Phenotypes associated with this allele

• Allele Symbol: Rr158^tm1Cgn
• Allele Name: targeted mutation 1, University of Cologne
• Allele ID: MGI:2179291
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rr158^tm1Cgn/Rr158^tm1Cgn	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129S2/SvPas * C57BL/6)	MGI:2179294
cx2	Igh^tm2Cgn/Igh^+ Rr158^tm1Cgn/Rr158^tm1Cgn Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt	involves: 129P2/OlaHsd * C57BL/6	MGI:3687248

Genotype
MGI:2179294

hm1

• Allelic Composition: Rr158^tm1Cgn/Rr158^tm1Cgn
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129S2/SvPas * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell differentiation ( J:12574 , J:64287 )

• light decrease in the number of splenic B cells which express only the lamda chain (expressed in about 5% of wild-type B cells); however spleen size is similar to wild-type (J:12574)

• complete inhibition of immunoglobulin kappa chain V-J rearrangement (J:12574)

• complete inhibition of immunoglobulin kappa chain V-J rearrangement (J:64287)

decreased mature B cell number ( J:64287 )

• 2.3 fold less B cells in the spleen

• B cells expressing kappa chain completely absent in the spleen

• the percentage of newly generated B cell is half that of wild-type

increased mature B cell number ( J:64287 )

• 7 fold increase of lambda-chain expressing B cells in the bone marrow

spleen hypoplasia ( J:64287 )

• 30% fewer nucleated cells found in the spleen

hematopoietic system

abnormal B cell differentiation ( J:12574 , J:64287 )

• light decrease in the number of splenic B cells which express only the lamda chain (expressed in about 5% of wild-type B cells); however spleen size is similar to wild-type (J:12574)

• complete inhibition of immunoglobulin kappa chain V-J rearrangement (J:12574)

• complete inhibition of immunoglobulin kappa chain V-J rearrangement (J:64287)

decreased mature B cell number ( J:64287 )

• 2.3 fold less B cells in the spleen

• B cells expressing kappa chain completely absent in the spleen

• the percentage of newly generated B cell is half that of wild-type

increased mature B cell number ( J:64287 )

• 7 fold increase of lambda-chain expressing B cells in the bone marrow

spleen hypoplasia ( J:64287 )

• 30% fewer nucleated cells found in the spleen

Genotype
MGI:3687248

cx2

• Allelic Composition: Igh^tm2Cgn/Igh⁺; Rr158^tm1Cgn/Rr158^tm1Cgn; Arhgef1^tm1.1Rmt/Arhgef1^tm1.1Rmt
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cellular

abnormal B cell migration ( J:113295 )

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations

• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type

• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness

immune system

abnormal B cell migration ( J:113295 )

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations

• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type

• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness

hematopoietic system

abnormal B cell migration ( J:113295 )

• increase in the proportion of cells that migrate towards high sphingosine 1-phosphate (S1P) concentrations

• reduction in migratory response towards S1P induced by LPS treatment is decreased compared to wild-type

• immunization with a low dose of T cell dependent antigen (NP-CG) fails to suppress responsiveness to S1P unlike in wild-type mice; however, treatment with a high concentration of antigen does reduce S1P responsiveness