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Allele Symbol Allele Name Allele ID |
Onecut1tm1Flem targeted mutation 1, Frederic P Lemaigre MGI:2179340 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• pancreatic buds smaller and less lobulated by E12.5
• dorsal bud more elongated and flattened with about half the normal number of cells at E10.75
• at the same time the ventral pancreas is not visible
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 75% die between day 1 and day 10
• remaining 25% reach adulthood
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• enlarged structures form connecting liver to duodenum rather than individualized hepatic ducts
• duct-like structures formed around E14.5 becoming large cysts by E16.5
• almost completely regressed by age 10 days
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• biliary cysts are seen in the vicinity of the portal vein in embryos
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• gall bladder missing as early as E10.5
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• hepatoblast differentiation is perturbed such that they differentiate into hybrid cells that display characteristics of both hepatocytes and biliary cells
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• biliary cysts are seen in the vicinity of the portal vein in embryos
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• reduced growth rate after birth
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• growth retarded at birth
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• enlarged structures form connecting liver to duodenum rather than individualized hepatic ducts
• duct-like structures formed around E14.5 becoming large cysts by E16.5
• almost completely regressed by age 10 days
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• biliary cysts are seen in the vicinity of the portal vein in embryos
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• gall bladder missing as early as E10.5
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• number of Neurog3 expressing cells is reduced relative to controls at E14.5; pancreas sections almost completely have 4 Neurog3-expressing cells/section compared to 56 cells/section in wild-type
• very few insulin or glucagon-expressing cells are detected
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• Islets do not become organized until 2-3 weeks of age
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• number of glucagon expressing cells is reduced 85% at E12.5 and E15.5
• number of glucagon cell clusters is normal at the same time
• marked reduction in the number of alpha cells at 4 days of age
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• glucagon-expressing cells are almost absent
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• marked reduction in numbers of beta cells at 4 days of age
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• insulin-expressing cells are almost absent
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• marked reduction in the number of delta cells at 4 days of age
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• abnormal architecture, alpha cells scattered rather than forming a peripheral mantle
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• at about E10.5, ventral pancreatic bud is missing and dorsal pancreas is smaller relative to wild-type controls
(J:62595)
• at E13.5, pancreatic buds have not fused, dorsal pancreas is smaller than in controls, and the ventral pancreas is only a minute bud in the duodenal loop
(J:62595)
• endocrine differentiation initiates normally at E9.5 and progresses comparably to wild-type at E10.5, but becomes defective by E12.5
(J:62595)
• at E13.5, dorsal pancreas is smaller than in Onecut1-deficient embryos; no ventral bud is observed, instead consisting of two small cell clusters on the duodenum
(J:121610)
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• endocrine differentiation is more affected than in Onecut1-null embryos; pancreas sections almost completely lack Neurog3-expressing cells (~0.5 cells/section)
• endocrine differentiation initiates normally at E9.5 and progresses comparably to wild-type at E10.5, but becomes defective by E12.5
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• mice show pancreas hypoplasia during development
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• blood glucose low in fasted 4 day old mice
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• at 10 weeks, elevated fasting glycemia in a glucose tolerance test
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• glucagons levels reduced 20% in 4 day old fasted mice
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• no insulin at E15.5
• insulin levels undetectable in 4 day old fasted mice
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• at 10 weeks of age
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• hepatoblast differentiation is perturbed such that they differentiate into hybrid cells that display characteristics of both hepatocytes and biliary cells
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• cord-like organization of the epithelial cells in the parenchyma is severely perturbed in E15.5 embryos with epithelial cells forming clusters surrounded by hematopoietic cells
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• at E13.5, dorsal pancreas is smaller than in Onecut1-deficient embryos; no ventral bud is observed, instead consisting of two small cell clusters on the duodenum
• endocrine differentiation is more affected than in Onecut1-null embryos; pancreas sections almost completely lack Neurog3-expressing cells (~0.5 cells/section)
• insulin- and glucagon-expressing cells are almost absent
• endocrine differentiation initiates normally at E9.5 and progresses comparably to wild-type at E10.5, but becomes defective by E12.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• number of Neurog3 expressing cells is reduced relative to controls, similar to Onecut1-null embryos at E14. (~4 cells/section)
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• pancreas hypoplasia is not more severe than in Onecut1 homozygotes
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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