Phenotypes associated with this allele

• Allele Symbol: Ddit3^tm1Aki
• Allele Name: targeted mutation 1, Shizuo Akira
• Allele ID: MGI:2179046
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ddit3^tm1Aki/Ddit3^tm1Aki	involves: 129P2/OlaHsd	MGI:3629963
hm2	Ddit3^tm1Aki/Ddit3^tm1Aki	involves: 129P2/OlaHsd * C57BL/6	MGI:3629964

Genotype
MGI:3629963

hm1

• Allelic Composition: Ddit3^tm1Aki/Ddit3^tm1Aki
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased neuron apoptosis ( J:109623 )

• in cultured neurons, deprivation of neurotrophic factor attenuates apoptosis in homozygous knockout neurons; 6 hours after deprivation, most control neurons are detached from dishes and remaining cells have shrunken while deficient neurons still have neurites and more cells remain attached

• in subventricular zone in wild-type mice after neurotrophic factor deprivation, many TUNEL-positive cells showing nuclear shrinking are detected while there are far fewer (25% vs wild-type) per cerebral hemisphere

increased brain weight ( J:109623 )

• brains from homozygous mice weigh more than wild-type brains

cellular

decreased neuron apoptosis ( J:109623 )

• in cultured neurons, deprivation of neurotrophic factor attenuates apoptosis in homozygous knockout neurons; 6 hours after deprivation, most control neurons are detached from dishes and remaining cells have shrunken while deficient neurons still have neurites and more cells remain attached

• in subventricular zone in wild-type mice after neurotrophic factor deprivation, many TUNEL-positive cells showing nuclear shrinking are detected while there are far fewer (25% vs wild-type) per cerebral hemisphere

Genotype
MGI:3629964

hm2

• Allelic Composition: Ddit3^tm1Aki/Ddit3^tm1Aki
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cellular

decreased apoptosis ( J:76277 )

• treatment with SNAP, a nitric oxide donor, causes ~75% decreased viability in isolated pancreatic islet cells, while there is only a ~5% decrease in Ddit3-deficient neuron viability; higher mitochondrial membrane potential is observed in treated Ddit3-deficient islets than in controls at the same SNAP concentrations