J:111689 )

• 2-fold increase in mitotic and postmitotic neurogenic cells is observed in subgranular zone (SGZ)

Genotype
MGI:3612397

cn4

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N * NMRI

behavior/neurological

abnormal olfactory discrimination memory ( J:103689 )

• exhibit more rapid odor learning and enhanced olfactory discrimination in a go/no-go operant conditioning task compared to controls

Genotype
MGI:3612464

cx5

• Allelic Composition: Gria2^tm2Rsp/Gria2⁺; Tg(Gria2*586N)238.2Rsp/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

premature death ( J:51845 )

• die before P25

nervous system

seizures ( J:51845 )

• exibit periodic spontaneous epileptic seizures that start at P14/15

abnormal hippocampus CA3 region morphology ( J:51845 )

• develop neurosclerosis in CA3 subfield

abnormal AMPA-mediated synaptic currents ( J:51845 )

• calcium permeability of AMPA receptors in CA1 pyramidal neurons is increased about 4-fold relative to wild-type

• significant increase in the macroscopic AMPA receptor conductance in nucleated patches of CA1 pyramidal cells

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:51845 )

• mutants are hyperexcitable

seizures ( J:51845 )

• exibit periodic spontaneous epileptic seizures that start at P14/15