Phenotypes associated with this allele

• Allele Symbol: Ccr6^tm1Lira
• Allele Name: targeted mutation 1, Sergio A Lira
• Allele ID: MGI:2179552
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccr6^tm1Lira/Ccr6^tm1Lira	B6.129-Ccr6^tm1Lira	MGI:4354631
hm2	Ccr6^tm1Lira/Ccr6^tm1Lira	involves: 129	MGI:3852173
hm3	Ccr6^tm1Lira/Ccr6^tm1Lira	involves: 129 * C57BL/6	MGI:4354628

Genotype
MGI:4354631

hm1

• Allelic Composition: Ccr6^tm1Lira/Ccr6^tm1Lira
• Genetic Background: B6.129-Ccr6^tm1Lira

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

impaired eosinophil recruitment ( J:71096 )

• lungs have 10-fold fewer peribronchial eosinophils after challenge with antigen to the airway compared to controls

decreased IgE level ( J:71096 )

• there are 10-fold lower circulating IgE levels in mice that have had antigen challenge to their airways compared to challenged wild-type mice

decreased susceptibility to type I hypersensitivity reaction ( J:71096 )

• mice have reduced airway hyperreactivity after antigen challenge

• airway resistance is 2-3 fold lower at its peak compared to controls

• decreased resistance compared to controls is seen up to 48 hours after antigen challenge

decreased interleukin-5 secretion ( J:71096 )

• antigen challenged lungs secrete 5- to 8- fold less IL-5 compared to controls

hematopoietic system

impaired eosinophil recruitment ( J:71096 )

• lungs have 10-fold fewer peribronchial eosinophils after challenge with antigen to the airway compared to controls

decreased IgE level ( J:71096 )

• there are 10-fold lower circulating IgE levels in mice that have had antigen challenge to their airways compared to challenged wild-type mice

Genotype
MGI:3852173

hm2

• Allelic Composition: Ccr6^tm1Lira/Ccr6^tm1Lira
• Genetic Background: involves: 129

immune system

abnormal T cell physiology ( J:123150 )

• transgenic T cells transferred into mutant mice fail to respond to bacteria

• transgenic T cells respond normally to soluble antigen derived from infecting bacteria

• T cells also respond to heat-killed bacteria

• T cell response to soluble antigen can be restored by transfusion of wild-type CD11b⁺ leukocyte phagocytes

abnormal Peyer's patch T cell area morphology ( J:113363 )

• S. typhimurium-specific T cells fail to expand in the Peyer's patch when transferred into mutant hosts that have been orally infected by bacteria

abnormal follicular dendritic cell antigen presentation ( J:113363 )

• after S. typhimurium infection of the gut, dendritic cells fail to migrate and present antigen to T cells in the follicular associated epithelium in the small intestine

hematopoietic system

abnormal T cell physiology ( J:123150 )

• transgenic T cells transferred into mutant mice fail to respond to bacteria

• transgenic T cells respond normally to soluble antigen derived from infecting bacteria

• T cells also respond to heat-killed bacteria

• T cell response to soluble antigen can be restored by transfusion of wild-type CD11b⁺ leukocyte phagocytes

Genotype
MGI:4354628

hm3

• Allelic Composition: Ccr6^tm1Lira/Ccr6^tm1Lira
• Genetic Background: involves: 129 * C57BL/6

immune system

abnormal myeloid dendritic cell morphology ( J:124358 )

• myeloid dendritic cells are reduced by about half in the peritoneal cavity

• myeloid dendritic cells stay low during an inflammatory response to experimentally induced peritonitis

abnormal lymphocyte morphology ( J:148286 )

increased B-1 B cell number ( J:124358 )

• B1-B cell numbers are increased in the peritoneal cavity

decreased B cell number ( J:148286 )

• numbers of B cells in the brain of unimmunized (i.e. no EAE) mice are about half that of wild-type controls

decreased CD8-positive, alpha-beta T cell number ( J:148286 )

• numbers of CD8 T cells in the brain of unimmunized (i.e. no EAE) mice are reduced compared to wild-type controls

abnormal intraepithelial T cell number ( J:76461 )

• alpha-beta IEL numbers in the small intestine are increased 2-fold

• CD69⁺ IEL are increased 6-fold, CD4⁺ IEL are increased 9 fold and CD4⁺CD8⁺ IEL are increased 10-fold

increased T cell number ( J:76461 )

• in addition to increased IEL numbers, lamina propia T cell numbers are also increased

increased double-positive T cell number ( J:76461 )

• CD4⁺CD8⁺ IEL are increased 10-fold in small intestine

increased activated T cell number ( J:76461 )

• CD69⁺ IEL are increased 6-fold in the small intestine

abnormal CD4-positive, alpha beta T cell morphology ( J:148286 )

• no Th17 T cells are detected in the CNS of mice with EAE while being readily found in wild-type mice with EAE

decreased CD4-positive, alpha-beta T cell number ( J:148286 )

• numbers of CD4 T cells in the brain of unimmunized (i.e. no EAE) mice are about half that of wild-type controls

abnormal peritoneal macrophage morphology ( J:124358 )

• peritoneal macrophage numbers are reduced by about a third

decreased IgA level ( J:76461 )

• mice produce less rotavirus-specific IgA in response to infection compared to controls

decreased IgG3 level ( J:76461 )

• mice produce significantly less total and antigen-specific levels of IgG3 in response to immunization

increased IgM level ( J:76461 )

• antigen-specific IgM levels are higher than controls after immunization

abnormal CD4-positive, alpha-beta T cell physiology ( J:148286 )

• mutant Th17 T cells migrate slower across the blood-brain barrier than their wild-type counterparts in mixed bone marrow chimeras

• 7 days after transfer, 90% of transferred T cells in the brain are wild-type

• 16 days after transfer, proportions of wild-type T cells to knockout T cells are 60% vs 40%

abnormal macrophage physiology ( J:124358 )

• peritoneal macrophage response to LPS is attenuated

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased macrophage cytokine production ( J:124358 )

• CCL2, CCL3, and CXCL10 production by peritoneal macrophages is attenuated in response to LPS

• KC, CCL2, CCL3, CXCL10 production is also reduced in mice undergoing peritonitis with lower levels detected in sera and peritoneal cavities

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased macrophage nitric oxide production ( J:124358 )

• NO production by peritoneal macrophages is attenuated in response to LPS or LPS+ IFN-gamma

abnormal gut-associated lymphoid tissue morphology ( J:76461 )

• in addition to increased IEL numbers, lamina propia T cell numbers are also increased

• after oral immunization, there are fewer antibody producing cells found in the lamina propia compared to controls

abnormal Peyer's patch morphology ( J:76461 )

• Peyer's patch lack the CD11b⁺ CD11c⁺ dendritic cell subset

abnormal Peyer's patch germinal center morphology ( J:76461 )

• after oral immunization, there are fewer antibody producing cells found in Peyer's patches compared to controls

• both IgA and IgG producing cells are affected and similar findings are seen whether KLH- or CT- specific antibody-producing cells are assayed

decreased interleukin-10 secretion ( J:124358 )

• IL-10 production by peritoneal macrophages is attenuated in response to LPS

• IL-10 production is also reduced in mice undergoing peritonitis with lower levels detected in sera and peritoneal cavities

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased interleukin-12 secretion ( J:124358 )

• IL-12p70 production by peritoneal macrophages is attenuated in response to LPS

• IL12-p70 production is also reduced in mice undergoing peritonitis with lower levels detected in sera and peritoneal cavities

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased tumor necrosis factor secretion ( J:124358 )

• TNF production by peritoneal macrophages is attenuated in response to LPS

• TNF production is also reduced in mice undergoing peritonitis with lower levels detected in sera and peritoneal cavities

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:148286 )

• are completely resistant to the development of MOG-induced EAE and do not show leukocyte infiltration in the CNS, whereas a few (8 of 26) have only minimal disease

• EAE can be induced when wild-type T cells are transfused into mutant recipient mice

decreased inflammatory response ( J:124358 )

• mice have increased survival to experimental peritonitis induced by cecal ligation and puncture (CLP)

• 62% of mutant mice are alive six days after CLP while all of control mice are dead by 3 days

• antibiotic treatment reduces lethality for both groups but mutant mice still have a higher survival rate with 88% alive after 6 days compared 25% of wild-type mice

increased susceptibility to Riboviria infection ( J:76461 )

• mice have delayed clearance of rotavirus

• mice have 2-fold higher virus in stools 5 to 6 days after infection compared to controls

hematopoietic system

abnormal myeloid dendritic cell morphology ( J:124358 )

• myeloid dendritic cells are reduced by about half in the peritoneal cavity

• myeloid dendritic cells stay low during an inflammatory response to experimentally induced peritonitis

abnormal lymphocyte morphology ( J:148286 )

increased B-1 B cell number ( J:124358 )

• B1-B cell numbers are increased in the peritoneal cavity

decreased B cell number ( J:148286 )

• numbers of B cells in the brain of unimmunized (i.e. no EAE) mice are about half that of wild-type controls

decreased CD8-positive, alpha-beta T cell number ( J:148286 )

• numbers of CD8 T cells in the brain of unimmunized (i.e. no EAE) mice are reduced compared to wild-type controls

abnormal intraepithelial T cell number ( J:76461 )

• alpha-beta IEL numbers in the small intestine are increased 2-fold

• CD69⁺ IEL are increased 6-fold, CD4⁺ IEL are increased 9 fold and CD4⁺CD8⁺ IEL are increased 10-fold

increased T cell number ( J:76461 )

• in addition to increased IEL numbers, lamina propia T cell numbers are also increased

increased double-positive T cell number ( J:76461 )

• CD4⁺CD8⁺ IEL are increased 10-fold in small intestine

increased activated T cell number ( J:76461 )

• CD69⁺ IEL are increased 6-fold in the small intestine

abnormal CD4-positive, alpha beta T cell morphology ( J:148286 )

• no Th17 T cells are detected in the CNS of mice with EAE while being readily found in wild-type mice with EAE

decreased CD4-positive, alpha-beta T cell number ( J:148286 )

• numbers of CD4 T cells in the brain of unimmunized (i.e. no EAE) mice are about half that of wild-type controls

abnormal peritoneal macrophage morphology ( J:124358 )

• peritoneal macrophage numbers are reduced by about a third

decreased IgA level ( J:76461 )

• mice produce less rotavirus-specific IgA in response to infection compared to controls

decreased IgG3 level ( J:76461 )

• mice produce significantly less total and antigen-specific levels of IgG3 in response to immunization

increased IgM level ( J:76461 )

• antigen-specific IgM levels are higher than controls after immunization

abnormal CD4-positive, alpha-beta T cell physiology ( J:148286 )

• mutant Th17 T cells migrate slower across the blood-brain barrier than their wild-type counterparts in mixed bone marrow chimeras

• 7 days after transfer, 90% of transferred T cells in the brain are wild-type

• 16 days after transfer, proportions of wild-type T cells to knockout T cells are 60% vs 40%

abnormal macrophage physiology ( J:124358 )

• peritoneal macrophage response to LPS is attenuated

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased macrophage cytokine production ( J:124358 )

• CCL2, CCL3, and CXCL10 production by peritoneal macrophages is attenuated in response to LPS

• KC, CCL2, CCL3, CXCL10 production is also reduced in mice undergoing peritonitis with lower levels detected in sera and peritoneal cavities

• peritoneal macrophage response to LPS is enhanced when cells are isolated from mice with peritonitis

decreased macrophage nitric oxide production ( J:124358 )

• NO production by peritoneal macrophages is attenuated in response to LPS or LPS+ IFN-gamma

nervous system

abnormal choroid plexus morphology ( J:148286 )

• CD45⁺ cells accumulate in the choroid plexus parenchyma after MOG immunization

homeostasis/metabolism

decreased macrophage nitric oxide production ( J:124358 )

• NO production by peritoneal macrophages is attenuated in response to LPS or LPS+ IFN-gamma