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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lig1tm1Dwm
targeted mutation 1, David W Melton
MGI:2179227
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lig1tm1Dwm/Lig1tm1Dwm involves: 129P2/OlaHsd MGI:3843626
hm2
Lig1tm1Dwm/Lig1tm1Dwm involves: 129P2/OlaHsd * BALB/c MGI:3843612


Genotype
MGI:3843626
hm1
Allelic
Composition
Lig1tm1Dwm/Lig1tm1Dwm
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lig1tm1Dwm mutation (0 available); any Lig1 mutation (48 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
N
• fibroblasts exhibit normal nucleotide excision repair, repair of alkylation damage, sensitivity to 3-aminobenzamide, repair of ionizing radiation damage, and sister chromatid exchange rates
• cultured fibroblasts grow more slowly than wild-type cells
• fibroblasts accumulate replication intermediates unlike in wild-type cells
• fibroblasts exhibit increased chromosomal instability compared to wild-type cells

hematopoietic system
• when fetal liver cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice, fetal liver cells contribute to all lineages but in lower numbers than when wild-type cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice
• when fetal liver cells are used to rescue lethally irradiated mice

homeostasis/metabolism
• fibroblasts accumulate replication intermediates unlike in wild-type cells

immune system
• when fetal liver cells are used to rescue lethally irradiated mice

growth/size/body
• when fetal liver cells are used to rescue lethally irradiated mice




Genotype
MGI:3843612
hm2
Allelic
Composition
Lig1tm1Dwm/Lig1tm1Dwm
Genetic
Background
involves: 129P2/OlaHsd * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lig1tm1Dwm mutation (0 available); any Lig1 mutation (48 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• although mice are present at E15.5, no mice are alive at E16.5

hematopoietic system
• at E15.5, the total number of circulating blood cells is 5% of that in wild-type mice
• the number of primitive multipotent (CFU-A) and erythroid (BFU-E) progenitors in the liver are decreased compared to in wild-type mice
• liver suspensions are unable to rescue lethally irradiated mice
• liver erythropoiesis is disrupted
• however, yolk sac erythropoiesis is normal
• at E15.5
• the number of erythroid (BFU-E) progenitors in the liver are decreased compared to in wild-type mice
• at E15.5, mice lack mature enucleated erythrocytes

embryo

liver/biliary system
• at E15.5, livers lack erythropoietic islands and lack extensive cellularization compared with wild-type livers
• however, hepatocytes and megakaryocytes appear normal
• fewer erythroid hematopoietic cells are found within the liver compared to in wild-type mice
• however, the number of non-erythoid hematopoeitic cells is normal

growth/size/body

integument
• embryos are pale at E11.5





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory