Analysis Tools|
Allele Symbol Allele Name Allele ID |
Vamp2tm1Sud targeted mutation 1, Thomas C Sudhof MGI:2179745 |
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| Summary |
8 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• total number of chromaffin vesicles is increased compared to Stx1a mutants
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• total number of chromaffin vesicles is increased compared to Stx1a mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• animals die immediately after birth
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• rounded, curled appearance at birth
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• reduced frequency (about 15% of controls) of spontaneous miniature excitatory currents in hippocampal neurons
• single neurons stimulated at 1 Hz exhibit only rare synaptic events with small amplitudes corresponding to minis; even at high frequencies, only erratic events occur that are not completely synchronous with the stimulation
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• spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose is decreased about 10-fold and fast calcium-triggered fusion is decreased more than 100-fold, indicating that neurotransmitter release is operational but cannot be efficiently stimulated by calcium
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| N |
• homozygotes exhibit normal degranulation of mast cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 2 months of age, mice show a significant reduction in body weight relative to wild-type controls
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• mice lag behind in postnatal growth relative to wild-type controls
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• mice show a significant increase in the number of open arm visits and % of time spent on the open arms in the elevated plus-maze, indicating decreased anxiety-related behavior
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• input-output relationship of fEPSPs of hippocampal CA3-CA1 synapses versus fiber volley amplitudes over a range of stimulation intensities indicates that basal excitatory neurotransmission is reduced relative to wild-type controls
• the ratios of maximal fEPSP amplitudes over the amplitudes of the pre-spike are significantly decreased relative to wild-type controls
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• similar stimulation intensities evoke comparable presynaptic volleys but reduced fEPSPs relative to wild-type controls
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• acute hippocampal slices show increased paired-pulse facilitation (PPF) of fEPSPs relative to wild-type controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• extent and time course of thrombin-induced secretion is comparable to wild-type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in hippocampal neurons from double knockout mice, frequency of miniEPSCs is ~8-fold lower than wild-type, but not different from Vamp2-single knockouts
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• double knockouts do not show a decrease in neurotransmitter release beyond that seen in Vamp2 single knockouts
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• thrombin response curves and kinetics of release in platelets are not different from wild-type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• remaining mice die shortly after P1, with an average death date of 2.4 +/- 0.6 days
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• >50% of mice born die within the first day of birth
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• mice are born well below Mendelian ratios: only 4.6% versus expected 12.5% newborns are observed
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 2 months of age, mice show a similar reduction in body weight as single Vamp2tm1Sud heterozygotes
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• mice lag behind in postnatal growth relative to wild-type controls
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• in the elevated plus-maze test, mice show a higher increase in the number of open arm visits and % of time spent on open arms than single Vamp2tm1Sud heterozygotes, indicating a greater reduction in anxiety-related behavior
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• mice display a stronger reduction in basal synaptic transmission than single Vamp2tm1Sud heterozygotes
• the ratios of maximal fEPSP amplitudes over the amplitudes of the pre-spike are significantly lower than those observed in single Vamp2tm1Sud heterozygotes
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• mice show a stronger facilitation of PPF than single Vamp2tm1Sud heterozygotes
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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