All Phenotypes Itk<tm1Ljb> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itk^tm1Ljb/Itk^tm1Ljb	B10.129-Itk^tm1Ljb	MGI:4354588
hm2	Itk^tm1Ljb/Itk^tm1Ljb	B6.129-Itk^tm1Ljb	MGI:4354503
hm3	Itk^tm1Ljb/Itk^tm1Ljb	involves: 129 * C57BL/6	MGI:4354505
cx4	Itk^tm1Ljb/Itk^tm1Ljb Txk^tm1Pls/Txk^tm1Pls	B6.129-Txk^tm1Pls Itk^tm1Ljb	MGI:4354591
cx5	Itk^tm1Ljb/Itk^tm1Ljb Rag1^tm1Mom/Rag1^tm1Mom Tg(Tcra5CC7,Tcrb5CC7)IWep/?	involves: 129 * C57BL/10	MGI:4354514
cx6	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra5CC7,Tcrb5CC7)IWep/?	involves: 129 * C57BL/10	MGI:4354593
cx7	Itk^tm1Ljb/Itk^tm1Ljb Tg(TcraTcrb)1100Mjb/?	involves: 129 * C57BL/6 * C57BL/10	MGI:4354589
cx8	Il15^tm1Imx/Il15^tm1Imx Itk^tm1Ljb/Itk^tm1Ljb	involves: 129 * C57BL/6 * C57BL/10	MGI:4354590
cx9	Itk^tm1Ljb/Itk^tm1Ljb Tcrd^tm1Mom/Tcrd^tm1Mom	involves: 129 * C57BL/6 * C57BL/10	MGI:4354603
cx10	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354594
cx11	H2^b/H2^b Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354597
cx12	H2^b/H2^k Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?	involves: C3H/HeJ * C57BL/6 * C57BL/10	MGI:4354598

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:113408 )

• CD8⁺CD44^hi thymocytes first appear at 4 days after birth

• these CD44^hiCD8⁺ thymocytes accumulate with age

• increased numbers of CD8⁺ SP thymocytes is accompanied by a transient deficit in the numbers of peripheral CD8⁺ T cells

increased germinal center B cell number ( J:148531 )

• germinal center B cells are increased almost two-fold compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• percentage of CD4 single positive thymoyctes is slightly decreased compared to controls

• the percentage of CD4⁺ T cells in the periphery is half that of controls

increased CD8-positive, alpha-beta T cell number ( J:113408 , J:123795 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus (J:113408)

• percentage of CD8 single positive thymocyotes are increased about 2-fold compared to controls (J:123795)

• these thymocytes have a memory phenotype based on CD44 expression (J:123795)

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal gamma-delta T cell morphology ( J:148531 )

• gamma-delta T cells have increased expression of CD4, NK1.1, CD44

• gamma-delta T cells also have increased expression ICOS that can promote B cell differentiation

• after activation, gamma-delta T cells increase expression of CD40L and OX40 that also promote B cell differentation

increased mature gamma-delta T cell number ( J:148531 )

• gamma delta T cell numbers are increased in the thymus, spleen, lymph nodes, and liver but not in the intestinal epithelium

• increases range 2 to 3 fold with a disproportionate increase in the Vgamma1.1 subset

increased IgE level ( J:148531 )

• IgE levels are highly elevated at 10 micrograms per ml compared to virtually none in controls

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

decreased interferon-gamma secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells have decreased IFN-gamma secretion after activation compared to controls

increased interferon-gamma secretion ( J:113408 )

• CD8⁺ T cells have increased secretion of IFN-gamma upon ex vivo stimulation by PMA and ionomycin

increased interleukin-10 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-10 on a per cell basis than controls after activation

increased interleukin-13 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-13 on a per cell basis than controls after activation

increased interleukin-4 secretion ( J:148531 )

• NK1.1⁺ gamma delta T cells secrete more IL-4 on a per cell basis than controls after activation

• CD4⁺ gamma delta T cells also produce more IL-4 than their wild-type counterparts

hematopoietic system

abnormal CD8-positive, alpha-beta T cell differentiation ( J:113408 )

• CD8⁺CD44^hi thymocytes first appear at 4 days after birth

• these CD44^hiCD8⁺ thymocytes accumulate with age

• increased numbers of CD8⁺ SP thymocytes is accompanied by a transient deficit in the numbers of peripheral CD8⁺ T cells

increased germinal center B cell number ( J:148531 )

• germinal center B cells are increased almost two-fold compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• percentage of CD4 single positive thymoyctes is slightly decreased compared to controls

• the percentage of CD4⁺ T cells in the periphery is half that of controls

increased CD8-positive, alpha-beta T cell number ( J:113408 , J:123795 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus (J:113408)

• percentage of CD8 single positive thymocyotes are increased about 2-fold compared to controls (J:123795)

• these thymocytes have a memory phenotype based on CD44 expression (J:123795)

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal gamma-delta T cell morphology ( J:148531 )

• gamma-delta T cells have increased expression of CD4, NK1.1, CD44

• gamma-delta T cells also have increased expression ICOS that can promote B cell differentiation

• after activation, gamma-delta T cells increase expression of CD40L and OX40 that also promote B cell differentation

increased mature gamma-delta T cell number ( J:148531 )

• gamma delta T cell numbers are increased in the thymus, spleen, lymph nodes, and liver but not in the intestinal epithelium

• increases range 2 to 3 fold with a disproportionate increase in the Vgamma1.1 subset

increased IgE level ( J:148531 )

• IgE levels are highly elevated at 10 micrograms per ml compared to virtually none in controls

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	B6.129-Itk^tm1Ljb

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal NK T cell morphology ( J:120689 )

• there is a partial block in NK T cell development in the thymus

• mice have twice the percentage of fraction 2 (Dex5⁺, NK1.1^-) cells as controls, with a concomitant reduction in fraction 3 Dex5⁺, NK1.1⁺cells

• the percentage of CD4⁺CD8⁺ double positive NK precursors is about 10-fold less than controls in the thymus

decreased NK T cell number ( J:120689 )

• NK T cells found in the spleen of 6-8 week old mice is about half that of controls

• by 20 weeks of age, NK T cell numbers have dropped to about a third of controls

• thymic numbers of mature NK T cells is 2-fold above normal at 6-8 weeks of age but then drops to a third of normal by 20 weeks of age

hematopoietic system

abnormal NK T cell morphology ( J:120689 )

• there is a partial block in NK T cell development in the thymus

• mice have twice the percentage of fraction 2 (Dex5⁺, NK1.1^-) cells as controls, with a concomitant reduction in fraction 3 Dex5⁺, NK1.1⁺cells

• the percentage of CD4⁺CD8⁺ double positive NK precursors is about 10-fold less than controls in the thymus

decreased NK T cell number ( J:120689 )

• NK T cells found in the spleen of 6-8 week old mice is about half that of controls

• by 20 weeks of age, NK T cell numbers have dropped to about a third of controls

• thymic numbers of mature NK T cells is 2-fold above normal at 6-8 weeks of age but then drops to a third of normal by 20 weeks of age

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:48231 )

• CD4⁺ T cell numbers are about half that of controls

abnormal T cell activation ( J:48231 )

• CD4⁺ T cells have less increases in intracellular calcium after high doses of anti-CD3 than controls

• at sub-optimal doses of anti-CD3, CD4⁺ T cells fail to flux any calcium

• calcium influx is to due defects in opening of plasma membrane channels and not from defects in internal calcium stores

decreased interleukin-2 secretion ( J:48231 )

• CD4⁺ T cell produce only half the amount of IL-2 as controls when stimulated with anti-CD3 and anti-CD28

• when PMA and ionomycin are used to activate cells IL-2 production is comparable to controls

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:48231 )

• CD4⁺ T cell numbers are about half that of controls

abnormal T cell activation ( J:48231 )

• CD4⁺ T cells have less increases in intracellular calcium after high doses of anti-CD3 than controls

• at sub-optimal doses of anti-CD3, CD4⁺ T cells fail to flux any calcium

• calcium influx is to due defects in opening of plasma membrane channels and not from defects in internal calcium stores

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Txk^tm1Pls/Txk^tm1Pls
Genetic Background	B6.129-Txk^tm1Pls Itk^tm1Ljb

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Txk^tm1Pls mutation (0 available); any Txk mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased CD8-positive, alpha-beta T cell number ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus

increased interferon-gamma secretion ( J:113408 )

• CD8⁺ T cells have increased secretion of IFN-gamma upon ex vivo stimulation by PMA and ionomycin

hematopoietic system

increased CD8-positive, alpha-beta T cell number ( J:113408 )

• vast majority of T cells have a memory cell phenotype including the ability to produce IFN-gamma upon ex vivo stimulation and upregulation of Bcl-xL in response to IL-15

increased memory T cell number ( J:113408 )

• majority of single-positive CD8⁺ T cell in the thymus have morphological characteristics similar to memory T cells (CD25^lo, CD44^hi, CD69^lo, CD122⁺, HSA^lo and NK1.1^int)

• 85% of peripheral CD8⁺ T cells are memory T cells based on surface markers

• memory T cell phenotype is confirmed functionally in that a large proportion of T cells produce IFN-gamma upon ex vivo stimulation and that they upregulate Bcl-xL in response to IL-15

abnormal CD8-positive, alpha-beta T cell physiology ( J:113408 )

• single-positive CD8⁺ T cell numbers are increased 3-fold in the thymus

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Rag1^tm1Mom/Rag1^tm1Mom Tg(Tcra5CC7,Tcrb5CC7)IWep/?
Genetic Background	involves: 129 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Rag1^tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tg(Tcra5CC7,Tcrb5CC7)IWep mutation (5 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal CD4-positive T cell differentiation ( J:93606 )

• when stimulated with low concentrations of antigen, CD4 T cells differentiate predominately into a Th1 phenotype while controls develop into a Th2 phenotype

• these T cells have increased expression of the T-bet transcription factor

decreased interferon-gamma secretion ( J:93606 )

• T cells cultured under Th1 polarizing conditions produce less IFN-gamma than controls

• Th1 T cells produce 1.5- to 200-fold less IFN-gamma than controls

decreased interleukin-10 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-10 than controls

decreased interleukin-4 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-4 than controls

• Th2 T cells produce 3.5- to 300-fold less IL-4 than controls

decreased interleukin-5 secretion ( J:93606 )

• T cells cultured under Th2 polarizing conditions produce less IL-5 than controls

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:93606 )

• when stimulated with low concentrations of antigen, CD4 T cells differentiate predominately into a Th1 phenotype while controls develop into a Th2 phenotype

• these T cells have increased expression of the T-bet transcription factor

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra5CC7,Tcrb5CC7)IWep/?
Genetic Background	involves: 129 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(Tcra5CC7,Tcrb5CC7)IWep mutation (5 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(TcraTcrb)1100Mjb/?
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:113408 )

N	• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in Itk^tm1Ljb homozygotes does not occur in these mice

Allelic Composition	Il15^tm1Imx/Il15^tm1Imx Itk^tm1Ljb/Itk^tm1Ljb
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il15^tm1Imx mutation (5 available); any Il15 mutation (44 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased CD8-positive, alpha-beta T cell number ( J:113408 )

• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in homozygote Itk^tm1Ljb thymus does not occur in these mice

• there is a 23-fold reduction in peripheral CD8⁺ T cells compared to wild-type mice and a 7-fold reduction compared to IL-15-sufficient Itk^tm1Ljb homozygotes

immune system

decreased CD8-positive, alpha-beta T cell number ( J:113408 )

• the altered CD8⁺ T cell development that generates a large proportion of memory T cells in homozygote Itk^tm1Ljb thymus does not occur in these mice

• there is a 23-fold reduction in peripheral CD8⁺ T cells compared to wild-type mice and a 7-fold reduction compared to IL-15-sufficient Itk^tm1Ljb homozygotes

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tcrd^tm1Mom/Tcrd^tm1Mom
Genetic Background	involves: 129 * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tcrd^tm1Mom mutation (13 available); any Tcrd mutation (16 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:148531 )

N	• the hyper-IgE and increased numbers of germinal center B cells are at normal levels in these mice

Allelic Composition	Itk^tm1Ljb/Itk^tm1Ljb Tg(Tcra2B4)1Mmd/? Tg(Tcrb2B4)1Mmd/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(Tcra2B4)1Mmd mutation (0 available)
Tg(Tcrb2B4)1Mmd mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the percentages and absolute numbers of CD4⁺ single positive thymocytes is drastically reduced compared to wild-type and transgenic controls

• peripheral CD4 T cell numbers are also reduced

Allelic Composition	H2^b/H2^b Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2^b mutation (30 available); any H2 mutation (282 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(TcrAND)53Hed mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• there are almost no mature transgenic CD4 T cells in the periphery

Allelic Composition	H2^b/H2^k Itk^tm1Ljb/Itk^tm1Ljb Tg(TcrAND)53Hed/?
Genetic Background	involves: C3H/HeJ * C57BL/6 * C57BL/10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2^b mutation (30 available); any H2 mutation (282 available)
H2^k mutation (14 available); any H2 mutation (282 available)
Itk^tm1Ljb mutation (0 available); any Itk mutation (50 available)
Tg(TcrAND)53Hed mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

hematopoietic system

abnormal positive T cell selection ( J:123795 )

• thymocytes take longer to undergo positive selection than wild-type thymocytes based on expression of CD69 and HSA

• expression of CD5, which is correlated with TCR signal strength, is also decreased

decreased CD4-positive, alpha-beta T cell number ( J:123795 )

• the development of transgenic CD4 T cells is reduced 2- to 3-fold in these mice

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/17/2024 MGI 6.24