Analysis Tools
mortality/aging
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• no homozygous mutant embryos are recovered after E10.5
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embryo
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• at E7.0, wild-type embryos consist of three germ layers and are developing the posterior amniotic fold, whereas mutant embryos remain as bi-layered structures with one cavity resembling a pre-gastrula
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• at E7.0, the mutant ectoderm is thick, except for a small region immediately adjacent to the ectoplacental cone composed of a thinner layer of cells that most likely is extra-embryonic ectoderm
• by E8.5, the ectoderm and visceral endoderm continue to proliferate and excess ectoderm causes folding of the mutant embryos; extra-embryonic ectoderm is reduced
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• homozygotes fail to gastrulate
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• homozygotes lack anterior-posterior neural patterning
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• at E7.5, homozygous mutant embryos are of variable size
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• at E8.5, the mutant ectoderm fails to differentiate beyond the epiblast state, suggesting impaired epiblast patterning
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• at E6.5-E8.5, homozygotes consist of only ectoderm and visceral endoderm; no mesoderm is formed
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• at E7.5, homozygotes appear to lack nodal tissue
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• at E7.0, homozygotes lack an identifiable primitive streak
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• at E7.5, homozygotes lack distinct embryonic structures
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• at E8.5, homozygotes lack an identifiable allantois
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absent amnion
(
J:56511
)
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• at E8.5, homozygotes lack an identifiable amnion
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• at E7.0, homozygous mutant embryos fail to develop the posterior amniotic fold
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• at E8.5, homozygotes lack an identifiable chorion
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• at E8.5, homozygotes lack an identifiable yolk sac
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• at E6.5 and E7.5, initial development of the anterior visceral endoderm (AVE) appears to occur normally, but the overlying ectoderm does not acquire anterior neural identity
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growth/size/body
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• at E7.5, homozygous mutant embryos are of variable size
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limbs/digits/tail
absent limbs
(
J:56511
)
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• homozygotes never develop limbs
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