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Allele Symbol Allele Name Allele ID |
Cer1tm1Bhr targeted mutation 1, Richard R Behringer MGI:2178818 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygous adults exhibit no obvious phenotypic defects and head formation, heart development, and somite formation are normal in embryos, however in embryonic tissue layer recombination assays, presomitic/somitic mesoderm is unable to maintain expression of the anterior neural marker Otx2 in ectoderm explants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• all triple mutant embryos at 7.5 dpc show morphological defects similar to or less severe than Cer1 Lefty1 double mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• by 9.5 dpc, 9.4% of embryos recovered are double nulls instead of expected 25%; surviving embryos are divided into classes I, II and III based on phenotypic characteristics
• Mendelian numbers are detected at 8.5 dpc
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• at 6.5 dpc, abnormalities can be observed, such as an accumulation of visceral endoderm cells at the presumptive anterior pole of the embryo
• abnormal thickening of the proximal anterior epiblast is observed and perisists to 7.5 dpc
• in some class II and III embryos, anterior and/or lateral protrusions ectoderm protrusions contact the opposite side of the ectoderm, leading to pinching of the embryonic region separating it into two or three distinct embryonic axes at 8.5 and 9.5 dpc
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• class I and II mutants have severely reduced amounts of lateral mesoderm cells
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• in class I and II mutants, paraxial mesoderm precursors are absent or misspecified
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• class III double mutants develop multiple primitive streaks at 8.5 and 9.5 dpc; ectopic primitive streak initiation begins later than the endogenous streak
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• expansion of the anterior primitive streak and its derivatives is observed in class I and II embryos at 7.5 dpc; anterior definitive endoderm is expanded in class I mutant embryos
• at 7.5 dpc in class II mutants, visceral endoderm cells persist in the anterior embryonic region
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• somites are not observed in some class III mutants at 8.5 and 9.5 dpc
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• class II and III double null embryos (29% of total) show separation of embryonic and extraembryonic regions by a tight constriction, resulting in physical separation of embryonic and extraembryonic ectoderm
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• in some double null embryos, accumulation of pyknotic cells arising from the ectodermal protrusions is observed in the amniotic cavity
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|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• all triple mutant embryos at 7.5 dpc show morphological defects similar to or less severe than Cer1 Lefty1 double mutants
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• the expected frequency of mutants are obtain and they appear phenotypically normal
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• by 9.5 dpc, 9.4% of embryos recovered are double nulls instead of expected 25%; surviving embryos are divided into classes I, II and III based on phenotypic characteristics
• Mendelian numbers are detected at 8.5 dpc
|
|
• at 6.5 dpc, abnormalities can be observed, such as an accumulation of visceral endoderm cells at the presumptive anterior pole of the embryo
• abnormal thickening of the proximal anterior epiblast is observed and perisists to 7.5 dpc
• in some class II and III embryos, anterior and/or lateral protrusions ectoderm protrusions contact the opposite side of the ectoderm, leading to pinching of the embryonic region separating it into two or three distinct embryonic axes at 8.5 and 9.5 dpc
• in some double null embryos, accumulation of pyknotic arising from the ectodermal protrusions is observed in the amniotic cavity
|
|
• class I and II mutants have severely reduced amounts of lateral mesoderm cells
|
|
• in class I and II mutants, paraxial mesoderm precursors are absent or misspecified
|
|
• class III double mutants develop multiple primitive streaks at 8.5 and 9.5 dpc; ectopic primitive streak initiation begins later than the endogenous streak
|
|
• expansion of the anterior primitive streak and its derivatives is observed in class I and II embryos at 7.5 dpc; anterior definitive endoderm is expanded in class I mutant embryos
• at 7.5 dpc in class II mutants, visceral endoderm cells persist in the anterior embryonic region
|
|
• somites are not observed in some class III mutants at 8.5 and 9.5 dpc
|
|
• class II and III double null embryos (29% of total) show separation of embryonic and extraembryonic regions by a tight constriction, resulting in physical separation of embryonic and extraembryonic ectoderm
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 07/05/2024 MGI 6.24 |
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