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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Spo11tm1Mjn
targeted mutation 1, Maria Jasin
MGI:2178805
Summary 20 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Spo11tm1Mjn/Spo11tm1Mjn involves: 129X1/SvJ MGI:5427613
hm2
 		Spo11tm1Mjn/Spo11tm1Mjn involves: 129X1/SvJ * C57BL/6 MGI:4358251
hm3
 		Spo11tm1Mjn/Spo11tm1Mjn involves: 129X1/SvJ * C57BL/6J MGI:5438302
hm4
 		Spo11tm1Mjn/Spo11tm1Mjn involves: 129X1/SvJ * C57BL/6JOlaHsd MGI:5013565
ht5
 		Spo11tm1Mjn/Spo11+ B6.Cg-Spo11tm1Mjn MGI:6506424
cn6
 		Fignl1tm1Osb/Fignl1tm1.1Osb
Spo11tm1Mjn/Spo11tm1Mjn
Tg(Stra8-cre)1Osb/0 		involves: 129X1/SvJ * C57BL/6 MGI:7546968
cx7
 		Spo11tm1Mjn/Spo11+
Stag3tm1e.1(KOMP)Wtsi/Stag3tm1e.1(KOMP)Wtsi 		B6.Cg-Spo11tm1Mjn Stag3tm1e.1(KOMP)Wtsi/2J MGI:6506426
cx8
 		Hormad2tm1.2Atot/Hormad2tm1.2Atot
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 * SJL MGI:5427615
cx9
 		Hormad2tm1Atot/Hormad2tm1Atot
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6JOlaHsd MGI:5427614
cx10
 		Hormad1tm1Atot/Hormad1tm1Atot
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6JOlaHsd MGI:5013559
cx11
 		Rec8mei8/Rec8mei8
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129S4/SvJae * 129X1/SvJ * C57BL/6J MGI:5615728
cx12
 		Spo11tm1Mjn/Spo11tm1Mjn
Syce2Gt(FHCRC-GT-S8-7E1)Sor/Syce2Gt(FHCRC-GT-S8-7E1)Sor 		involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6JOlaHsd MGI:5013566
cx13
 		Hormad1tm1.2Atot/Hormad1tm1.2Atot
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129/Sv * BALB/c * C57BL/6 * SJL MGI:5013563
cx14
 		Rad50tm4.1Jpt/Rad50+
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129/Sv * C57BL/6 MGI:5614080
cx15
 		Mcmdc2tm3b(EUCOMM)Hmgu/Mcmdc2tm3b(EUCOMM)Hmgu
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * BALB/c * C57BL/6 * C57BL/6N * CD-1 MGI:5898318
cx16
 		Hormad2tm1Kura/Hormad2+
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj MGI:5466573
cx17
 		Hormad2tm1Kura/Hormad2tm1Kura
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj MGI:5466574
cx18
 		Rad21ltm1Yow/Rad21ltm1Yow
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj MGI:5615730
cx19
 		Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * C57BL/6N MGI:5897826
cx20
 		Hormad1tm1Kura/Hormad1tm1Kura
Spo11tm1Mjn/Spo11tm1Mjn 		involves: 129X1/SvJ * C57BL/6NCrlj * CBA/JNCrlj MGI:5587825


Genotype
MGI:5427613
hm1
Allelic
Composition		 Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased oocyte number ( J:183982 , J:192634 )
• much lower numbers at 6 weeks of age (J:183982)
(J:192634)
abnormal meiosis ( J:183982 )
• a subset of unsynapsed chromosome axes are surrounded by H2AFX rich chromatin domains forming pseudo-sex bodies
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired
abnormal synaptonemal complex ( J:236226 )
• extensive non-homologous synaptonemal complex formation

reproductive system
decreased oocyte number ( J:183982 , J:192634 )
• much lower numbers at 6 weeks of age (J:183982)
(J:192634)
abnormal meiosis ( J:183982 )
• a subset of unsynapsed chromosome axes are surrounded by H2AFX rich chromatin domains forming pseudo-sex bodies
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired
abnormal synaptonemal complex ( J:236226 )
• extensive non-homologous synaptonemal complex formation

endocrine/exocrine glands




Genotype
MGI:4358251
hm2
Allelic
Composition		 Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
azoospermia ( J:65880 )
• absence of epididymal sperm
abnormal male meiosis ( J:65880 )
• few or no cells progress through meiosis
• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• spermatocytes are seen with much longer axial elements and short synaptonemal complexes, with longer synaptonemal complexes but axial elements involved in synapsis with several partners, or without synaptonemal complexes
• spermatocyte nuclei with complete synapsis are not seen
abnormal oogenesis ( J:65880 )
• at E15.5 most oocytes appear to be in the leptotene or early zygotene stage
• at E16.5 most oocytes still have an early zygotene morphology with a few reaching an abnormal zygotene like stage with long axial elements in synapsis but with partner exchanges
• at E17.5 most nuclei are either in an abnormal zygotene like stage or have appear to be fully synapsed but lack Scp1 expression
decreased oocyte number ( J:65880 )
• normal numbers of oogonia are present at birth but oocyte numbers decline rapidly after birth
• at P4 and P8 only 10% - 20% of the normal number of c-kit positive oocytes are seen
• at 8 weeks of age oocyte depletion is more pronounced
abnormal female meiosis ( J:65880 )
• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• oocytes display defects in synapsis

reproductive system
abnormal oogenesis ( J:65880 )
• at E15.5 most oocytes appear to be in the leptotene or early zygotene stage
• at E16.5 most oocytes still have an early zygotene morphology with a few reaching an abnormal zygotene like stage with long axial elements in synapsis but with partner exchanges
• at E17.5 most nuclei are either in an abnormal zygotene like stage or have appear to be fully synapsed but lack Scp1 expression
decreased oocyte number ( J:65880 )
• normal numbers of oogonia are present at birth but oocyte numbers decline rapidly after birth
• at P4 and P8 only 10% - 20% of the normal number of c-kit positive oocytes are seen
• at 8 weeks of age oocyte depletion is more pronounced
abnormal female meiosis ( J:65880 )
• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• oocytes display defects in synapsis
abnormal ovary morphology ( J:65880 )
• at 8 weeks of age ovarian dysgenesis is apparent
decreased primordial ovarian follicle number ( J:65880 )
• at P13 the cortex of the ovary is nearly devoid of primordial follicles
impaired ovarian folliculogenesis ( J:65880 )
• at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold
• at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen
small ovary ( J:65880 )
• slightly smaller compared to wild-type mice
decreased testis weight ( J:65880 )
• at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates
abnormal spermatogenesis ( J:65880 )
• increase in the incidence of apoptotic cells in the tubules
azoospermia ( J:65880 )
• absence of epididymal sperm
abnormal male meiosis ( J:65880 )
• few or no cells progress through meiosis
• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• spermatocytes are seen with much longer axial elements and short synaptonemal complexes, with longer synaptonemal complexes but axial elements involved in synapsis with several partners, or without synaptonemal complexes
• spermatocyte nuclei with complete synapsis are not seen
arrest of spermatogenesis ( J:65880 )
• seminiferous tubules generally contain only spermatogonia and Sertoli cells or spermatogonia and Sertoli cells plus either early prophase spermatocytes or cells with highly condensed chromatin fragments
• no spermatocytes with pachytene or later morphology are seen

immune system
immune system phenotype ( J:74754 )
N
• despite expression patterns and the known role in double strand breaks in meiosis, no abnormalities in somatic hypermutation or class switch recombination are detected

endocrine/exocrine glands
abnormal ovary morphology ( J:65880 )
• at 8 weeks of age ovarian dysgenesis is apparent
decreased primordial ovarian follicle number ( J:65880 )
• at P13 the cortex of the ovary is nearly devoid of primordial follicles
impaired ovarian folliculogenesis ( J:65880 )
• at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold
• at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen
small ovary ( J:65880 )
• slightly smaller compared to wild-type mice
decreased testis weight ( J:65880 )
• at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates




Genotype
MGI:5438302
hm3
Allelic
Composition		 Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal chromosomal synapsis ( J:209347 )
• spermatocytes exhibit homolog association and pairing at all tested chromosomal regions, although less than in wild-type spermatocytes
abnormal male meiosis ( J:187173 , J:209347 )
• spermocytes exhibit neither early centromere-associations nor preferential initiation of synaptonemal complex formation from centromeres (J:187173)
• spermatocytes form cohesion axes and axial elements but fail to undergo proper synapsis, arresting at the late leptotene or zygotene-like stage (J:209347)
• spermatocytes exhibit homolog association and pairing at all tested chromosomal regions, although less than in wild-type spermatocytes (J:209347)
arrest of male meiosis ( J:209347 )
• spermatocytes arrest at the late leptotene or zygotene-like stage

cellular
abnormal chromosomal synapsis ( J:209347 )
• spermatocytes exhibit homolog association and pairing at all tested chromosomal regions, although less than in wild-type spermatocytes
abnormal male meiosis ( J:187173 , J:209347 )
• spermocytes exhibit neither early centromere-associations nor preferential initiation of synaptonemal complex formation from centromeres (J:187173)
• spermatocytes form cohesion axes and axial elements but fail to undergo proper synapsis, arresting at the late leptotene or zygotene-like stage (J:209347)
• spermatocytes exhibit homolog association and pairing at all tested chromosomal regions, although less than in wild-type spermatocytes (J:209347)
arrest of male meiosis ( J:209347 )
• spermatocytes arrest at the late leptotene or zygotene-like stage
abnormal double-strand DNA break repair ( J:209347 )
• double-strand break formation is abolished

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:209347 )
• double-strand break formation is abolished




Genotype
MGI:5013565
hm4
Allelic
Composition		 Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6JOlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased oocyte number ( J:173089 )
• very few oocytes survive until 6 weeks unlike in wild-type mice
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
increased female germ cell apoptosis ( J:173089 )
• at P1, oocyte apoptosis rates are higher than in wild-type cells

reproductive system
decreased oocyte number ( J:173089 )
• very few oocytes survive until 6 weeks unlike in wild-type mice
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
increased female germ cell apoptosis ( J:173089 )
• at P1, oocyte apoptosis rates are higher than in wild-type cells
arrest of spermatogenesis ( J:173089 )
• spermatocytes are eliminated in stage-IV tubules unlike in wild-type mice




Genotype
MGI:6506424
ht5
Allelic
Composition		 Spo11tm1Mjn/Spo11+
Genetic
Background		 B6.Cg-Spo11tm1Mjn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (67% of WT) in zygotene spermatocytes

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (67% of WT) in zygotene spermatocytes




Genotype
MGI:7546968
cn6
Allelic
Composition		 Fignl1tm1Osb/Fignl1tm1.1Osb
Spo11tm1Mjn/Spo11tm1Mjn
Tg(Stra8-cre)1Osb/0
Genetic
Background		 involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fignl1tm1.1Osb mutation (0 available); any Fignl1 mutation (61 available)
Fignl1tm1Osb mutation (0 available); any Fignl1 mutation (61 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
Tg(Stra8-cre)1Osb mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
arrest of male meiosis ( J:342157 )
• cells are fully lost before pachynema

cellular
arrest of male meiosis ( J:342157 )
• cells are fully lost before pachynema




Genotype
MGI:6506426
cx7
Allelic
Composition		 Spo11tm1Mjn/Spo11+
Stag3tm1e.1(KOMP)Wtsi/Stag3tm1e.1(KOMP)Wtsi
Genetic
Background		 B6.Cg-Spo11tm1Mjn Stag3tm1e.1(KOMP)Wtsi/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
Stag3tm1e.1(KOMP)Wtsi mutation (1 available); any Stag3 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal double-strand DNA break repair ( J:280261 )
• complete absence of double-strand DNA breaks in 77% of zygotene spermatocytes and 50% reduction (compared to either single KO) in remaining 23% of spermatocytes

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:280261 )
• complete absence of double-strand DNA breaks in 77% of zygotene spermatocytes and 50% reduction (compared to either single KO) in remaining 23% of spermatocytes




Genotype
MGI:5427615
cx8
Allelic
Composition		 Hormad2tm1.2Atot/Hormad2tm1.2Atot
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad2tm1.2Atot mutation (0 available); any Hormad2 mutation (33 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:183982 )
N
• oocyte numbers are similar to wild-type females
abnormal meiosis ( J:183982 )
• significant decrease in the frequency of pseudo-sex bodies compared to mutant mice wild-type for Hormad2

cellular
abnormal meiosis ( J:183982 )
• significant decrease in the frequency of pseudo-sex bodies compared to mutant mice wild-type for Hormad2




Genotype
MGI:5427614
cx9
Allelic
Composition		 Hormad2tm1Atot/Hormad2tm1Atot
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6JOlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad2tm1Atot mutation (0 available); any Hormad2 mutation (33 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:183982 )
N
• oocyte numbers are similar to wild-type females
abnormal meiosis ( J:183982 )
• significant decrease in the frequency of pseudo-sex bodies compared to mutant mice wild-type for Hormad2

cellular
abnormal meiosis ( J:183982 )
• significant decrease in the frequency of pseudo-sex bodies compared to mutant mice wild-type for Hormad2




Genotype
MGI:5013559
cx10
Allelic
Composition		 Hormad1tm1Atot/Hormad1tm1Atot
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6JOlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad1tm1Atot mutation (0 available); any Hormad1 mutation (32 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:173089 )
N
• unlike in Spo11tm1Sky homozygotes, oocyte numbers are normal
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
abnormal male meiosis ( J:173089 )
• sex-body formation is impaired compared to in wild-type cells
abnormal synaptonemal complex ( J:173089 )
• synaptonemal-complex defects are more severe than either single homozygote

cellular
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
abnormal male meiosis ( J:173089 )
• sex-body formation is impaired compared to in wild-type cells
abnormal synaptonemal complex ( J:173089 )
• synaptonemal-complex defects are more severe than either single homozygote




Genotype
MGI:5615728
cx11
Allelic
Composition		 Rec8mei8/Rec8mei8
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129S4/SvJae * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rec8mei8 mutation (1 available); any Rec8 mutation (25 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal male meiosis ( J:209347 )
• spermatocytes exhibit axial element splitting in the zygotene-like stage
abnormal synaptonemal complex ( J:209347 )
• synaptonemal complex formation is largely abolished in spermatocytes

cellular
abnormal male meiosis ( J:209347 )
• spermatocytes exhibit axial element splitting in the zygotene-like stage
abnormal synaptonemal complex ( J:209347 )
• synaptonemal complex formation is largely abolished in spermatocytes




Genotype
MGI:5013566
cx12
Allelic
Composition		 Spo11tm1Mjn/Spo11tm1Mjn
Syce2Gt(FHCRC-GT-S8-7E1)Sor/Syce2Gt(FHCRC-GT-S8-7E1)Sor
Genetic
Background		 involves: 129S4/SvJaeSor * 129X1/SvJ * C57BL/6JOlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
Syce2Gt(FHCRC-GT-S8-7E1)Sor mutation (2 available); any Syce2 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
arrest of spermatogenesis ( J:173089 )
• spermatocytes are eliminated in stage-IV tubules unlike in wild-type mice




Genotype
MGI:5013563
cx13
Allelic
Composition		 Hormad1tm1.2Atot/Hormad1tm1.2Atot
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129/Sv * BALB/c * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad1tm1.2Atot mutation (0 available); any Hormad1 mutation (32 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:173089 )
N
• unlike in Spo11tm1Sky homozygotes, oocyte numbers are normal
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
abnormal male meiosis ( J:173089 , J:292036 )
• sex-body formation is impaired compared to in wild-type cells (J:173089)
• absence of double-stranded breaks (DSBs) in spermatocytes (J:292036)
• absence of BRME1 and RPA2 punctuate foci in spermatocytes (J:292036)
abnormal synaptonemal complex ( J:173089 )
• synaptonemal-complex defects are more severe than either single homozygote

cellular
abnormal female meiosis ( J:173089 )
• pseudo-sex-body-like formation is impaired compared to in wild-type cells
abnormal male meiosis ( J:173089 , J:292036 )
• sex-body formation is impaired compared to in wild-type cells (J:173089)
• absence of double-stranded breaks (DSBs) in spermatocytes (J:292036)
• absence of BRME1 and RPA2 punctuate foci in spermatocytes (J:292036)
abnormal synaptonemal complex ( J:173089 )
• synaptonemal-complex defects are more severe than either single homozygote




Genotype
MGI:5614080
cx14
Allelic
Composition		 Rad50tm4.1Jpt/Rad50+
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad50tm4.1Jpt mutation (0 available); any Rad50 mutation (53 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal seminiferous tubule morphology ( J:209141 )
• severely reduced cellularity

endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:209141 )
• severely reduced cellularity




Genotype
MGI:5898318
cx15
Allelic
Composition		 Mcmdc2tm3b(EUCOMM)Hmgu/Mcmdc2tm3b(EUCOMM)Hmgu
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * BALB/c * C57BL/6 * C57BL/6N * CD-1
Cell Lines HEPD0800_2_F07
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mcmdc2tm3b(EUCOMM)Hmgu mutation (0 available); any Mcmdc2 mutation (32 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal synaptonemal complex ( J:236226 )
• significantly more non-homologous synaptonemal complex stretches in spermatocytes than in Mcmdc2 single knockout but similar to Spo11 single knockout
abnormal double-strand DNA break repair ( J:236226 )
• localized H2AX-rich chromatin domains (pseudo-sex bodies)

reproductive system
abnormal synaptonemal complex ( J:236226 )
• significantly more non-homologous synaptonemal complex stretches in spermatocytes than in Mcmdc2 single knockout but similar to Spo11 single knockout

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:236226 )
• localized H2AX-rich chromatin domains (pseudo-sex bodies)




Genotype
MGI:5466573
cx16
Allelic
Composition		 Hormad2tm1Kura/Hormad2+
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad2tm1Kura mutation (1 available); any Hormad2 mutation (33 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:192634 )
N
• unlike Spo11tm1Mjn homozygotes, ovarian weights are normal
decreased oocyte number ( J:192634 )
• not as severe as in Spo11tm1Mjn homozygotes
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired

cellular
decreased oocyte number ( J:192634 )
• not as severe as in Spo11tm1Mjn homozygotes
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired




Genotype
MGI:5466574
cx17
Allelic
Composition		 Hormad2tm1Kura/Hormad2tm1Kura
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad2tm1Kura mutation (1 available); any Hormad2 mutation (33 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:192634 )
N
• unlike Spo11tm1Mjn homozygotes, mice exhibit normal ovarian weights and oocyte numbers
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired
abnormal testis morphology ( J:192634 )
• as in single homozytoes

endocrine/exocrine glands
abnormal testis morphology ( J:192634 )
• as in single homozytoes

cellular
abnormal female meiosis ( J:192634 )
• pseudo-sex body formation in oocytes is impaired




Genotype
MGI:5615730
cx18
Allelic
Composition		 Rad21ltm1Yow/Rad21ltm1Yow
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad21ltm1Yow mutation (1 available); any Rad21l mutation (34 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal chromosomal synapsis ( J:209347 )
• spermatocytes do not show significant homolog association
abnormal male meiosis ( J:209347 )
• spermatocytes exhibit bouquet arrest
abnormal synaptonemal complex ( J:209347 )
• synaptonemal complex formation is largely abolished in spermatocytes

cellular
abnormal chromosomal synapsis ( J:209347 )
• spermatocytes do not show significant homolog association
abnormal male meiosis ( J:209347 )
• spermatocytes exhibit bouquet arrest
abnormal synaptonemal complex ( J:209347 )
• synaptonemal complex formation is largely abolished in spermatocytes




Genotype
MGI:5897826
cx19
Allelic
Composition		 Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Meioctm1a(KOMP)Wtsi mutation (0 available); any Meioc mutation (38 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
arrest of male meiosis ( J:231231 )
• in early meiosis prophase I

cellular
arrest of male meiosis ( J:231231 )
• in early meiosis prophase I




Genotype
MGI:5587825
cx20
Allelic
Composition		 Hormad1tm1Kura/Hormad1tm1Kura
Spo11tm1Mjn/Spo11tm1Mjn
Genetic
Background		 involves: 129X1/SvJ * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hormad1tm1Kura mutation (1 available); any Hormad1 mutation (32 available)
Spo11tm1Mjn mutation (2 available); any Spo11 mutation (21 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:213984 )
N
• oocyte cell death observed in Spo11 null mice is abrogated
abnormal female meiosis ( J:213984 )
• oocytes lack pseudo sex bodies
abnormal male meiosis ( J:213984 )
• spermatocytes from stage IV seminiferous tubules is reduced
• however, spermatocytes from stages I to III seminiferous tubules form pseudo sex bodies
abnormal male germ cell apoptosis ( J:213984 )
• apoptotic cells at the pachytene stage

cellular
abnormal female meiosis ( J:213984 )
• oocytes lack pseudo sex bodies
abnormal male meiosis ( J:213984 )
• spermatocytes from stage IV seminiferous tubules is reduced
• however, spermatocytes from stages I to III seminiferous tubules form pseudo sex bodies
abnormal male germ cell apoptosis ( J:213984 )
• apoptotic cells at the pachytene stage




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory