Phenotypes associated with this allele

• Allele Symbol: Ccr6^tm1(EGFP)Irw
• Allele Name: targeted mutation 1, Ifor R Williams
• Allele ID: MGI:2179543
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccr6^tm1(EGFP)Irw/Ccr6^tm1(EGFP)Irw	B6.129S6-Ccr6^tm1(EGFP)Irw	MGI:3852186
hm2	Ccr6^tm1(EGFP)Irw/Ccr6^tm1(EGFP)Irw	involves: 129S6/SvEvTac * C57BL/6	MGI:3852161

Genotype
MGI:3852186

hm1

• Allelic Composition: Ccr6^tm1(EGFP)Irw/Ccr6^tm1(EGFP)Irw
• Genetic Background: B6.129S6-Ccr6^tm1(EGFP)Irw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased interferon-gamma secretion ( J:132012 )

• splenocytes from mice infected with Y. enterocolitica produce much less IFN-gamma than controls

decreased interleukin-12 secretion ( J:132012 )

• splenocytes from mice infected with Y. enterocolitica produce much less IL-12 than controls

decreased interleukin-4 secretion ( J:132012 )

• splenocytes from mice infected with Y. enterocolitica produce much less IL-4 than controls

decreased tumor necrosis factor secretion ( J:132012 )

• splenocytes from mice infected with Y. enterocolitica produce much less TFN than controls

decreased susceptibility to bacterial infection ( J:132012 )

• mice are resistant to oral Y. enterocolitica infection

• six days after infection wild-type mice appear humpbacked and shaggy with large Peyer's patches and lesions present in the intestines, liver and spleen

• mutant mice do not display these infectious symptoms even with 20-fold higher oral doses

• splenocytes from infected mice make much less IL-4, IL-12, TNF, and IFN-gamma than wild-type controls

• mutant mice do become sick after i.p. infection with Y. enterocolitica

Genotype
MGI:3852161

hm2

• Allelic Composition: Ccr6^tm1(EGFP)Irw/Ccr6^tm1(EGFP)Irw
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

immune system

decreased B cell number ( J:120122 )

• Peyer's patches have fewer domes (mean of 1.5 vs. 3.5 in controls) with less total surface area

abnormal alpha-beta intraepithelial T cell morphology ( J:85086 )

• small intestine alpha-beta intraepithelial lymphocyte (IEL) numbers are increased 2.7 fold

abnormal CD4-positive, alpha-beta intraepithelial T cell morphology ( J:85086 )

• CD4⁺ CD8alpha-alpha IEL numbers are increased 6.3-fold

• CD4⁺ IEL numbers are increased 2.1 fold

abnormal CD8 positive, alpha-beta intraepithelial T cell morphology ( J:85086 )

• CD4^- CD8alpha-alpha IEL numbers are increased 3.2-fold

• CD4⁺ CD8alpha-alpha IEL numbers are increased 6.3-fold

• CD8alphabeta IEL numbers are increased 2-fold

abnormal B cell physiology ( J:120122 )

• bone marrow chimera experiments demonstrate that mutant B cells lack the ability to develop isolated lymphoid follicles from cryptopatches

impaired B cell migration ( J:120122 )

• when B cells are injected into B-cell deficient hosts ( Igh-J^tm1Cgn homozygotes), mutant B cells are 3-fold less efficient in migrating to Peyer's patches and 2-fold less efficient in migration to cryptopatches compared to controls

abnormal T cell physiology ( J:85086 )

• IEL lytic activity is increased

increased T cell proliferation ( J:85086 )

• proliferation rate of alpha-beta IEL is higher by almost 2-fold compared to controls

• alphabeta T cell proliferation was also increased

abnormal gut-associated lymphoid tissue morphology ( J:113363 , J:120122 )

• after S. typhimurium infection of the gut, dendritic cells fail to migrate to the follicular associated epithelium in the small intestine (J:113363)

• there is a near absence of immature and mature isolated lymphoid follicles (ILF) found in the small intestine (J:120122)

• augmenting ILF development by LTbetaR-Ig treatment only modestly increases mature ILF numbers compared to the 8-fold increase seen in controls (J:120122)

abnormal Peyer's patch morphology ( J:120122 )

• Peyer's patches have fewer domes (mean of 1.5 vs. 3.5 in controls) with less total surface area

decreased Peyer's patch number ( J:120122 )

• totally cellularity of the Peyer's Patch is less than half of controls

• less B cells are found in the Peyer's patches of the small intestine

abnormal follicular dendritic cell physiology ( J:113363 )

• after S. typhimurium infection of the gut, dendritic cells fail to migrate to the follicular associated epithelium in the small intestine

hematopoietic system

decreased B cell number ( J:120122 )

• Peyer's patches have fewer domes (mean of 1.5 vs. 3.5 in controls) with less total surface area

abnormal alpha-beta intraepithelial T cell morphology ( J:85086 )

• small intestine alpha-beta intraepithelial lymphocyte (IEL) numbers are increased 2.7 fold

abnormal CD4-positive, alpha-beta intraepithelial T cell morphology ( J:85086 )

• CD4⁺ CD8alpha-alpha IEL numbers are increased 6.3-fold

• CD4⁺ IEL numbers are increased 2.1 fold

abnormal CD8 positive, alpha-beta intraepithelial T cell morphology ( J:85086 )

• CD4^- CD8alpha-alpha IEL numbers are increased 3.2-fold

• CD4⁺ CD8alpha-alpha IEL numbers are increased 6.3-fold

• CD8alphabeta IEL numbers are increased 2-fold

abnormal B cell physiology ( J:120122 )

• bone marrow chimera experiments demonstrate that mutant B cells lack the ability to develop isolated lymphoid follicles from cryptopatches

impaired B cell migration ( J:120122 )

• when B cells are injected into B-cell deficient hosts ( Igh-J^tm1Cgn homozygotes), mutant B cells are 3-fold less efficient in migrating to Peyer's patches and 2-fold less efficient in migration to cryptopatches compared to controls

abnormal T cell physiology ( J:85086 )

• IEL lytic activity is increased

increased T cell proliferation ( J:85086 )

• proliferation rate of alpha-beta IEL is higher by almost 2-fold compared to controls

• alphabeta T cell proliferation was also increased

cellular

impaired B cell migration ( J:120122 )

• when B cells are injected into B-cell deficient hosts ( Igh-J^tm1Cgn homozygotes), mutant B cells are 3-fold less efficient in migrating to Peyer's patches and 2-fold less efficient in migration to cryptopatches compared to controls

increased T cell proliferation ( J:85086 )

• proliferation rate of alpha-beta IEL is higher by almost 2-fold compared to controls

• alphabeta T cell proliferation was also increased