Phenotypes associated with this allele

• Allele Symbol: Mycn^tm1Fwa
• Allele Name: targeted mutation 1, Frederick W Alt
• Allele ID: MGI:2178216
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mycn^tm1Fwa/Mycn^tm1Fwa	involves: 129S/SvEv	MGI:4888961
hm2	Mycn^tm1Fwa/Mycn^tm1Fwa	involves: 129S/SvEv * MF1	MGI:2180011

Genotype
MGI:4888961

hm1

• Allelic Composition: Mycn^tm1Fwa/Mycn^tm1Fwa
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

increased hepatocyte apoptosis ( J:46520 )

• at E11.5, but not at E10.5, all mutant embryos show a 50-fold increase in hepatocyte apoptosis relative to wild-type controls

abnormal liver morphology ( J:46520 )

• at E11.5, mutant livers are smaller, consist of only one lobe, and show a 2-fold reduction in the number of hematopoietic cells

abnormal liver development ( J:46520 )

• failure of mutant livers to develop into a multi-lobular organ

small liver ( J:46520 )

• at E11.5, mutant livers are smaller than wild-type

liver hypoplasia ( J:46520 )

• at E11.5, mutant livers are less densely populated than wild-type

hematopoietic system

decreased hematopoietic stem cell number ( J:46520 )

• at E11.5, mutant livers show a 2-fold reduction in the number of hematopoietic cells relative to wild-type controls

cellular

increased hepatocyte apoptosis ( J:46520 )

• at E11.5, but not at E10.5, all mutant embryos show a 50-fold increase in hepatocyte apoptosis relative to wild-type controls

Genotype
MGI:2180011

hm2

• Allelic Composition: Mycn^tm1Fwa/Mycn^tm1Fwa
• Genetic Background: involves: 129S/SvEv * MF1

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:3413 )

• homozygotes die between E10.5 and E12.5, probably due to heart failure

cardiovascular system

abnormal anterior cardinal vein morphology ( J:3413 )

• at E11.5, two of 3 mutant embryos display an enlarged or dilated anterior cardinal vein

trabecula carnea hypoplasia ( J:3413 )

• at E10.5 and E11.5, some mutant embryos display reduced trabeculation of the ventricles

abnormal cardiac epithelial to mesenchymal transition ( J:3413 )

• at E10.5 and E11.5, the endocardium is still present in some embryos, indicating failure to undergo epithelial-mesenchymal transformation

abnormal heart development ( J:3413 )

• at E9.5, the ventricular region has not yet divided into chambers and trabeculation of the ventricle is reduced, unlike in wild-type hearts

• at E10.5, all mutant hearts appear significantly underdeveloped

• at E10.5, all mutant hearts retain the S-shape typical of E9.5 wild-type hearts; also seen in 1 of 3 mutant hearts at E11.5

• at E11.5, some mutant hearts contain atria and ventricular chambers but show underdeveloped valvular and septal tissues and trabecula carnea

embryo

decreased embryo size ( J:3413 )

• homozygous mutant embryos are often, but not always, reduced in size

• at E9.5-E11.5, three of 12 mutant embryos show severely reduced cellularity, as reflected by the amount of extractable RNA

abnormal mesonephros morphology ( J:3413 )

• at E10.5, the number of mesonephric tubules is reduced

abnormal embryonic neuroepithelium morphology ( J:3413 )

• at E11.5, some mutant embryos display irregularity and reduced cellularity in the neural ectoderm of the telencephalon and the myelencephalon

growth/size/body

decreased embryo size ( J:3413 )

• homozygous mutant embryos are often, but not always, reduced in size

• at E9.5-E11.5, three of 12 mutant embryos show severely reduced cellularity, as reflected by the amount of extractable RNA

nervous system

abnormal embryonic neuroepithelium morphology ( J:3413 )

• at E11.5, some mutant embryos display irregularity and reduced cellularity in the neural ectoderm of the telencephalon and the myelencephalon

abnormal cranial ganglia morphology ( J:3413 )

• at E10.5 and E11.5, mutant cranial ganglia, including the acoustico-facialis ganglia, are reduced in size and cellularity

small trigeminal ganglion ( J:3413 )

• at E10.5 and E11.5, mutant trigeminal ganglia are reduced in size and cellularity

small dorsal root ganglion ( J:3413 )

• at E10.5 and E11.5, mutant spinal ganglia are reduced in size

dorsal root ganglion hypoplasia ( J:3413 )

• at E10.5 and E11.5, mutant spinal ganglia show reduced cellularity

muscle

trabecula carnea hypoplasia ( J:3413 )

• at E10.5 and E11.5, some mutant embryos display reduced trabeculation of the ventricles

vision/eye

abnormal lens vesicle development ( J:3413 )

• invagination of the lens vesicles is slightly delayed but normal

abnormal optic vesicle formation ( J:3413 )

• invagination of the optic vesicles is slightly delayed but normal