Phenotypes associated with this allele

• Allele Symbol: Prkcd^tm1Kin
• Allele Name: targeted mutation 1, Kei-ichi Nakayama
• Allele ID: MGI:2179710
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prkcd^tm1Kin/Prkcd^tm1Kin	involves: 129P2/OlaHsd	MGI:3841727
hm2	Prkcd^tm1Kin/Prkcd^tm1Kin	involves: 129P2/OlaHsd * C57BL/6	MGI:3841648
cx3	Blnk^tm1Dkit/Blnk^tm1Dkit Prkcd^tm1Kin/Prkcd^tm1Kin	involves: 129P2/OlaHsd * C57BL/6 * NZB	MGI:3841693

Genotype
MGI:3841727

hm1

• Allelic Composition: Prkcd^tm1Kin/Prkcd^tm1Kin
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal osteoblast differentiation ( J:117333 )

• as determined by marker expression, osteoblast differentiation is impeded compared to in wild-type mice

• at E13.5, cultured limb priomordial cells produce fewer bone nodules and more cartilage nodules than formed by wild-type cells

decreased width of hypertrophic chondrocyte zone ( J:117333 )

abnormal bone ossification ( J:117333 )

• at E15.5, mice exhibit less ossification compared to wild-type mice

• at E15.5, mineralization of the maxilla and mandible is minimal compared to in wild-type mice

• at E14.5 and E15.5, bone collars of ossifying skeletal elements, specifically long bones, are shorter than in wild-type mice

cellular

abnormal osteoblast differentiation ( J:117333 )

• as determined by marker expression, osteoblast differentiation is impeded compared to in wild-type mice

• at E13.5, cultured limb priomordial cells produce fewer bone nodules and more cartilage nodules than formed by wild-type cells

Genotype
MGI:3841648

hm2

• Allelic Composition: Prkcd^tm1Kin/Prkcd^tm1Kin
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

increased B cell proliferation ( J:76135 , J:143854 )

• in response to F(ab)s specific for IgM and antibodies to CD40 or LSP (J:76135)

• when transferred into Rag null mice, more B220+ IgM+ cells compared to when wild-type cells are transplanted (J:76135)

• when stimulated with anti-CD40 antibodies or anti-IgM antibodies (J:143854)

enlarged spleen ( J:76135 )

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

increased B cell number ( J:76135 )

• the number of IgM+ TCR(beta)- B220+ cells are increased in the spleen and lymph nodes compared to in wild-type mice

• however, the numbers of splenic and peritoneal CD5+ B lymphocytes are normal

• when transferred into Rag null mice, more B220+ IgM+ cells compared to when wild-type cells are transplanted

increased transitional stage B cell number ( J:143854 )

• T1 and T2 B cells are increased 2- to 3-fold compared to in wild-type mice

increased follicular B cell number ( J:143854 )

• 2- to 3-fold

increased marginal zone B cell number ( J:143854 )

• 2- to 3-fold

increased spleen germinal center number ( J:76135 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:76135 , J:143854 )

increased IgG1 level ( J:76135 , J:143854 )

increased IgM level ( J:143854 )

abnormal Peyer's patch germinal center morphology ( J:76135 )

• increased compared to in wild-type mice

abnormal lymph node germinal center morphology ( J:76135 )

• increased compared to in wild-type mice

enlarged lymph nodes ( J:76135 )

increased interleukin-6 secretion ( J:76135 )

• from stimulated B cells

increased anti-chromatin antibody level ( J:76135 )

• after 6 months

increased acute inflammation ( J:76135 )

• perivascular infiltration of leukocytes, comprised mostly of B cells, is observed in the kidney, liver, lungs, and salivary glands unlike in wild-type mice

glomerulonephritis ( J:76135 )

• glomeruli are enlarged and stain positive for IgG and C3 in the mesangial region and along capillary walls unlike in wild-type mice

renal/urinary system

glomerulonephritis ( J:76135 )

• glomeruli are enlarged and stain positive for IgG and C3 in the mesangial region and along capillary walls unlike in wild-type mice

renal glomerular immunoglobulin deposits ( J:76135 )

endocrine/exocrine glands

abnormal pancreatic beta cell morphology ( J:120293 )

• the total number of insulin granules is greater than in wild-type beta-cells

• following glucose treatment, the number of granules within 100 nm or 100-200 nm from the plasma membrane is increased compared to in wild-type beta-cells

decreased insulin secretion ( J:120293 )

• mice exhibit reduced glucose-stimulated insulin secretion compared to in wild-type mice

• islet cells stimulated with glucose-, KCl-, or phorbol 12-myristate 13-acetate with or without forskolin exhibit decreased insulin secretion compared to in wild-type cells

growth/size/body

decreased body weight ( J:120293 )

• modestly

enlarged spleen ( J:76135 )

hematopoietic system

increased B cell proliferation ( J:76135 , J:143854 )

• in response to F(ab)s specific for IgM and antibodies to CD40 or LSP (J:76135)

• when transferred into Rag null mice, more B220+ IgM+ cells compared to when wild-type cells are transplanted (J:76135)

• when stimulated with anti-CD40 antibodies or anti-IgM antibodies (J:143854)

enlarged spleen ( J:76135 )

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

increased B cell number ( J:76135 )

• the number of IgM+ TCR(beta)- B220+ cells are increased in the spleen and lymph nodes compared to in wild-type mice

• however, the numbers of splenic and peritoneal CD5+ B lymphocytes are normal

• when transferred into Rag null mice, more B220+ IgM+ cells compared to when wild-type cells are transplanted

increased transitional stage B cell number ( J:143854 )

• T1 and T2 B cells are increased 2- to 3-fold compared to in wild-type mice

increased follicular B cell number ( J:143854 )

• 2- to 3-fold

increased marginal zone B cell number ( J:143854 )

• 2- to 3-fold

increased spleen germinal center number ( J:76135 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:76135 , J:143854 )

increased IgG1 level ( J:76135 , J:143854 )

increased IgM level ( J:143854 )

homeostasis/metabolism

decreased insulin secretion ( J:120293 )

• mice exhibit reduced glucose-stimulated insulin secretion compared to in wild-type mice

• islet cells stimulated with glucose-, KCl-, or phorbol 12-myristate 13-acetate with or without forskolin exhibit decreased insulin secretion compared to in wild-type cells

impaired glucose tolerance ( J:120293 )

• from 8 to 20 weeks with no decrease in insulin sensitivity

cellular

increased B cell proliferation ( J:76135 , J:143854 )

• in response to F(ab)s specific for IgM and antibodies to CD40 or LSP (J:76135)

• when transferred into Rag null mice, more B220+ IgM+ cells compared to when wild-type cells are transplanted (J:76135)

• when stimulated with anti-CD40 antibodies or anti-IgM antibodies (J:143854)

Genotype
MGI:3841693

cx3

• Allelic Composition: Blnk^tm1Dkit/Blnk^tm1Dkit; Prkcd^tm1Kin/Prkcd^tm1Kin
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * NZB

immune system

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit moderately enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• as in Blnk^tm1Dkit homozygotes

decreased B-1 B cell number ( J:143854 )

• in the peritoneal cavity

decreased B-2 B cell number ( J:143854 )

• in the peritoneal cavity

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG1 level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG2b level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG3 level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

increased IgM level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

hematopoietic system

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies

abnormal plasma cell differentiation ( J:143854 )

• anti-CD40- and IL4-stimulated splenic B cells exhibit moderately enhanced differentiation into IgG producing plasma cells compared to in wild-type mice

arrested B cell differentiation ( J:143854 )

• as in Blnk^tm1Dkit homozygotes

decreased B-1 B cell number ( J:143854 )

• in the peritoneal cavity

decreased B-2 B cell number ( J:143854 )

• in the peritoneal cavity

decreased follicular B cell number ( J:143854 )

decreased marginal zone B cell number ( J:143854 )

abnormal immunoglobulin level ( J:143854 )

increased IgA level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG1 level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG2b level ( J:143854 )

• higher than in Blnk^tm1Dkit homozygotes

increased IgG3 level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

increased IgM level ( J:143854 )

• 10-fold higher than in Blnk^tm1Dkit homozygotes

cellular

increased B cell apoptosis ( J:143854 )

abnormal B cell proliferation ( J:143854 )

• mice exhibit poor response to anti-IgM antibodies or LPS but a stronger than normal response to anti-CD40 antibodies